Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, mo...

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Bibliographic Details
Main Authors: Lilian Giotto Zaros, Patrízia Ana Bricarello, Alessandro Francisco Talamini Amarante, Luiz Lehmann Coutinho
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2007-01-01
Series:Genetics and Molecular Biology
Subjects:
cytokine gene expression
real-time RT-PCR
cattle
gastrointestinal nematodes
bovine cytokines
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012
Description
Summary:T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.
ISSN:1415-4757
1678-4685

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