Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats
Renal ischemia-reperfusion (I/R) injury is a common but severe scientific problem. Luteolin has great anti-inflammatory and antioxidant effects. In this study, we studied the effect of luteolin on renal I/R injury in rats. Intragastric administration of luteolin or saline was performed in Sprague-Da...
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/2017/9783893 |
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doaj-d1977e90b5524afa9c53cfd517469c202020-11-25T01:08:16ZengHindawi LimitedMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/97838939783893Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in RatsXin Hong0Xiaojing Zhao1Gang Wang2Zhengliang Zhang3Honghong Pei4Zhong Liu5Department of Emergency Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Emergency Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Emergency Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Emergency Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Emergency Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Emergency Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaRenal ischemia-reperfusion (I/R) injury is a common but severe scientific problem. Luteolin has great anti-inflammatory and antioxidant effects. In this study, we studied the effect of luteolin on renal I/R injury in rats. Intragastric administration of luteolin or saline was performed in Sprague-Dawley rats before (40 mg/kg for three days) and after (one day) renal I/R modeling. Kidney and blood samples were harvested to detect the severity of renal injury 24 hours after operation. The results showed that luteolin-treated rats exhibited milder histomorphological changes with lower scores of renal histological lesions; lower blood urea nitrogen and creatinine levels; lower renal malondialdehyde (MDA), 8-oxo-deoxyguanosine (8-OHdG), and myeloperoxidase (MPO) levels; and higher superoxide dismutase (SOD) and catalase (CAT) activities in the kidney. Luteolin attenuated the increased levels of serum and renal tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, renal high mobility group box-1 (HMGB1), and nuclear factor kappa β (NF-κB) expression levels in I/R rats. Furthermore, luteolin treatment significantly reduced renal cell apoptosis and endoplasmic reticulum (ER) stress caused by renal I/R injury. In conclusion, luteolin improved renal function in I/R rats by reducing oxidative stress, neutrophil infiltration, inflammation, renal cell apoptosis, and expression of HMGB1 and NF-κB, and ER stress.http://dx.doi.org/10.1155/2017/9783893 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin Hong Xiaojing Zhao Gang Wang Zhengliang Zhang Honghong Pei Zhong Liu |
spellingShingle |
Xin Hong Xiaojing Zhao Gang Wang Zhengliang Zhang Honghong Pei Zhong Liu Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats Mediators of Inflammation |
author_facet |
Xin Hong Xiaojing Zhao Gang Wang Zhengliang Zhang Honghong Pei Zhong Liu |
author_sort |
Xin Hong |
title |
Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats |
title_short |
Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats |
title_full |
Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats |
title_fullStr |
Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats |
title_full_unstemmed |
Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats |
title_sort |
luteolin treatment protects against renal ischemia-reperfusion injury in rats |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2017-01-01 |
description |
Renal ischemia-reperfusion (I/R) injury is a common but severe scientific problem. Luteolin has great anti-inflammatory and antioxidant effects. In this study, we studied the effect of luteolin on renal I/R injury in rats. Intragastric administration of luteolin or saline was performed in Sprague-Dawley rats before (40 mg/kg for three days) and after (one day) renal I/R modeling. Kidney and blood samples were harvested to detect the severity of renal injury 24 hours after operation. The results showed that luteolin-treated rats exhibited milder histomorphological changes with lower scores of renal histological lesions; lower blood urea nitrogen and creatinine levels; lower renal malondialdehyde (MDA), 8-oxo-deoxyguanosine (8-OHdG), and myeloperoxidase (MPO) levels; and higher superoxide dismutase (SOD) and catalase (CAT) activities in the kidney. Luteolin attenuated the increased levels of serum and renal tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, renal high mobility group box-1 (HMGB1), and nuclear factor kappa β (NF-κB) expression levels in I/R rats. Furthermore, luteolin treatment significantly reduced renal cell apoptosis and endoplasmic reticulum (ER) stress caused by renal I/R injury. In conclusion, luteolin improved renal function in I/R rats by reducing oxidative stress, neutrophil infiltration, inflammation, renal cell apoptosis, and expression of HMGB1 and NF-κB, and ER stress. |
url |
http://dx.doi.org/10.1155/2017/9783893 |
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