Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences
Lotus (Nelumbo nucifera Gaertn.) is an economically important perennial aquatic herb plant in China. Presently, viral diseases caused by Cucumber mosaic virus (CMV) and Dasheen mosaic virus (DsMV) are detected in lotus. In this study, the putatively novel badnaviruses from lotus plants (LBVs) were i...
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KeAi Communications Co., Ltd.
2020-09-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014120300650 |
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doaj-d23165d6dcfa4138b647c4eacf2873362021-04-02T11:00:13ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412020-09-0165335342Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region SequencesZhen He0Tingting Dong1Tielin Wang2Wen Chen3Xian Liu4Liangjun Li5School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaState Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, China; Corresponding author. Tel: +86 514 87979394Lotus (Nelumbo nucifera Gaertn.) is an economically important perennial aquatic herb plant in China. Presently, viral diseases caused by Cucumber mosaic virus (CMV) and Dasheen mosaic virus (DsMV) are detected in lotus. In this study, the putatively novel badnaviruses from lotus plants (LBVs) were identified using next-generation sequencing of siRNAs and conventional Sanger sequencing based on the RT/RNase H coding region sequences. Lotus plants infected by LBVs harbored virus quasispecies. A unique recombination event of LBVs was found in a single lotus plant. Sap inoculation showed that LBVs could actively replicate in the lotus plants, but without a wide host range. The field survey of 43 lotus plants in Jiangsu Province showed a prevalence of 62.8% for LBVs, confirming that it is widely distributed in Jiangsu Province of China.http://www.sciencedirect.com/science/article/pii/S2468014120300650Nelumbo nuciferaBadnavirusesmixed infectiongenetic variation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Zhen He Tingting Dong Tielin Wang Wen Chen Xian Liu Liangjun Li |
| spellingShingle |
Zhen He Tingting Dong Tielin Wang Wen Chen Xian Liu Liangjun Li Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences Horticultural Plant Journal Nelumbo nucifera Badnaviruses mixed infection genetic variation |
| author_facet |
Zhen He Tingting Dong Tielin Wang Wen Chen Xian Liu Liangjun Li |
| author_sort |
Zhen He |
| title |
Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences |
| title_short |
Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences |
| title_full |
Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences |
| title_fullStr |
Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences |
| title_full_unstemmed |
Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region Sequences |
| title_sort |
genetic variation of the novel badnaviruses infecting nelumbo nucifera based on the rt/rnase h coding region sequences |
| publisher |
KeAi Communications Co., Ltd. |
| series |
Horticultural Plant Journal |
| issn |
2468-0141 |
| publishDate |
2020-09-01 |
| description |
Lotus (Nelumbo nucifera Gaertn.) is an economically important perennial aquatic herb plant in China. Presently, viral diseases caused by Cucumber mosaic virus (CMV) and Dasheen mosaic virus (DsMV) are detected in lotus. In this study, the putatively novel badnaviruses from lotus plants (LBVs) were identified using next-generation sequencing of siRNAs and conventional Sanger sequencing based on the RT/RNase H coding region sequences. Lotus plants infected by LBVs harbored virus quasispecies. A unique recombination event of LBVs was found in a single lotus plant. Sap inoculation showed that LBVs could actively replicate in the lotus plants, but without a wide host range. The field survey of 43 lotus plants in Jiangsu Province showed a prevalence of 62.8% for LBVs, confirming that it is widely distributed in Jiangsu Province of China. |
| topic |
Nelumbo nucifera Badnaviruses mixed infection genetic variation |
| url |
http://www.sciencedirect.com/science/article/pii/S2468014120300650 |
| work_keys_str_mv |
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