Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic Treatment
Larval oysters in hatcheries are susceptible to diseases caused by bacterial pathogens, including Vibrio spp. Previous studies have shown that daily addition of the probiotic Bacillus pumilus RI06-95 to water in rearing tanks increases larval survival when challenged with the pathogen Vibrio coralli...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-05-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01060/full |
id |
doaj-d289520d1bac46f6bfd817e4c5b206dc |
---|---|
record_format |
Article |
spelling |
doaj-d289520d1bac46f6bfd817e4c5b206dc2020-11-24T20:48:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-05-011010.3389/fmicb.2019.01060451943Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic TreatmentRebecca J. Stevick0Saebom Sohn1Tejashree H. Modak2David R. Nelson3David C. Rowley4Karin Tammi5Roxanna Smolowitz6Kathryn Markey Lundgren7Anton F. Post8Anton F. Post9Marta Gómez-Chiarri10Graduate School of Oceanography, The University of Rhode Island, Narragansett, RI, United StatesDepartment of Fisheries, Animal and Veterinary Sciences, The University of Rhode Island, Kingston, RI, United StatesDepartment of Cell and Molecular Biology, The University of Rhode Island, Kingston, RI, United StatesDepartment of Cell and Molecular Biology, The University of Rhode Island, Kingston, RI, United StatesDepartment of Biomedical and Pharmaceutical Sciences, College of Pharmacy, The University of Rhode Island, Kingston, RI, United StatesFeinstein School of Social and Natural Sciences, Roger Williams University, Bristol, RI, United StatesFeinstein School of Social and Natural Sciences, Roger Williams University, Bristol, RI, United StatesFeinstein School of Social and Natural Sciences, Roger Williams University, Bristol, RI, United StatesGraduate School of Oceanography, The University of Rhode Island, Narragansett, RI, United StatesDivision of Research, Florida Atlantic University, Boca Raton, FL, United StatesDepartment of Fisheries, Animal and Veterinary Sciences, The University of Rhode Island, Kingston, RI, United StatesLarval oysters in hatcheries are susceptible to diseases caused by bacterial pathogens, including Vibrio spp. Previous studies have shown that daily addition of the probiotic Bacillus pumilus RI06-95 to water in rearing tanks increases larval survival when challenged with the pathogen Vibrio coralliilyticus. We propose that the presence of probiotics causes shifts in bacterial community structure in rearing tanks, leading to a net decrease in the relative abundance of potential pathogens. During three trials spanning the 2012–2015 hatchery seasons, larvae, tank biofilm, and rearing water samples were collected from control and probiotic-treated tanks in an oyster hatchery over a 12-day period after spawning. Samples were analyzed by 16S rRNA sequencing of the V4 or V6 regions followed by taxonomic classification, in order to determine bacterial community structures. There were significant differences in bacterial composition over time and between sample types, but no major effect of probiotics on the structure and diversity of bacterial communities (phylum level, Bray–Curtis k = 2, 95% confidence). Probiotic treatment, however, led to a higher relative percent abundance of Oceanospirillales and Bacillus spp. in water and oyster larvae. In the water, an increase in Vibrio spp. diversity in the absence of a net increase in relative read abundance suggests a likely decrease in the abundance of specific pathogenic Vibrio spp., and therefore lower chances of a disease outbreak. Co-occurrence network analysis also suggests that probiotic treatment had a systemic effect on targeted members of the bacterial community, leading to a net decrease in potentially pathogenic species.https://www.frontiersin.org/article/10.3389/fmicb.2019.01060/fullmicrobiome16S rRNA sequencing analysisoyster hatcheryprobioticsVibrioCrassostrea virginica |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rebecca J. Stevick Saebom Sohn Tejashree H. Modak David R. Nelson David C. Rowley Karin Tammi Roxanna Smolowitz Kathryn Markey Lundgren Anton F. Post Anton F. Post Marta Gómez-Chiarri |
spellingShingle |
Rebecca J. Stevick Saebom Sohn Tejashree H. Modak David R. Nelson David C. Rowley Karin Tammi Roxanna Smolowitz Kathryn Markey Lundgren Anton F. Post Anton F. Post Marta Gómez-Chiarri Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic Treatment Frontiers in Microbiology microbiome 16S rRNA sequencing analysis oyster hatchery probiotics Vibrio Crassostrea virginica |
author_facet |
Rebecca J. Stevick Saebom Sohn Tejashree H. Modak David R. Nelson David C. Rowley Karin Tammi Roxanna Smolowitz Kathryn Markey Lundgren Anton F. Post Anton F. Post Marta Gómez-Chiarri |
author_sort |
Rebecca J. Stevick |
title |
Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic Treatment |
title_short |
Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic Treatment |
title_full |
Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic Treatment |
title_fullStr |
Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic Treatment |
title_full_unstemmed |
Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic Treatment |
title_sort |
bacterial community dynamics in an oyster hatchery in response to probiotic treatment |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-05-01 |
description |
Larval oysters in hatcheries are susceptible to diseases caused by bacterial pathogens, including Vibrio spp. Previous studies have shown that daily addition of the probiotic Bacillus pumilus RI06-95 to water in rearing tanks increases larval survival when challenged with the pathogen Vibrio coralliilyticus. We propose that the presence of probiotics causes shifts in bacterial community structure in rearing tanks, leading to a net decrease in the relative abundance of potential pathogens. During three trials spanning the 2012–2015 hatchery seasons, larvae, tank biofilm, and rearing water samples were collected from control and probiotic-treated tanks in an oyster hatchery over a 12-day period after spawning. Samples were analyzed by 16S rRNA sequencing of the V4 or V6 regions followed by taxonomic classification, in order to determine bacterial community structures. There were significant differences in bacterial composition over time and between sample types, but no major effect of probiotics on the structure and diversity of bacterial communities (phylum level, Bray–Curtis k = 2, 95% confidence). Probiotic treatment, however, led to a higher relative percent abundance of Oceanospirillales and Bacillus spp. in water and oyster larvae. In the water, an increase in Vibrio spp. diversity in the absence of a net increase in relative read abundance suggests a likely decrease in the abundance of specific pathogenic Vibrio spp., and therefore lower chances of a disease outbreak. Co-occurrence network analysis also suggests that probiotic treatment had a systemic effect on targeted members of the bacterial community, leading to a net decrease in potentially pathogenic species. |
topic |
microbiome 16S rRNA sequencing analysis oyster hatchery probiotics Vibrio Crassostrea virginica |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.01060/full |
work_keys_str_mv |
AT rebeccajstevick bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT saebomsohn bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT tejashreehmodak bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT davidrnelson bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT davidcrowley bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT karintammi bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT roxannasmolowitz bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT kathrynmarkeylundgren bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT antonfpost bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT antonfpost bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment AT martagomezchiarri bacterialcommunitydynamicsinanoysterhatcheryinresponsetoprobiotictreatment |
_version_ |
1716809093999493120 |