Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, mo...

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Main Authors:	Lilian Giotto Zaros, Patrízia Ana Bricarello, Alessandro Francisco Talamini Amarante, Luiz Lehmann Coutinho
Format:	Article
Language:	English
Published:	Sociedade Brasileira de Genética 2007-01-01
Series:	Genetics and Molecular Biology
Subjects:	cytokine gene expression real-time RT-PCR cattle gastrointestinal nematodes bovine cytokines
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012

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spelling	doaj-d2036b37366343d19601df83e1e854f42020-11-25T00:43:35ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852007-01-0130357557910.1590/S1415-47572007000400012Quantification of bovine cytokine gene expression using real-time RT-PCR methodologyLilian Giotto ZarosPatrízia Ana BricarelloAlessandro Francisco Talamini AmaranteLuiz Lehmann CoutinhoT cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012cytokine gene expressionreal-time RT-PCRcattlegastrointestinal nematodesbovine cytokines
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Lilian Giotto Zaros Patrízia Ana Bricarello Alessandro Francisco Talamini Amarante Luiz Lehmann Coutinho
spellingShingle	Lilian Giotto Zaros Patrízia Ana Bricarello Alessandro Francisco Talamini Amarante Luiz Lehmann Coutinho Quantification of bovine cytokine gene expression using real-time RT-PCR methodology Genetics and Molecular Biology cytokine gene expression real-time RT-PCR cattle gastrointestinal nematodes bovine cytokines
author_facet	Lilian Giotto Zaros Patrízia Ana Bricarello Alessandro Francisco Talamini Amarante Luiz Lehmann Coutinho
author_sort	Lilian Giotto Zaros
title	Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_short	Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_full	Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_fullStr	Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_full_unstemmed	Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_sort	quantification of bovine cytokine gene expression using real-time rt-pcr methodology
publisher	Sociedade Brasileira de Genética
series	Genetics and Molecular Biology
issn	1415-4757 1678-4685
publishDate	2007-01-01
description	T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.
topic	cytokine gene expression real-time RT-PCR cattle gastrointestinal nematodes bovine cytokines
url	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012
work_keys_str_mv	AT liliangiottozaros quantificationofbovinecytokinegeneexpressionusingrealtimertpcrmethodology AT patriziaanabricarello quantificationofbovinecytokinegeneexpressionusingrealtimertpcrmethodology AT alessandrofranciscotalaminiamarante quantificationofbovinecytokinegeneexpressionusingrealtimertpcrmethodology AT luizlehmanncoutinho quantificationofbovinecytokinegeneexpressionusingrealtimertpcrmethodology
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Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

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