Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, mo...

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Bibliographic Details
Main Authors: Lilian Giotto Zaros, Patrízia Ana Bricarello, Alessandro Francisco Talamini Amarante, Luiz Lehmann Coutinho
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2007-01-01
Series:Genetics and Molecular Biology
Subjects:
cytokine gene expression
real-time RT-PCR
cattle
gastrointestinal nematodes
bovine cytokines
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012

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