Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran
Introduction. Keratinophilic fungi are an important group of fungi that live in soil. The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in Shiraz. Materials and Methods. A total of 196 soil samples from 43 parks were collected. Isolation of the...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2013-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2013/619576 |
id |
doaj-d28bd07952d3462bace1419bd503d204 |
---|---|
record_format |
Article |
spelling |
doaj-d28bd07952d3462bace1419bd503d2042020-11-24T22:40:16ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/619576619576Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, IranKeyvan Pakshir0Moosa Rahimi Ghiasi1Kamiar Zomorodian2Ali Reza Gharavi3Department of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71345-45794, IranDepartment of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71345-45794, IranDepartment of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71345-45794, IranDepartment of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71345-45794, IranIntroduction. Keratinophilic fungi are an important group of fungi that live in soil. The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in Shiraz. Materials and Methods. A total of 196 soil samples from 43 parks were collected. Isolation of the fungi was performed by hair bait technique. The isolated colonies were identified by morphologic feature of macro- and microconidia and molecular method, using DNA sequence analysis. ITS region of ribosomal DNA was amplified and the PCR products were sequenced. Results. 411 isolates from 22 genera were identified. Fusarium (23.8%), Chrysosporium (13.13%), Acremonium (12.65%), Penicillium (12.39%), Microsporum gypseum (1.94%), Bionectria ochroleuca (1.21%), Bipolaris spicifera (1.21%), Scedosporium apiospermum (0.82%), Phialophora reptans (0.82%), Cephalosporium curtipes (0.49%), Scedosporium dehoogii (0.24%), Ochroconis constricta (0.24%), Nectria mauritiicola (0.49%), Chaetomium (0.49%), Scopulariopsis (0.24%), Malbranchea (0.24%), and Tritirachium (0.24%) were the most important isolates. Most of the fungi were isolated from the soils with the PH range of 7 to 8. Conclusion. Our study results showed that many keratinophilic fungi isolated from the parks soil are important for public health and children are an important group at a high risk of being exposed to these fungi.http://dx.doi.org/10.1155/2013/619576 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keyvan Pakshir Moosa Rahimi Ghiasi Kamiar Zomorodian Ali Reza Gharavi |
spellingShingle |
Keyvan Pakshir Moosa Rahimi Ghiasi Kamiar Zomorodian Ali Reza Gharavi Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran BioMed Research International |
author_facet |
Keyvan Pakshir Moosa Rahimi Ghiasi Kamiar Zomorodian Ali Reza Gharavi |
author_sort |
Keyvan Pakshir |
title |
Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran |
title_short |
Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran |
title_full |
Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran |
title_fullStr |
Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran |
title_full_unstemmed |
Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran |
title_sort |
isolation and molecular identification of keratinophilic fungi from public parks soil in shiraz, iran |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Introduction. Keratinophilic fungi are an important group of fungi that live in soil. The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in Shiraz.
Materials and Methods. A total of 196 soil samples from 43 parks were collected. Isolation of the fungi was performed by hair bait technique. The isolated colonies were identified by morphologic feature of macro- and microconidia and molecular method, using DNA sequence analysis. ITS region of ribosomal DNA was amplified and the PCR products were sequenced. Results. 411 isolates from 22 genera were identified. Fusarium (23.8%), Chrysosporium (13.13%), Acremonium (12.65%), Penicillium (12.39%), Microsporum gypseum (1.94%), Bionectria ochroleuca (1.21%), Bipolaris spicifera (1.21%), Scedosporium apiospermum (0.82%), Phialophora reptans (0.82%), Cephalosporium curtipes (0.49%), Scedosporium dehoogii (0.24%), Ochroconis constricta (0.24%), Nectria mauritiicola (0.49%), Chaetomium (0.49%), Scopulariopsis (0.24%), Malbranchea (0.24%), and Tritirachium (0.24%) were the most important isolates. Most of the fungi were isolated from the soils with the PH range of 7 to 8. Conclusion. Our study results showed that many keratinophilic fungi isolated from the parks soil are important for public health and children are an important group at a high risk of being exposed to these fungi. |
url |
http://dx.doi.org/10.1155/2013/619576 |
work_keys_str_mv |
AT keyvanpakshir isolationandmolecularidentificationofkeratinophilicfungifrompublicparkssoilinshiraziran AT moosarahimighiasi isolationandmolecularidentificationofkeratinophilicfungifrompublicparkssoilinshiraziran AT kamiarzomorodian isolationandmolecularidentificationofkeratinophilicfungifrompublicparkssoilinshiraziran AT alirezagharavi isolationandmolecularidentificationofkeratinophilicfungifrompublicparkssoilinshiraziran |
_version_ |
1725705147510685696 |