Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes
The mushroom Pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. In nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). The degrada...
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Sociedade Brasileira de Microbiologia
2012-12-01
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doaj-d1cb61a825294952ac0a5dc783799b8b2020-11-24T23:35:32ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052012-12-0143415081515Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastesJosé Maria Rodrigues da LuzMateus Dias NunesSirlaine Albino PaesDenise Pereira TorresMarliane de Cássia Soares da SilvaMaria Catarina Megumi KasuyaThe mushroom Pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. In nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). The degradation of agricultural wastes involves some enzyme complexes made up of oxidative (laccase, manganese peroxidase and lignin peroxidase) and hydrolytic enzymes (cellulases, xylanases and tanases). Understanding how these enzymes work will help to improve the productivity of mushroom cultures and decrease the potential pollution that can be caused by inadequate discharge of the agroindustrial residues. The objective of this work was to assess the activity of the lignocellulolytic enzymes produced by two P. ostreatus strains (PLO 2 and PLO 6). These strains were used to inoculate samples of coffee husks, eucalyptus sawdust or eucalyptus bark add with or without 20 % rice bran. Every five days after substrate inoculation, the enzyme activity and soluble protein concentration were evaluated. The maximum activity of oxidative enzymes was observed at day 10 after inoculation, and the activity of the hydrolytic enzymes increased during the entire period of the experiment. The results show that substrate composition and colonization time influenced the activity of the lignocellulolytic enzymes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400035laccasecellulasemushroomslignocellulolytic residues |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
José Maria Rodrigues da Luz Mateus Dias Nunes Sirlaine Albino Paes Denise Pereira Torres Marliane de Cássia Soares da Silva Maria Catarina Megumi Kasuya |
spellingShingle |
José Maria Rodrigues da Luz Mateus Dias Nunes Sirlaine Albino Paes Denise Pereira Torres Marliane de Cássia Soares da Silva Maria Catarina Megumi Kasuya Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes Brazilian Journal of Microbiology laccase cellulase mushrooms lignocellulolytic residues |
author_facet |
José Maria Rodrigues da Luz Mateus Dias Nunes Sirlaine Albino Paes Denise Pereira Torres Marliane de Cássia Soares da Silva Maria Catarina Megumi Kasuya |
author_sort |
José Maria Rodrigues da Luz |
title |
Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes |
title_short |
Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes |
title_full |
Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes |
title_fullStr |
Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes |
title_full_unstemmed |
Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes |
title_sort |
lignocellulolytic enzyme production of pleurotus ostreatus growth in agroindustrial wastes |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1517-8382 1678-4405 |
publishDate |
2012-12-01 |
description |
The mushroom Pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. In nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). The degradation of agricultural wastes involves some enzyme complexes made up of oxidative (laccase, manganese peroxidase and lignin peroxidase) and hydrolytic enzymes (cellulases, xylanases and tanases). Understanding how these enzymes work will help to improve the productivity of mushroom cultures and decrease the potential pollution that can be caused by inadequate discharge of the agroindustrial residues. The objective of this work was to assess the activity of the lignocellulolytic enzymes produced by two P. ostreatus strains (PLO 2 and PLO 6). These strains were used to inoculate samples of coffee husks, eucalyptus sawdust or eucalyptus bark add with or without 20 % rice bran. Every five days after substrate inoculation, the enzyme activity and soluble protein concentration were evaluated. The maximum activity of oxidative enzymes was observed at day 10 after inoculation, and the activity of the hydrolytic enzymes increased during the entire period of the experiment. The results show that substrate composition and colonization time influenced the activity of the lignocellulolytic enzymes. |
topic |
laccase cellulase mushrooms lignocellulolytic residues |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400035 |
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