Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study.
Sentinel node navigation surgery is gaining popularity in oral cancer. We assessed application of sentinel lymph node navigation surgery to pharyngeal and laryngeal cancers by evaluating the combination of contrast-enhanced ultrasonography and indocyanine green fluorescence in animal models.This was...
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doaj-d276b85e3f9a48749f463956eb5037df2020-11-24T21:24:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013251110.1371/journal.pone.0132511Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study.Yasunao KogashiwaHiroyuki SakuraiYoshihiro AkimotoDai SatoTetsuya IkedaYoshifumi MatsumotoYorihisa MoroToru KimuraYasuhiro HamanoueTakehiro NakamuraKoichi YamauchiKoichiro SaitoMasashi SugasawaNaoyuki KohnoSentinel node navigation surgery is gaining popularity in oral cancer. We assessed application of sentinel lymph node navigation surgery to pharyngeal and laryngeal cancers by evaluating the combination of contrast-enhanced ultrasonography and indocyanine green fluorescence in animal models.This was a prospective, nonrandomized, experimental study in rabbit and swine animal models. A mixture of indocyanine green and Sonazoid was used as the tracer. The tracer mixture was injected into the tongue, larynx, or pharynx. The sentinel lymph nodes were identified transcutaneously by infra-red camera and contrast-enhanced ultrasonography. Detection time and extraction time of the sentinel lymph nodes were measured. The safety of the tracer mixture in terms of mucosal reaction was evaluated macroscopically and microscopically.Sentinel lymph nodes were detected transcutaneously by contrast-enhanced ultrasonography alone. The number of sentinel lymph nodes detected was one or two. Despite observation of contrast enhancement of Sonazoid for at least 90 minutes, the number of sentinel lymph nodes detected did not change. The average extraction time of sentinel lymph nodes was 4.8 minutes. Indocyanine green fluorescence offered visual information during lymph node biopsy. The safety of the tracer was confirmed by absence of laryngeal edema both macro and microscopically.The combination method of indocyanine green fluorescence and contrast-enhanced ultrasonography for detecting sentinel lymph nodes during surgery for head and neck cancer seems promising, especially for pharyngeal and laryngeal cancer. Further clinical studies to confirm this are warranted.http://europepmc.org/articles/PMC4498775?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yasunao Kogashiwa Hiroyuki Sakurai Yoshihiro Akimoto Dai Sato Tetsuya Ikeda Yoshifumi Matsumoto Yorihisa Moro Toru Kimura Yasuhiro Hamanoue Takehiro Nakamura Koichi Yamauchi Koichiro Saito Masashi Sugasawa Naoyuki Kohno |
spellingShingle |
Yasunao Kogashiwa Hiroyuki Sakurai Yoshihiro Akimoto Dai Sato Tetsuya Ikeda Yoshifumi Matsumoto Yorihisa Moro Toru Kimura Yasuhiro Hamanoue Takehiro Nakamura Koichi Yamauchi Koichiro Saito Masashi Sugasawa Naoyuki Kohno Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study. PLoS ONE |
author_facet |
Yasunao Kogashiwa Hiroyuki Sakurai Yoshihiro Akimoto Dai Sato Tetsuya Ikeda Yoshifumi Matsumoto Yorihisa Moro Toru Kimura Yasuhiro Hamanoue Takehiro Nakamura Koichi Yamauchi Koichiro Saito Masashi Sugasawa Naoyuki Kohno |
author_sort |
Yasunao Kogashiwa |
title |
Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study. |
title_short |
Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study. |
title_full |
Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study. |
title_fullStr |
Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study. |
title_full_unstemmed |
Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study. |
title_sort |
sentinel node biopsy for the head and neck using contrast-enhanced ultrasonography combined with indocyanine green fluorescence in animal models: a feasibility study. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Sentinel node navigation surgery is gaining popularity in oral cancer. We assessed application of sentinel lymph node navigation surgery to pharyngeal and laryngeal cancers by evaluating the combination of contrast-enhanced ultrasonography and indocyanine green fluorescence in animal models.This was a prospective, nonrandomized, experimental study in rabbit and swine animal models. A mixture of indocyanine green and Sonazoid was used as the tracer. The tracer mixture was injected into the tongue, larynx, or pharynx. The sentinel lymph nodes were identified transcutaneously by infra-red camera and contrast-enhanced ultrasonography. Detection time and extraction time of the sentinel lymph nodes were measured. The safety of the tracer mixture in terms of mucosal reaction was evaluated macroscopically and microscopically.Sentinel lymph nodes were detected transcutaneously by contrast-enhanced ultrasonography alone. The number of sentinel lymph nodes detected was one or two. Despite observation of contrast enhancement of Sonazoid for at least 90 minutes, the number of sentinel lymph nodes detected did not change. The average extraction time of sentinel lymph nodes was 4.8 minutes. Indocyanine green fluorescence offered visual information during lymph node biopsy. The safety of the tracer was confirmed by absence of laryngeal edema both macro and microscopically.The combination method of indocyanine green fluorescence and contrast-enhanced ultrasonography for detecting sentinel lymph nodes during surgery for head and neck cancer seems promising, especially for pharyngeal and laryngeal cancer. Further clinical studies to confirm this are warranted. |
url |
http://europepmc.org/articles/PMC4498775?pdf=render |
work_keys_str_mv |
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