Bacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosa
Summary C‐di‐GMP is a key signalling molecule which impacts bacterial motility and biofilm formation and is formed by the condensation of two GTP molecules by a diguanylate cyclase. We here describe the identification and characterization of a family of bacteriophage‐encoded peptides that directly i...
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Series: | Microbial Biotechnology |
Online Access: | https://doi.org/10.1111/1751-7915.13728 |
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doaj-d2323145e57f401286466b86ae9c57912021-04-30T10:22:41ZengWileyMicrobial Biotechnology1751-79152021-05-0114396797810.1111/1751-7915.13728Bacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosaJeroen De Smet0Jeroen Wagemans1Hanne Hendrix2Ines Staes3Annegrete Visnapuu4Benjamin Horemans5Abram Aertsen6Rob Lavigne7Laboratory of Gene Technology Department of Biosystems KU Leuven Heverlee3001BelgiumLaboratory of Gene Technology Department of Biosystems KU Leuven Heverlee3001BelgiumLaboratory of Gene Technology Department of Biosystems KU Leuven Heverlee3001BelgiumLaboratory of Food Microbiology Department of Microbial and Molecular Systems KU Leuven Heverlee3001BelgiumLaboratory of Gene Technology Department of Biosystems KU Leuven Heverlee3001BelgiumDepartment of Earth and Environmental Sciences KU Leuven Heverlee3001BelgiumLaboratory of Food Microbiology Department of Microbial and Molecular Systems KU Leuven Heverlee3001BelgiumLaboratory of Gene Technology Department of Biosystems KU Leuven Heverlee3001BelgiumSummary C‐di‐GMP is a key signalling molecule which impacts bacterial motility and biofilm formation and is formed by the condensation of two GTP molecules by a diguanylate cyclase. We here describe the identification and characterization of a family of bacteriophage‐encoded peptides that directly impact c‐di‐GMP signalling in Pseudomonas aeruginosa. These phage proteins target Pseudomonas diguanylate cyclase YfiN by direct protein interaction (termed YIPs, YfiN Interacting Peptides). YIPs induce an increase of c‐di‐GMP production in the host cell, resulting in a decrease in motility and an increase in biofilm mass in P. aeruginosa. A dynamic analysis of the biofilm morphology indicates a denser biofilm structure after induction of the phage protein. This intracellular signalling interference strategy by a lytic phage constitutes an unexplored phage‐based mechanism of metabolic regulation and could potentially serve as inspiration for the development of molecules that interfere with biofilm formation in P. aeruginosa and other pathogens.https://doi.org/10.1111/1751-7915.13728 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeroen De Smet Jeroen Wagemans Hanne Hendrix Ines Staes Annegrete Visnapuu Benjamin Horemans Abram Aertsen Rob Lavigne |
spellingShingle |
Jeroen De Smet Jeroen Wagemans Hanne Hendrix Ines Staes Annegrete Visnapuu Benjamin Horemans Abram Aertsen Rob Lavigne Bacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosa Microbial Biotechnology |
author_facet |
Jeroen De Smet Jeroen Wagemans Hanne Hendrix Ines Staes Annegrete Visnapuu Benjamin Horemans Abram Aertsen Rob Lavigne |
author_sort |
Jeroen De Smet |
title |
Bacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosa |
title_short |
Bacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosa |
title_full |
Bacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosa |
title_fullStr |
Bacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosa |
title_full_unstemmed |
Bacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosa |
title_sort |
bacteriophage‐mediated interference of the c‐di‐gmp signalling pathway in pseudomonas aeruginosa |
publisher |
Wiley |
series |
Microbial Biotechnology |
issn |
1751-7915 |
publishDate |
2021-05-01 |
description |
Summary C‐di‐GMP is a key signalling molecule which impacts bacterial motility and biofilm formation and is formed by the condensation of two GTP molecules by a diguanylate cyclase. We here describe the identification and characterization of a family of bacteriophage‐encoded peptides that directly impact c‐di‐GMP signalling in Pseudomonas aeruginosa. These phage proteins target Pseudomonas diguanylate cyclase YfiN by direct protein interaction (termed YIPs, YfiN Interacting Peptides). YIPs induce an increase of c‐di‐GMP production in the host cell, resulting in a decrease in motility and an increase in biofilm mass in P. aeruginosa. A dynamic analysis of the biofilm morphology indicates a denser biofilm structure after induction of the phage protein. This intracellular signalling interference strategy by a lytic phage constitutes an unexplored phage‐based mechanism of metabolic regulation and could potentially serve as inspiration for the development of molecules that interfere with biofilm formation in P. aeruginosa and other pathogens. |
url |
https://doi.org/10.1111/1751-7915.13728 |
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