Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study.
Phytosterols, besides hypocholesterolemic effect, present anti-inflammatory properties. Little information is available about their efficacy in Inflammatory Bowel Disease (IBD). Therefore, we have evaluated the effect of a mixture of phytosterols on prevention/induction/remission in a murine experim...
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doaj-d23ca394d27c498ebba5c19ddb78ebc72020-11-25T00:47:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10811210.1371/journal.pone.0108112Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study.Rita AldiniMatteo MicucciMonica CeveniniRomana FatoChristian BergaminiCristina NanniMassimiliano ContCecilia CamborataSilvia SpinozziMarco MontagnaniGiulia RodaAntonia D'Errico-GrigioniFrancesca RosiniAldo RodaGiuseppe MazzellaAlberto ChiariniRoberta BudriesiPhytosterols, besides hypocholesterolemic effect, present anti-inflammatory properties. Little information is available about their efficacy in Inflammatory Bowel Disease (IBD). Therefore, we have evaluated the effect of a mixture of phytosterols on prevention/induction/remission in a murine experimental model of colitis. Phytosterols were administered x os before, during and after colitis induction with Dextran Sodium Sulfate (DSS) in mice. Disease Activity Index (DAI), colon length, histopathology score, 18F-FDG microPET, oxidative stress in the intestinal tissue (ileum and colon) and gallbladder ileum and colon spontaneous and carbachol (CCh) induced motility, plasma lipids and plasma, liver and biliary bile acids (BA) were evaluated. A similar longitudinal study was performed in a DSS colitis control group. Mice treated with DSS developed severe colitis as shown by DAI, colon length, histopathology score, 18F-FDG microPET, oxidative stress. Both spontaneous and induced ileal and colonic motility were severely disturbed. The same was observed with gallbladder. DSS colitis resulted in an increase in plasma cholesterol, and a modification of the BA pattern. Phytosterols feeding did not prevent colitis onset but significantly reduced the severity of the disease and improved clinical and histological remission. It had strong antioxidant effects, almost restored colon, ileal and gallbladder motility. Plasmatic levels of cholesterol were also reduced. DSS induced a modification in the BA pattern consistent with an increase in the intestinal BA deconjugating bacteria, prevented by phytosterols. Phytosterols seem a potential nutraceutical tool for gastrointestinal inflammatory diseases, combining metabolic systematic and local anti-inflammatory effects.http://europepmc.org/articles/PMC4182327?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rita Aldini Matteo Micucci Monica Cevenini Romana Fato Christian Bergamini Cristina Nanni Massimiliano Cont Cecilia Camborata Silvia Spinozzi Marco Montagnani Giulia Roda Antonia D'Errico-Grigioni Francesca Rosini Aldo Roda Giuseppe Mazzella Alberto Chiarini Roberta Budriesi |
spellingShingle |
Rita Aldini Matteo Micucci Monica Cevenini Romana Fato Christian Bergamini Cristina Nanni Massimiliano Cont Cecilia Camborata Silvia Spinozzi Marco Montagnani Giulia Roda Antonia D'Errico-Grigioni Francesca Rosini Aldo Roda Giuseppe Mazzella Alberto Chiarini Roberta Budriesi Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study. PLoS ONE |
author_facet |
Rita Aldini Matteo Micucci Monica Cevenini Romana Fato Christian Bergamini Cristina Nanni Massimiliano Cont Cecilia Camborata Silvia Spinozzi Marco Montagnani Giulia Roda Antonia D'Errico-Grigioni Francesca Rosini Aldo Roda Giuseppe Mazzella Alberto Chiarini Roberta Budriesi |
author_sort |
Rita Aldini |
title |
Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study. |
title_short |
Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study. |
title_full |
Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study. |
title_fullStr |
Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study. |
title_full_unstemmed |
Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study. |
title_sort |
antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Phytosterols, besides hypocholesterolemic effect, present anti-inflammatory properties. Little information is available about their efficacy in Inflammatory Bowel Disease (IBD). Therefore, we have evaluated the effect of a mixture of phytosterols on prevention/induction/remission in a murine experimental model of colitis. Phytosterols were administered x os before, during and after colitis induction with Dextran Sodium Sulfate (DSS) in mice. Disease Activity Index (DAI), colon length, histopathology score, 18F-FDG microPET, oxidative stress in the intestinal tissue (ileum and colon) and gallbladder ileum and colon spontaneous and carbachol (CCh) induced motility, plasma lipids and plasma, liver and biliary bile acids (BA) were evaluated. A similar longitudinal study was performed in a DSS colitis control group. Mice treated with DSS developed severe colitis as shown by DAI, colon length, histopathology score, 18F-FDG microPET, oxidative stress. Both spontaneous and induced ileal and colonic motility were severely disturbed. The same was observed with gallbladder. DSS colitis resulted in an increase in plasma cholesterol, and a modification of the BA pattern. Phytosterols feeding did not prevent colitis onset but significantly reduced the severity of the disease and improved clinical and histological remission. It had strong antioxidant effects, almost restored colon, ileal and gallbladder motility. Plasmatic levels of cholesterol were also reduced. DSS induced a modification in the BA pattern consistent with an increase in the intestinal BA deconjugating bacteria, prevented by phytosterols. Phytosterols seem a potential nutraceutical tool for gastrointestinal inflammatory diseases, combining metabolic systematic and local anti-inflammatory effects. |
url |
http://europepmc.org/articles/PMC4182327?pdf=render |
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