The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation.
Staphylococcus epidermidis is the most common cause of device-associated infections. It has been shown that active and passive immunization in an animal model against protein SesC significantly reduces S. epidermidis biofilm-associated infections. In order to elucidate its role, knock-out of sesC or...
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doaj-d2467d0a15da4504ac545b329ff7b19f2020-11-25T01:52:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014670410.1371/journal.pone.0146704The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation.Laleh KhodaparastLadan KhodaparastMohammad ShahrooeiBenoit StijlemansRita MerckxPieter BaatsenJames P O'GaraElaine WatersLieve Van MellaertJohan Van EldereStaphylococcus epidermidis is the most common cause of device-associated infections. It has been shown that active and passive immunization in an animal model against protein SesC significantly reduces S. epidermidis biofilm-associated infections. In order to elucidate its role, knock-out of sesC or isolation of S. epidermidis sesC-negative mutants were attempted, however, without success. As an alternative strategy, sesC was introduced into Staphylococcus aureus 8325-4 and its isogenic icaADBC and srtA mutants, into the clinical methicillin-sensitive S. aureus isolate MSSA4 and the MRSA S. aureus isolate BH1CC, which all lack sesC. Transformation of these strains with sesC i) changed the biofilm phenotype of strains 8325-4 and MSSA4 from PIA-dependent to proteinaceous even though PIA synthesis was not affected, ii) converted the non-biofilm-forming strain 8325-4 ica::tet to a proteinaceous biofilm-forming strain, iii) impaired PIA-dependent biofilm formation by 8325-4 srtA::tet, iv) had no impact on protein-mediated biofilm formation of BH1CC and v) increased in vivo catheter and organ colonization by strain 8325-4. Furthermore, treatment with anti-SesC antibodies significantly reduced in vitro biofilm formation and in vivo colonization by these transformants expressing sesC. These findings strongly suggest that SesC is involved in S. epidermidis attachment to and subsequent biofilm formation on a substrate.http://europepmc.org/articles/PMC4723045?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laleh Khodaparast Ladan Khodaparast Mohammad Shahrooei Benoit Stijlemans Rita Merckx Pieter Baatsen James P O'Gara Elaine Waters Lieve Van Mellaert Johan Van Eldere |
spellingShingle |
Laleh Khodaparast Ladan Khodaparast Mohammad Shahrooei Benoit Stijlemans Rita Merckx Pieter Baatsen James P O'Gara Elaine Waters Lieve Van Mellaert Johan Van Eldere The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation. PLoS ONE |
author_facet |
Laleh Khodaparast Ladan Khodaparast Mohammad Shahrooei Benoit Stijlemans Rita Merckx Pieter Baatsen James P O'Gara Elaine Waters Lieve Van Mellaert Johan Van Eldere |
author_sort |
Laleh Khodaparast |
title |
The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation. |
title_short |
The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation. |
title_full |
The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation. |
title_fullStr |
The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation. |
title_full_unstemmed |
The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation. |
title_sort |
possible role of staphylococcus epidermidis lpxtg surface protein sesc in biofilm formation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Staphylococcus epidermidis is the most common cause of device-associated infections. It has been shown that active and passive immunization in an animal model against protein SesC significantly reduces S. epidermidis biofilm-associated infections. In order to elucidate its role, knock-out of sesC or isolation of S. epidermidis sesC-negative mutants were attempted, however, without success. As an alternative strategy, sesC was introduced into Staphylococcus aureus 8325-4 and its isogenic icaADBC and srtA mutants, into the clinical methicillin-sensitive S. aureus isolate MSSA4 and the MRSA S. aureus isolate BH1CC, which all lack sesC. Transformation of these strains with sesC i) changed the biofilm phenotype of strains 8325-4 and MSSA4 from PIA-dependent to proteinaceous even though PIA synthesis was not affected, ii) converted the non-biofilm-forming strain 8325-4 ica::tet to a proteinaceous biofilm-forming strain, iii) impaired PIA-dependent biofilm formation by 8325-4 srtA::tet, iv) had no impact on protein-mediated biofilm formation of BH1CC and v) increased in vivo catheter and organ colonization by strain 8325-4. Furthermore, treatment with anti-SesC antibodies significantly reduced in vitro biofilm formation and in vivo colonization by these transformants expressing sesC. These findings strongly suggest that SesC is involved in S. epidermidis attachment to and subsequent biofilm formation on a substrate. |
url |
http://europepmc.org/articles/PMC4723045?pdf=render |
work_keys_str_mv |
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