Fluoride-tolerant mutants of Aspergillus niger show enhanced phosphate solubilization capacity.
P-solubilizing microorganisms are a promising alternative for a sustainable use of P against a backdrop of depletion of high-grade rock phosphates (RPs). Nevertheless, toxic elements present in RPs, such as fluorine, can negatively affect microbial solubilization. Thus, this study aimed at selecting...
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doaj-d262d78ca1884de7aa96a441ca2b794b2020-11-25T02:47:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11024610.1371/journal.pone.0110246Fluoride-tolerant mutants of Aspergillus niger show enhanced phosphate solubilization capacity.Ubiana de Cássia SilvaGilberto de Oliveira MendesNina Morena R M SilvaJosiane Leal DuarteIvo Ribeiro SilvaMarcos Rogério TótolaMaurício Dutra CostaP-solubilizing microorganisms are a promising alternative for a sustainable use of P against a backdrop of depletion of high-grade rock phosphates (RPs). Nevertheless, toxic elements present in RPs, such as fluorine, can negatively affect microbial solubilization. Thus, this study aimed at selecting Aspergillus niger mutants efficient at P solubilization in the presence of fluoride (F-). The mutants were obtained by exposition of conidia to UV light followed by screening in a medium supplemented with Ca3(PO4)2 and F-. The mutant FS1-555 showed the highest solubilization in the presence of F-, releasing approximately 70% of the P contained in Ca3(PO4)2, a value 1.7 times higher than that obtained for the wild type (WT). The mutant FS1-331 showed improved ability of solubilizing fluorapatites, increasing the solubilization of Araxá, Catalão, and Patos RPs by 1.7, 1.6, and 2.5 times that of the WT, respectively. These mutants also grew better in the presence of F-, indicating that mutagenesis allowed the acquisition of F- tolerance. Higher production of oxalic acid by FS1-331 correlated with its improved capacity for RP solubilization. This mutant represents a significant improvement and possess a high potential for application in solubilization systems with fluoride-rich phosphate sources.http://europepmc.org/articles/PMC4195724?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ubiana de Cássia Silva Gilberto de Oliveira Mendes Nina Morena R M Silva Josiane Leal Duarte Ivo Ribeiro Silva Marcos Rogério Tótola Maurício Dutra Costa |
spellingShingle |
Ubiana de Cássia Silva Gilberto de Oliveira Mendes Nina Morena R M Silva Josiane Leal Duarte Ivo Ribeiro Silva Marcos Rogério Tótola Maurício Dutra Costa Fluoride-tolerant mutants of Aspergillus niger show enhanced phosphate solubilization capacity. PLoS ONE |
author_facet |
Ubiana de Cássia Silva Gilberto de Oliveira Mendes Nina Morena R M Silva Josiane Leal Duarte Ivo Ribeiro Silva Marcos Rogério Tótola Maurício Dutra Costa |
author_sort |
Ubiana de Cássia Silva |
title |
Fluoride-tolerant mutants of Aspergillus niger show enhanced phosphate solubilization capacity. |
title_short |
Fluoride-tolerant mutants of Aspergillus niger show enhanced phosphate solubilization capacity. |
title_full |
Fluoride-tolerant mutants of Aspergillus niger show enhanced phosphate solubilization capacity. |
title_fullStr |
Fluoride-tolerant mutants of Aspergillus niger show enhanced phosphate solubilization capacity. |
title_full_unstemmed |
Fluoride-tolerant mutants of Aspergillus niger show enhanced phosphate solubilization capacity. |
title_sort |
fluoride-tolerant mutants of aspergillus niger show enhanced phosphate solubilization capacity. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
P-solubilizing microorganisms are a promising alternative for a sustainable use of P against a backdrop of depletion of high-grade rock phosphates (RPs). Nevertheless, toxic elements present in RPs, such as fluorine, can negatively affect microbial solubilization. Thus, this study aimed at selecting Aspergillus niger mutants efficient at P solubilization in the presence of fluoride (F-). The mutants were obtained by exposition of conidia to UV light followed by screening in a medium supplemented with Ca3(PO4)2 and F-. The mutant FS1-555 showed the highest solubilization in the presence of F-, releasing approximately 70% of the P contained in Ca3(PO4)2, a value 1.7 times higher than that obtained for the wild type (WT). The mutant FS1-331 showed improved ability of solubilizing fluorapatites, increasing the solubilization of Araxá, Catalão, and Patos RPs by 1.7, 1.6, and 2.5 times that of the WT, respectively. These mutants also grew better in the presence of F-, indicating that mutagenesis allowed the acquisition of F- tolerance. Higher production of oxalic acid by FS1-331 correlated with its improved capacity for RP solubilization. This mutant represents a significant improvement and possess a high potential for application in solubilization systems with fluoride-rich phosphate sources. |
url |
http://europepmc.org/articles/PMC4195724?pdf=render |
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