Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes
Objective The study investigated the biological functions and mechanisms for controlling cashmere growth of Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) and decorin (DCN) genes. Methods cDNA library of Liaoning cashmere goat was constructed in early stag...
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doaj-d1cd69a2d6d8423c992458ca4406aceb2020-11-24T20:52:55ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172018-05-0131565065710.5713/ajas.17.051723938Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genesMei Jin0Jun-yan Zhang1Ming-xing Chu2Jun Piao3Jing-ai Piao4Feng-qin Zhao5 Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Department of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, China Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Department of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, China Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 10000, China Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Department of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, China Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Department of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, China Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Department of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, ChinaObjective The study investigated the biological functions and mechanisms for controlling cashmere growth of Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) and decorin (DCN) genes. Methods cDNA library of Liaoning cashmere goat was constructed in early stages. OCIAD2 and DCN genes related to cashmere growth were identified by homology analysis comparison. The expression location of OCIAD2 and DCN genes in primary and secondary hair follicles (SF) was performed using in situ hybridization. The expression of OCIAD2 and DCN genes in primary and SF was performed using real-time polymerase chain reaction (PCR). Results In situ hybridization revealed that OCIAD2 and DCN were expressed in the inner root sheath of Liaoning cashmere goat hair follicles. Real-time quantitative PCR showed that these genes were highly expressed in SF during anagen, while these genes were highly expressed in primary hair follicle in catagen phase. Melatonin (MT) inhibited the expression of OCIAD2 and promoted the expression of DCN. Insulin-like growth factors-1 (IGF-1) inhibited the expression of OCIAD2 and DCN, while fibroblast growth factors 5 (FGF5) promoted the expression of these genes. MT and IGF-1 promoted OCIAD2 synergistically, while MT and FGF5 inhibited the genes simultaneously. MT+IGF-1/MT+FGF5 inhibited DCN gene. RNAi technology showed that OCIAD2 expression was promoted, while that of DCN was inhibited. Conclusion Activation of bone morphogenetic protein (BMP) signaling pathway up-regulated OCIAD2 expression and stimulated SF to control cell proliferation. DCN gene affected hair follicle morphogenesis and periodic changes by promoting transforming growth factor-β (TGF-β) and BMP signaling pathways. OCIAD2 and DCN genes have opposite effects on TGF-β signaling pathway and inhibit each other to affect the hair growth.http://www.ajas.info/upload/pdf/ajas-31-5-650.pdfLiaoning New-breeding Cashmere GoatOCIAD2DCNCashmere Growth Mechanism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mei Jin Jun-yan Zhang Ming-xing Chu Jun Piao Jing-ai Piao Feng-qin Zhao |
spellingShingle |
Mei Jin Jun-yan Zhang Ming-xing Chu Jun Piao Jing-ai Piao Feng-qin Zhao Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes Asian-Australasian Journal of Animal Sciences Liaoning New-breeding Cashmere Goat OCIAD2 DCN Cashmere Growth Mechanism |
author_facet |
Mei Jin Jun-yan Zhang Ming-xing Chu Jun Piao Jing-ai Piao Feng-qin Zhao |
author_sort |
Mei Jin |
title |
Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes |
title_short |
Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes |
title_full |
Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes |
title_fullStr |
Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes |
title_full_unstemmed |
Cashmere growth control in Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes |
title_sort |
cashmere growth control in liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 and decorin genes |
publisher |
Asian-Australasian Association of Animal Production Societies |
series |
Asian-Australasian Journal of Animal Sciences |
issn |
1011-2367 1976-5517 |
publishDate |
2018-05-01 |
description |
Objective The study investigated the biological functions and mechanisms for controlling cashmere growth of Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) and decorin (DCN) genes. Methods cDNA library of Liaoning cashmere goat was constructed in early stages. OCIAD2 and DCN genes related to cashmere growth were identified by homology analysis comparison. The expression location of OCIAD2 and DCN genes in primary and secondary hair follicles (SF) was performed using in situ hybridization. The expression of OCIAD2 and DCN genes in primary and SF was performed using real-time polymerase chain reaction (PCR). Results In situ hybridization revealed that OCIAD2 and DCN were expressed in the inner root sheath of Liaoning cashmere goat hair follicles. Real-time quantitative PCR showed that these genes were highly expressed in SF during anagen, while these genes were highly expressed in primary hair follicle in catagen phase. Melatonin (MT) inhibited the expression of OCIAD2 and promoted the expression of DCN. Insulin-like growth factors-1 (IGF-1) inhibited the expression of OCIAD2 and DCN, while fibroblast growth factors 5 (FGF5) promoted the expression of these genes. MT and IGF-1 promoted OCIAD2 synergistically, while MT and FGF5 inhibited the genes simultaneously. MT+IGF-1/MT+FGF5 inhibited DCN gene. RNAi technology showed that OCIAD2 expression was promoted, while that of DCN was inhibited. Conclusion Activation of bone morphogenetic protein (BMP) signaling pathway up-regulated OCIAD2 expression and stimulated SF to control cell proliferation. DCN gene affected hair follicle morphogenesis and periodic changes by promoting transforming growth factor-β (TGF-β) and BMP signaling pathways. OCIAD2 and DCN genes have opposite effects on TGF-β signaling pathway and inhibit each other to affect the hair growth. |
topic |
Liaoning New-breeding Cashmere Goat OCIAD2 DCN Cashmere Growth Mechanism |
url |
http://www.ajas.info/upload/pdf/ajas-31-5-650.pdf |
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