Evaluation of gelling agents on anther culture: response of two soybean cultivars
Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation w...
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Instituto de Tecnologia do Paraná (Tecpar)
2007-11-01
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doaj-d1baefa45acd494c85e2a3b18c7009582020-11-24T22:39:01ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242007-11-0150693393910.1590/S1516-89132007000700004Evaluation of gelling agents on anther culture: response of two soybean cultivarsMilena Barcelos CardosoMaria Helena Bodanese-ZanettiniElsa Cristina de MundstockEliane Kaltchuk-SantosAnthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling agents on the frequency of binucleate symmetrical pollens grains and multinucleate/multicellular structures was not significant. About the frequencies of calli and embryogenic calli formed, a significant difference was detected between the cultivars (IAS5= 14.8% and BRS 133=6.6%). Gelling agents showed no effect over these frequencies.<br>Anteras de duas cultivares de soja foram cultivadas em meio de cultura basal B5 longo gelificado com agarose ou Phytagel®. Análises citológicas das anteras foram conduzidas durante os primeiros 45 dias de cultura para avaliar a viabilidade e o estágio de desenvolvimento dos micrósporos. A freqüência de formação de calos foi analisada após 45 dias do início da cultura. A análise da variância da viabilidade do micrósporo mostrou interações significativas de Cultivar X Agente Gelificante X Dias de Cultura. As freqüências de grãos de pólen viáveis diminuíram significativamente com o tempo de cultura, dentro de cada cultivar e agente gelificante testado. A interação Dia X Cultivar foi significante para as freqüências de grãos de pólen binucleados simétricos e estruturas multinucleados/multicelulares. O efeito do agente gelificante na freqüência de grãos de pólen binucleados simétricos e estruturas multinucleados/multicelulares não foi significante. Com relação às freqüências de calos e estruturas embriogênicas formadas, houve diferença significativa entre cultivares (IAS5= 14.8% e BRS 133=6.6%). O agente gelificante não mostrou efeito em tais freqüências.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004Anther cultureGlycine maxculture mediabinucleate pollen grainsmultinucleatecallus formation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Milena Barcelos Cardoso Maria Helena Bodanese-Zanettini Elsa Cristina de Mundstock Eliane Kaltchuk-Santos |
spellingShingle |
Milena Barcelos Cardoso Maria Helena Bodanese-Zanettini Elsa Cristina de Mundstock Eliane Kaltchuk-Santos Evaluation of gelling agents on anther culture: response of two soybean cultivars Brazilian Archives of Biology and Technology Anther culture Glycine max culture media binucleate pollen grains multinucleate callus formation |
author_facet |
Milena Barcelos Cardoso Maria Helena Bodanese-Zanettini Elsa Cristina de Mundstock Eliane Kaltchuk-Santos |
author_sort |
Milena Barcelos Cardoso |
title |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_short |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_full |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_fullStr |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_full_unstemmed |
Evaluation of gelling agents on anther culture: response of two soybean cultivars |
title_sort |
evaluation of gelling agents on anther culture: response of two soybean cultivars |
publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
series |
Brazilian Archives of Biology and Technology |
issn |
1516-8913 1678-4324 |
publishDate |
2007-11-01 |
description |
Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling agents on the frequency of binucleate symmetrical pollens grains and multinucleate/multicellular structures was not significant. About the frequencies of calli and embryogenic calli formed, a significant difference was detected between the cultivars (IAS5= 14.8% and BRS 133=6.6%). Gelling agents showed no effect over these frequencies.<br>Anteras de duas cultivares de soja foram cultivadas em meio de cultura basal B5 longo gelificado com agarose ou Phytagel®. Análises citológicas das anteras foram conduzidas durante os primeiros 45 dias de cultura para avaliar a viabilidade e o estágio de desenvolvimento dos micrósporos. A freqüência de formação de calos foi analisada após 45 dias do início da cultura. A análise da variância da viabilidade do micrósporo mostrou interações significativas de Cultivar X Agente Gelificante X Dias de Cultura. As freqüências de grãos de pólen viáveis diminuíram significativamente com o tempo de cultura, dentro de cada cultivar e agente gelificante testado. A interação Dia X Cultivar foi significante para as freqüências de grãos de pólen binucleados simétricos e estruturas multinucleados/multicelulares. O efeito do agente gelificante na freqüência de grãos de pólen binucleados simétricos e estruturas multinucleados/multicelulares não foi significante. Com relação às freqüências de calos e estruturas embriogênicas formadas, houve diferença significativa entre cultivares (IAS5= 14.8% e BRS 133=6.6%). O agente gelificante não mostrou efeito em tais freqüências. |
topic |
Anther culture Glycine max culture media binucleate pollen grains multinucleate callus formation |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004 |
work_keys_str_mv |
AT milenabarceloscardoso evaluationofgellingagentsonanthercultureresponseoftwosoybeancultivars AT mariahelenabodanesezanettini evaluationofgellingagentsonanthercultureresponseoftwosoybeancultivars AT elsacristinademundstock evaluationofgellingagentsonanthercultureresponseoftwosoybeancultivars AT elianekaltchuksantos evaluationofgellingagentsonanthercultureresponseoftwosoybeancultivars |
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