Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods
Hydatid cyst, the larval stage of cestodes Echinococcus spp., is recognized as a zoonotic infection in the world. The World Health Organization (WHO) has recently classified echinococcosis in a group of neglected tropical diseases. The prevalence of Echinococcus granulosus infection is high in Iran...
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doaj-d211ccc7e63b41a4a30545cf0a2e5ab82020-11-25T01:14:49Zeng Razi Vaccine and Serum Research InstituteArchives of Razi Institute 0365-34392008-98722016-07-01712103108Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methodsShirazi, S.0Madani, R.1Hoghooghi Rad, N.2Ranjbar Bahadori, S.3Department of Pathobiology, Faculty of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Proteomics, Biochemistry and Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Pathobiology, Faculty of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Pathobiology, Faculty of Veterinary, Islamic Azad University, Garmsar, IranHydatid cyst, the larval stage of cestodes Echinococcus spp., is recognized as a zoonotic infection in the world. The World Health Organization (WHO) has recently classified echinococcosis in a group of neglected tropical diseases. The prevalence of Echinococcus granulosus infection is high in Iran due to the presence of various intermediate hosts in this country. Considering the rising trend of this zoonotic parasitic disease based on national epidemiological studies, diagnosis is of great significance. WHO has suggested the use of specific antigens, especially antigen B (AgB) for serological diagnostic tests. In general, AgB is a polymeric lipoprotein, which disintegrates into 8.12, 16, and 20.24 kDa subunits. In the present study, we applied three different methods for AgB isolation from hydatid cyst fluid (HCF) and compared their efficacy in AgB isolation. Finally, the protein concentration of this antigen was measured by Bradford assay and confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that the application of polyethylene glycol (PEG 4000) as a thickener agent beside purification of HCF in dialysis bag and filtering and also dialysis against acetate buffer leading to the best quantity in purified antigen B.http://www.archrazi.com/article_106448.htmlAntigen BEchinococcus granulosusHydatid cystIsolation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shirazi, S. Madani, R. Hoghooghi Rad, N. Ranjbar Bahadori, S. |
spellingShingle |
Shirazi, S. Madani, R. Hoghooghi Rad, N. Ranjbar Bahadori, S. Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods Archives of Razi Institute Antigen B Echinococcus granulosus Hydatid cyst Isolation |
author_facet |
Shirazi, S. Madani, R. Hoghooghi Rad, N. Ranjbar Bahadori, S. |
author_sort |
Shirazi, S. |
title |
Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods |
title_short |
Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods |
title_full |
Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods |
title_fullStr |
Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods |
title_full_unstemmed |
Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods |
title_sort |
isolation and purification of echinococcus granulosus antigen b from hydatid cyst fluid using three different methods |
publisher |
Razi Vaccine and Serum Research Institute |
series |
Archives of Razi Institute |
issn |
0365-3439 2008-9872 |
publishDate |
2016-07-01 |
description |
Hydatid cyst, the larval stage of cestodes Echinococcus spp., is recognized as a zoonotic infection in the world. The World Health Organization (WHO) has recently classified echinococcosis in a group of neglected tropical diseases. The prevalence of Echinococcus granulosus infection is high in Iran due to the presence of various intermediate hosts in this country. Considering the rising trend of this zoonotic parasitic disease based on national epidemiological studies, diagnosis is of great significance. WHO has suggested the use of specific
antigens, especially antigen B (AgB) for serological diagnostic tests. In general, AgB is a polymeric lipoprotein, which disintegrates into 8.12, 16, and 20.24 kDa subunits. In the present study, we applied three different methods for AgB isolation from hydatid cyst fluid (HCF) and compared their efficacy in AgB isolation. Finally, the protein concentration of this antigen was measured by Bradford assay and confirmed by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that the application of polyethylene glycol (PEG 4000) as a thickener agent beside purification of HCF in dialysis bag and filtering and also dialysis against acetate buffer leading to the best quantity in purified antigen B. |
topic |
Antigen B Echinococcus granulosus Hydatid cyst Isolation |
url |
http://www.archrazi.com/article_106448.html |
work_keys_str_mv |
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