Genotoxicity following Organophosphate Pesticides Exposure among Orang Asli Children Living in an Agricultural Island in Kuala Langat, Selangor, Malaysia
Background: Agriculture is an important sector for the Malaysian economy. The use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. Children living near agricultural fields are at risk of pesticide poisoning. Objective: To evaluate the genotoxic...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
National Iranian Oil Company (NIOC) Health Organization
2016-01-01
|
Series: | The International Journal of Occupational and Environmental Medicine |
Subjects: | |
Online Access: | http://www.theijoem.com/ijoem/index.php/ijoem/article/view/705 |
id |
doaj-d2634c5fe1d547f7a2ddc1e865df76b5 |
---|---|
record_format |
Article |
spelling |
doaj-d2634c5fe1d547f7a2ddc1e865df76b52020-11-24T20:58:46ZengNational Iranian Oil Company (NIOC) Health OrganizationThe International Journal of Occupational and Environmental Medicine2008-65202008-68142016-01-0171425110.15171/ijoem.2016.705234Genotoxicity following Organophosphate Pesticides Exposure among Orang Asli Children Living in an Agricultural Island in Kuala Langat, Selangor, MalaysiaJM Sutris0V How1SA Sumeri2M Muhammad3D Sardi4MT Mohd Mokhtar5H Muhammad6HF Ghazi7ZM Isa8Department of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, MalaysiaDepartment of Environmental and Occupational Health, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, MalaysiaDepartment of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, MalaysiaDepartment of Laboratory Diagnostic Services, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, MalaysiaDepartment of Laboratory Diagnostic Services, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, MalaysiaDepartment of Laboratory Diagnostic Services, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, MalaysiaDepartment of Laboratory Diagnostic Services, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, MalaysiaCommunity Medicine Unit, International Medical School, Management and Science University, Selangor, MalaysiaDepartment of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, MalaysiaBackground: Agriculture is an important sector for the Malaysian economy. The use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. Children living near agricultural fields are at risk of pesticide poisoning. Objective: To evaluate the genotoxic risk among children who exposed to pesticides and measure DNA damage due to pesticides exposure. Methods: In a cross-sectional study 180 Orang Asli Mah Meri children aged between 7 and 12 years were studied. They were all living in an agricultural island in Kuala Langat, Selangor, Malaysia. The data for this study were collected via modified validated questionnaire and food frequency questionnaire, which consisted of 131 food items. 6 urinary organophosphate metabolites were used as biomarkers for pesticides exposure. For genotoxic risk or genetic damage assessment, the level of DNA damage from exfoliated buccal mucosa cells was measured using the comet assay electrophoresis method. Results: Out of 180 respondents, 84 (46.7%) showed positive traces of organophosphate metabolites in their urine. Children with detectable urinary pesticide had a longer tail length (median 43.5; IQR 30.9 to 68.1 μm) than those with undetectable urinary pesticides (median 24.7; IQR 9.5 to 48.1 μm). There was a significant association between the extent of DNA damage and the children's age, length of residence in the area, pesticides detection, and frequency of apple consumption. Conclusion: The organophosphate genotoxicity among children is associated with the amount of exposure (detectability of urinary pesticide) and length of residence in (exposure) the study area.http://www.theijoem.com/ijoem/index.php/ijoem/article/view/705OrganophosphatesPesticidesOrganophosphate poisoningMutagenicity testsComet assay, ChildMalaysia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
JM Sutris V How SA Sumeri M Muhammad D Sardi MT Mohd Mokhtar H Muhammad HF Ghazi ZM Isa |
spellingShingle |
JM Sutris V How SA Sumeri M Muhammad D Sardi MT Mohd Mokhtar H Muhammad HF Ghazi ZM Isa Genotoxicity following Organophosphate Pesticides Exposure among Orang Asli Children Living in an Agricultural Island in Kuala Langat, Selangor, Malaysia The International Journal of Occupational and Environmental Medicine Organophosphates Pesticides Organophosphate poisoning Mutagenicity tests Comet assay, Child Malaysia |
author_facet |
JM Sutris V How SA Sumeri M Muhammad D Sardi MT Mohd Mokhtar H Muhammad HF Ghazi ZM Isa |
author_sort |
JM Sutris |
title |
Genotoxicity following Organophosphate Pesticides Exposure among Orang Asli Children Living in an Agricultural Island in Kuala Langat, Selangor, Malaysia |
title_short |
Genotoxicity following Organophosphate Pesticides Exposure among Orang Asli Children Living in an Agricultural Island in Kuala Langat, Selangor, Malaysia |
title_full |
Genotoxicity following Organophosphate Pesticides Exposure among Orang Asli Children Living in an Agricultural Island in Kuala Langat, Selangor, Malaysia |
title_fullStr |
Genotoxicity following Organophosphate Pesticides Exposure among Orang Asli Children Living in an Agricultural Island in Kuala Langat, Selangor, Malaysia |
title_full_unstemmed |
Genotoxicity following Organophosphate Pesticides Exposure among Orang Asli Children Living in an Agricultural Island in Kuala Langat, Selangor, Malaysia |
title_sort |
genotoxicity following organophosphate pesticides exposure among orang asli children living in an agricultural island in kuala langat, selangor, malaysia |
publisher |
National Iranian Oil Company (NIOC) Health Organization |
series |
The International Journal of Occupational and Environmental Medicine |
issn |
2008-6520 2008-6814 |
publishDate |
2016-01-01 |
description |
Background: Agriculture is an important sector for the Malaysian economy. The use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. Children living near agricultural fields are at risk of pesticide poisoning.
Objective: To evaluate the genotoxic risk among children who exposed to pesticides and measure DNA damage due to pesticides exposure.
Methods: In a cross-sectional study 180 Orang Asli Mah Meri children aged between 7 and 12 years were studied. They were all living in an agricultural island in Kuala Langat, Selangor, Malaysia. The data for this study were collected via modified validated questionnaire and food frequency questionnaire, which consisted of 131 food items. 6 urinary organophosphate metabolites were used as biomarkers for pesticides exposure. For genotoxic risk or genetic damage assessment, the level of DNA damage from exfoliated buccal mucosa cells was measured using the comet assay electrophoresis method.
Results: Out of 180 respondents, 84 (46.7%) showed positive traces of organophosphate metabolites in their urine. Children with detectable urinary pesticide had a longer tail length (median 43.5; IQR 30.9 to 68.1 μm) than those with undetectable urinary pesticides (median 24.7; IQR 9.5 to 48.1 μm). There was a significant association between the extent of DNA damage and the children's age, length of residence in the area, pesticides detection, and frequency of apple consumption.
Conclusion: The organophosphate genotoxicity among children is associated with the amount of exposure (detectability of urinary pesticide) and length of residence in (exposure) the study area. |
topic |
Organophosphates Pesticides Organophosphate poisoning Mutagenicity tests Comet assay, Child Malaysia |
url |
http://www.theijoem.com/ijoem/index.php/ijoem/article/view/705 |
work_keys_str_mv |
AT jmsutris genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia AT vhow genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia AT sasumeri genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia AT mmuhammad genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia AT dsardi genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia AT mtmohdmokhtar genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia AT hmuhammad genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia AT hfghazi genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia AT zmisa genotoxicityfollowingorganophosphatepesticidesexposureamongorangaslichildrenlivinginanagriculturalislandinkualalangatselangormalaysia |
_version_ |
1716784628933591040 |