Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice
<strong>Background and objectives:</strong><em> Tragopogon graminifolius</em> DC. is widely used in Iranian traditional medicine as a gastro-protective and wound healer agent. In the present study the hepatoprotective property of <em>T. graminifolius</em> DC. aqu...
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doaj-d267f19a970647ad8fa2ec1c794202332020-11-25T01:33:18ZengIranian Society of PharmacognosyResearch Journal of Pharmacognosy2345-44582345-59772019-10-0164616910.22127/rjp.2019.9352593525Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in MiceMohammad Mahdi Zangeneh*0Akram Zangeneh1Reza Tahvilian2Rohallah Moradi3Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.<strong>Background and objectives:</strong><em> Tragopogon graminifolius</em> DC. is widely used in Iranian traditional medicine as a gastro-protective and wound healer agent. In the present study the hepatoprotective property of <em>T. graminifolius</em> DC. aqueous extract (TGAE) was investigated against carbon tetrachloride (CCl<sub>4</sub>) induced hepatocellular injury in mice. <strong>Methods:</strong> Fifty male mice were divided into five groups (n=10). Group 1 (control) received 1 ml/kg olive oil intraperitoneally (i.p.) and distilled water orally; Group 2 (untreated) received CCl<sub>4</sub> (50% in olive oil, 1 ml/kg; i.p.); Groups 3, 4 and 5 received CCl<sub>4</sub> and 30, 90 and 270 mg/kg of TGAE (TGAE30, TGAE90 and TGAE270), respectively. At the end of the 45-day treatment, the mice of all groups were euthanized, then blood and liver samples were collected for biochemical and histological parameters analysis. The data was analyzed by one way variance analysis and Duncan’s test using SPSS 21. <strong>Results:</strong> Different doses of TGAE (especially TGAE270) could significantly (p<em>≤</em>0.05) reduce the raised levels of ALP, AST, ALT, Cholesterol, LDL and increased HDL, SOD, CAT as compared to the untreated group. The weight and volume of the hepatic structures decreased significantly (p≤0.05) in several doses of TGAE (especially TGAE270) when compared to the untreated group. <strong>Conclusion:</strong> The results of the present study showed that under the present experimental conditions, TGAE indicated hepatoprotective abilities against CCl<sub>4</sub> induced liver damage in mice.http://www.rjpharmacognosy.ir/article_93525_9d4089f86f4c55afd6815c3448f380d8.pdfaqueous extractCCl4hepatoprotective activityTragopogon graminifolius |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammad Mahdi Zangeneh* Akram Zangeneh Reza Tahvilian Rohallah Moradi |
spellingShingle |
Mohammad Mahdi Zangeneh* Akram Zangeneh Reza Tahvilian Rohallah Moradi Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice Research Journal of Pharmacognosy aqueous extract CCl4 hepatoprotective activity Tragopogon graminifolius |
author_facet |
Mohammad Mahdi Zangeneh* Akram Zangeneh Reza Tahvilian Rohallah Moradi |
author_sort |
Mohammad Mahdi Zangeneh* |
title |
Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice |
title_short |
Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice |
title_full |
Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice |
title_fullStr |
Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice |
title_full_unstemmed |
Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice |
title_sort |
assessment of the hepatoprotective activity of tragopogon graminifolius dc. aqueous extract on ccl4- induced hepatotoxicity in mice |
publisher |
Iranian Society of Pharmacognosy |
series |
Research Journal of Pharmacognosy |
issn |
2345-4458 2345-5977 |
publishDate |
2019-10-01 |
description |
<strong>Background and objectives:</strong><em> Tragopogon graminifolius</em> DC. is widely used in Iranian traditional medicine as a gastro-protective and wound healer agent. In the present study the hepatoprotective property of <em>T. graminifolius</em> DC. aqueous extract (TGAE) was investigated against carbon tetrachloride (CCl<sub>4</sub>) induced hepatocellular injury in mice. <strong>Methods:</strong> Fifty male mice were divided into five groups (n=10). Group 1 (control) received 1 ml/kg olive oil intraperitoneally (i.p.) and distilled water orally; Group 2 (untreated) received CCl<sub>4</sub> (50% in olive oil, 1 ml/kg; i.p.); Groups 3, 4 and 5 received CCl<sub>4</sub> and 30, 90 and 270 mg/kg of TGAE (TGAE30, TGAE90 and TGAE270), respectively. At the end of the 45-day treatment, the mice of all groups were euthanized, then blood and liver samples were collected for biochemical and histological parameters analysis. The data was analyzed by one way variance analysis and Duncan’s test using SPSS 21. <strong>Results:</strong> Different doses of TGAE (especially TGAE270) could significantly (p<em>≤</em>0.05) reduce the raised levels of ALP, AST, ALT, Cholesterol, LDL and increased HDL, SOD, CAT as compared to the untreated group. The weight and volume of the hepatic structures decreased significantly (p≤0.05) in several doses of TGAE (especially TGAE270) when compared to the untreated group. <strong>Conclusion:</strong> The results of the present study showed that under the present experimental conditions, TGAE indicated hepatoprotective abilities against CCl<sub>4</sub> induced liver damage in mice. |
topic |
aqueous extract CCl4 hepatoprotective activity Tragopogon graminifolius |
url |
http://www.rjpharmacognosy.ir/article_93525_9d4089f86f4c55afd6815c3448f380d8.pdf |
work_keys_str_mv |
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