Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease
Aims: To assess the impact of autologous serum eye drops on the level of ocular surface apoptosis in patients with bilateral severe dry eye disease. Methods: This prospective study was conducted on 10 patients with severe dry eye due to graft versus host disease (group 1) and 6 patients with severe...
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Palacký University Olomouc, Faculty of Medicine and Dentistry
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doaj-d200567a8b754fa28e1cfb8e5ff4f92b2020-11-25T02:55:52ZengPalacký University Olomouc, Faculty of Medicine and DentistryBiomedical Papers1213-81181804-75212016-06-01160227127510.5507/bp.2016.001bio-201602-0014Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye diseaseIvana Rybickova0Viera Vesela1Ivan Fales2Pavlina Skalicka3Katerina Jirsova4Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General University Hospital and First Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicLaboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General University Hospital and First Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicDepartment of Cell Therapy and Cord Blood Bank Czech Republic, Institute of Haematology and Blood Transfusion, Prague, Czech RepublicDepartment of Ophthalmology, General University Hospital and First Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicLaboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General University Hospital and First Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicAims: To assess the impact of autologous serum eye drops on the level of ocular surface apoptosis in patients with bilateral severe dry eye disease. Methods: This prospective study was conducted on 10 patients with severe dry eye due to graft versus host disease (group 1) and 6 patients with severe dry eye due to primary Sjögren's syndrome (group 2). Impression cytology specimens from the bulbar conjunctiva were obtained before and after a three-month treatment with 20% autologous serum eye drops applied a maximum of 12 times a day together with regular therapy with artificial tears. The percentage of apoptotic epithelial cells was evaluated immunochemically using anti-active caspase 3 antibody. Results: In group 1, the mean percentage of apoptotic cells was 3.6% before the treatment. The three-month treatment led to a significant decrease to a mean percentage of 1.8% (P = 0.028). The mean percentage of apoptotic conjunctival cells decreased from 5.4% before the treatment to 3.8% in group 2; however, these results did not reach the level of significance. Conclusion: Three-month autologous serum treatment led to the improvement of ocular surface apoptosis, especially in the group of patients with severe dry eye due to graft versus host disease. This result supports the very positive effect of autologous serum on the ocular surface in patients suffering from severe dry eye.https://biomed.papers.upol.cz/artkey/bio-201602-0014_Apoptosis_of_conjunctival_epithelial_cells_before_and_after_the_application_of_autologous_serum_eye_drops_in_se.phpapoptosisautologous serumdry eye diseasecaspase 3conjunctival epithelium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ivana Rybickova Viera Vesela Ivan Fales Pavlina Skalicka Katerina Jirsova |
spellingShingle |
Ivana Rybickova Viera Vesela Ivan Fales Pavlina Skalicka Katerina Jirsova Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease Biomedical Papers apoptosis autologous serum dry eye disease caspase 3 conjunctival epithelium |
author_facet |
Ivana Rybickova Viera Vesela Ivan Fales Pavlina Skalicka Katerina Jirsova |
author_sort |
Ivana Rybickova |
title |
Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease |
title_short |
Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease |
title_full |
Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease |
title_fullStr |
Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease |
title_full_unstemmed |
Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease |
title_sort |
apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease |
publisher |
Palacký University Olomouc, Faculty of Medicine and Dentistry |
series |
Biomedical Papers |
issn |
1213-8118 1804-7521 |
publishDate |
2016-06-01 |
description |
Aims: To assess the impact of autologous serum eye drops on the level of ocular surface apoptosis in patients with bilateral severe dry eye disease.
Methods: This prospective study was conducted on 10 patients with severe dry eye due to graft versus host disease (group 1) and 6 patients with severe dry eye due to primary Sjögren's syndrome (group 2). Impression cytology specimens from the bulbar conjunctiva were obtained before and after a three-month treatment with 20% autologous serum eye drops applied a maximum of 12 times a day together with regular therapy with artificial tears. The percentage of apoptotic epithelial cells was evaluated immunochemically using anti-active caspase 3 antibody.
Results: In group 1, the mean percentage of apoptotic cells was 3.6% before the treatment. The three-month treatment led to a significant decrease to a mean percentage of 1.8% (P = 0.028). The mean percentage of apoptotic conjunctival cells decreased from 5.4% before the treatment to 3.8% in group 2; however, these results did not reach the level of significance.
Conclusion: Three-month autologous serum treatment led to the improvement of ocular surface apoptosis, especially in the group of patients with severe dry eye due to graft versus host disease. This result supports the very positive effect of autologous serum on the ocular surface in patients suffering from severe dry eye. |
topic |
apoptosis autologous serum dry eye disease caspase 3 conjunctival epithelium |
url |
https://biomed.papers.upol.cz/artkey/bio-201602-0014_Apoptosis_of_conjunctival_epithelial_cells_before_and_after_the_application_of_autologous_serum_eye_drops_in_se.php |
work_keys_str_mv |
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