Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis
Objective: To obtain the effective method of ovarian function preservation with granulose cell apoptosis assessment. Ovarian tissue vitrification became a method for ovarian function preservation in women with cancer. This technique can be done anytime without delay on cancer therapy both in pre...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Indonesian Society of Obstetrics and Gynecology
2016-04-01
|
Series: | Indonesian Journal of Obstetrics and Gynecology |
Online Access: | http://inajog.com/index.php/journal/article/view/81 |
id |
doaj-d24625b2fd1d4c6fba04c6097402b2e7 |
---|---|
record_format |
Article |
spelling |
doaj-d24625b2fd1d4c6fba04c6097402b2e72021-06-02T04:18:20ZengIndonesian Society of Obstetrics and GynecologyIndonesian Journal of Obstetrics and Gynecology2338-64012338-73352016-04-01889210.32771/inajog.v4i2.8181Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell ApoptosisBudi Wiweko0Huthia Andriyana1Achmad Aulia2Faculty of Medicine University of Indonesia/ Dr. Cipto Mangunkusumo General Hospital JakartaFaculty of Medicine University of Indonesia/ Dr. Cipto Mangunkusumo General Hospital JakartaFaculty of Medicine University of Indonesia/ Dr. Cipto Mangunkusumo General Hospital JakartaObjective: To obtain the effective method of ovarian function preservation with granulose cell apoptosis assessment. Ovarian tissue vitrification became a method for ovarian function preservation in women with cancer. This technique can be done anytime without delay on cancer therapy both in prepubertal and unmarried patient. It can also store many primordial follicles. Ovarian tissue vitrification study is still limited to animal test and there are no data about apoptosis assessment after ovarian vitrification in human ovary. Method: This quasi experimental study was held in Department of Obstetrics and Gynecology Faculty of Medicine University of Indonesia - Dr. Cipto Mangunkusumo General Hospital and Fatmawati Hospital Jakarta from March 2012 to May 2015. Ovaries from thirteen women between 31 and 37 years old who underwent oophorectomy with gynecological indication were examined. Result: There was no morphological difference between follicles from fresh and warmed-vitrified ovaries. The mean protein Bax expression on the fresh ovaries assessed in the form of H-score was 1.66 (SD 0.14) compared with 1.68 (SD 0.13) on the warmedvitrified group (p=0.165). The mean protein Bcl-2 expression on the fresh ovaries examined in the form of H-score was 1.73 (SD 0.10) compared with 1.71 (SD 0.10) on the warmed-vitrified group (p=0.068). Conclusion: Ovarian tissue vitrification does not affect the Bax and Bcl-2 expression on human ovary. [Indones J Obstet Gynecol 2016; 4-2: 88-92] Keywords: apoptosis, bax, Bcl-2, ovarian tissue vitrificationhttp://inajog.com/index.php/journal/article/view/81 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Budi Wiweko Huthia Andriyana Achmad Aulia |
spellingShingle |
Budi Wiweko Huthia Andriyana Achmad Aulia Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis Indonesian Journal of Obstetrics and Gynecology |
author_facet |
Budi Wiweko Huthia Andriyana Achmad Aulia |
author_sort |
Budi Wiweko |
title |
Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis |
title_short |
Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis |
title_full |
Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis |
title_fullStr |
Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis |
title_full_unstemmed |
Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis |
title_sort |
ovarian tissue vitrification as a method for ovarian preservation in women with cancer: an analysis of granulose cell apoptosis |
publisher |
Indonesian Society of Obstetrics and Gynecology |
series |
Indonesian Journal of Obstetrics and Gynecology |
issn |
2338-6401 2338-7335 |
publishDate |
2016-04-01 |
description |
Objective: To obtain the effective method of ovarian function preservation
with granulose cell apoptosis assessment. Ovarian tissue
vitrification became a method for ovarian function preservation in
women with cancer. This technique can be done anytime without
delay on cancer therapy both in prepubertal and unmarried patient.
It can also store many primordial follicles. Ovarian tissue vitrification
study is still limited to animal test and there are no data about apoptosis
assessment after ovarian vitrification in human ovary.
Method: This quasi experimental study was held in Department of
Obstetrics and Gynecology Faculty of Medicine University of Indonesia
- Dr. Cipto Mangunkusumo General Hospital and Fatmawati
Hospital Jakarta from March 2012 to May 2015. Ovaries from thirteen
women between 31 and 37 years old who underwent oophorectomy
with gynecological indication were examined.
Result: There was no morphological difference between follicles
from fresh and warmed-vitrified ovaries. The mean protein Bax
expression on the fresh ovaries assessed in the form of H-score
was 1.66 (SD 0.14) compared with 1.68 (SD 0.13) on the warmedvitrified
group (p=0.165). The mean protein Bcl-2 expression on the
fresh ovaries examined in the form of H-score was 1.73 (SD 0.10)
compared with 1.71 (SD 0.10) on the warmed-vitrified group
(p=0.068).
Conclusion: Ovarian tissue vitrification does not affect the Bax and
Bcl-2 expression on human ovary.
[Indones J Obstet Gynecol 2016; 4-2: 88-92]
Keywords: apoptosis, bax, Bcl-2, ovarian tissue vitrification |
url |
http://inajog.com/index.php/journal/article/view/81 |
work_keys_str_mv |
AT budiwiweko ovariantissuevitrificationasamethodforovarianpreservationinwomenwithcancerananalysisofgranulosecellapoptosis AT huthiaandriyana ovariantissuevitrificationasamethodforovarianpreservationinwomenwithcancerananalysisofgranulosecellapoptosis AT achmadaulia ovariantissuevitrificationasamethodforovarianpreservationinwomenwithcancerananalysisofgranulosecellapoptosis |
_version_ |
1721408569341902848 |