Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci

Four microsatellite loci were used to achieve genetic characterization of six stocks from Litopenaeus vannamei used for aquaculture in Cuba: second generation from first introduction (S2-1), first generation from the second one (S1-2), from the third one (S1-3), and the fourth one (S1-4) and the cro...

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Main Authors: Anna Pérez-Beloborodova, Adriana Artiles-Valor, Lourdes Pérez-Jar, Damir Hernández-Martínez, Missael Guerra-Aznay, Georgina Espinosa-López
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:International Journal of Zoology
Online Access:http://dx.doi.org/10.1155/2012/352165
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spelling doaj-d2378c87a3fb4d249e92e00b6bfceb082020-11-25T00:19:21ZengHindawi LimitedInternational Journal of Zoology1687-84771687-84852012-01-01201210.1155/2012/352165352165Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite LociAnna Pérez-Beloborodova0Adriana Artiles-Valor1Lourdes Pérez-Jar2Damir Hernández-Martínez3Missael Guerra-Aznay4Georgina Espinosa-López5Conservation Genetic Group, Marine Research Centre, University of Havana, Street 16 No 114, Playa Havana, CP 11300, CubaMolecular Biology Laboratory, Aquaculture Division, Fisheries Research Centre, 5th Avenue and 246, Barlovento, Playa, Havana, CP 19100, CubaMolecular Biology Laboratory, Aquaculture Division, Fisheries Research Centre, 5th Avenue and 246, Barlovento, Playa, Havana, CP 19100, CubaConservation Genetic Group, Marine Research Centre, University of Havana, Street 16 No 114, Playa Havana, CP 11300, CubaMolecular Biology Laboratory, Aquaculture Division, Fisheries Research Centre, 5th Avenue and 246, Barlovento, Playa, Havana, CP 19100, CubaDepartment of Biochemistry, Faculty of Biology, Havana University, Street 25 No. 455 between J. and I. Vedado, Havana, CubaFour microsatellite loci were used to achieve genetic characterization of six stocks from Litopenaeus vannamei used for aquaculture in Cuba: second generation from first introduction (S2-1), first generation from the second one (S1-2), from the third one (S1-3), and the fourth one (S1-4) and the crossings from two parental population: first generation from the first with first generation from the third (S1-1 × S1-3) and first generation from the second with first generation from the third (S1-2 × S1-3). 66% (16/24) of genetic systems in total loci were in genetic disequilibrium. The four microsatellite loci were polymorphic for all six stocks. Major quantities of allelic variants correspond to locus Pvan 1758, which is at the same time that one where there are private alleles from first generation of the third. All Fst comparisons were significant. This indicates big differences between stocks. The highest values are those in which there is presence of the second introduction. This introduction and its descendants are also more consanguineous.http://dx.doi.org/10.1155/2012/352165
collection DOAJ
language English
format Article
sources DOAJ
author Anna Pérez-Beloborodova
Adriana Artiles-Valor
Lourdes Pérez-Jar
Damir Hernández-Martínez
Missael Guerra-Aznay
Georgina Espinosa-López
spellingShingle Anna Pérez-Beloborodova
Adriana Artiles-Valor
Lourdes Pérez-Jar
Damir Hernández-Martínez
Missael Guerra-Aznay
Georgina Espinosa-López
Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci
International Journal of Zoology
author_facet Anna Pérez-Beloborodova
Adriana Artiles-Valor
Lourdes Pérez-Jar
Damir Hernández-Martínez
Missael Guerra-Aznay
Georgina Espinosa-López
author_sort Anna Pérez-Beloborodova
title Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci
title_short Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci
title_full Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci
title_fullStr Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci
title_full_unstemmed Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci
title_sort genetic characterization of six stocks of litopenaeus vannamei used in cuba for aquaculture by means of microsatellite loci
publisher Hindawi Limited
series International Journal of Zoology
issn 1687-8477
1687-8485
publishDate 2012-01-01
description Four microsatellite loci were used to achieve genetic characterization of six stocks from Litopenaeus vannamei used for aquaculture in Cuba: second generation from first introduction (S2-1), first generation from the second one (S1-2), from the third one (S1-3), and the fourth one (S1-4) and the crossings from two parental population: first generation from the first with first generation from the third (S1-1 × S1-3) and first generation from the second with first generation from the third (S1-2 × S1-3). 66% (16/24) of genetic systems in total loci were in genetic disequilibrium. The four microsatellite loci were polymorphic for all six stocks. Major quantities of allelic variants correspond to locus Pvan 1758, which is at the same time that one where there are private alleles from first generation of the third. All Fst comparisons were significant. This indicates big differences between stocks. The highest values are those in which there is presence of the second introduction. This introduction and its descendants are also more consanguineous.
url http://dx.doi.org/10.1155/2012/352165
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