Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

• Main Authors: Yara P. Cid, Thais P. Ferreira, Deborah M. V. C. Medeiros, Rodrigo M. Oliveira, Nathália C. C. Silva, Viviane S. Magalhães, Fábio B. Scott
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2012-01-01
• Series: Química Nova
• Subjects: fipronil; bovine plasma; LC-UV
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001000029&lng=en&tlng=en

A fast and efficient method has been developed and validated for the determination of fipronil in bovine plasma. Samples were subjected to solid-phase extraction (SPE) followed by reversed phase liquid chromatography (LC) separation, using acetonitrile/water (60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet (UV) detection at 210 nm. Ethiprole was used as the internal standard (IS). The method was found to be linear over the range 5-500 ng/mL (r = 0.999). The limit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfully applied to monitor plasma concentrations following subcutaneous administration of fipronil in cattle.