Large scale loss of data in low-diversity illumina sequencing libraries can be recovered by deferred cluster calling.
Massively parallel DNA sequencing is capable of sequencing tens of millions of DNA fragments at the same time. However, sequence bias in the initial cycles, which are used to determine the coordinates of individual clusters, causes a loss of fidelity in cluster identification on Illumina Genome Anal...
Main Authors: | Felix Krueger, Simon R Andrews, Cameron S Osborne |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3030592?pdf=render |
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