Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae
Acetic acid triggers apoptotic cell death in Saccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating-pheromone response and, especially, the cell wa...
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doaj-d299112a274b43f3b22543e8c6bc18e82020-11-24T21:20:10ZengShared Science Publishers OGMicrobial Cell2311-26382014-08-011930331410.15698/mic2014.09.164Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiaeAntónio Rego0Ana Marta Duarte1Flávio Azevedo2Maria João Sousa3Manuela Côrte-Real4Susana R. Chaves5António Rego, Ana Marta Duarte and Flávio Azevedo contributed equally to this work.António Rego, Ana Marta Duarte and Flávio Azevedo contributed equally to this work.António Rego, Ana Marta Duarte and Flávio Azevedo contributed equally to this work.Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.Acetic acid triggers apoptotic cell death in Saccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating-pheromone response and, especially, the cell wall integrity pathways were the major mediators, especially the latter, which we characterized further. Screening downstream effectors of this pathway, namely targets of the transcription factor Rlm1p, highlighted decreased cell wall remodeling as particularly important for acetic acid resistance. Modulation of cell surface dynamics therefore emerges as a powerful strategy to increase acetic acid resistance, with potential application in industrial fermentations using yeast, and in biomedicine to exploit the higher sensitivity of colorectal carcinoma cells to apoptosis induced by acetate produced by intestinal propionibacteria.http://microbialcell.com/researcharticles/cell-wall-dynamics-modulate-acetic-acid-induced-apoptotic-cell-death-of-saccharomyces-cerevisiae/yeastapoptosisacetic acidMAPKCWI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
António Rego Ana Marta Duarte Flávio Azevedo Maria João Sousa Manuela Côrte-Real Susana R. Chaves |
spellingShingle |
António Rego Ana Marta Duarte Flávio Azevedo Maria João Sousa Manuela Côrte-Real Susana R. Chaves Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae Microbial Cell yeast apoptosis acetic acid MAPK CWI |
author_facet |
António Rego Ana Marta Duarte Flávio Azevedo Maria João Sousa Manuela Côrte-Real Susana R. Chaves |
author_sort |
António Rego |
title |
Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae |
title_short |
Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae |
title_full |
Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae |
title_fullStr |
Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae |
title_full_unstemmed |
Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae |
title_sort |
cell wall dynamics modulate acetic acid-induced apoptotic cell death of saccharomyces cerevisiae |
publisher |
Shared Science Publishers OG |
series |
Microbial Cell |
issn |
2311-2638 |
publishDate |
2014-08-01 |
description |
Acetic acid triggers apoptotic cell death in Saccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating-pheromone response and, especially, the cell wall integrity pathways were the major mediators, especially the latter, which we characterized further. Screening downstream effectors of this pathway, namely targets of the transcription factor Rlm1p, highlighted decreased cell wall remodeling as particularly important for acetic acid resistance. Modulation of cell surface dynamics therefore emerges as a powerful strategy to increase acetic acid resistance, with potential application in industrial fermentations using yeast, and in biomedicine to exploit the higher sensitivity of colorectal carcinoma cells to apoptosis induced by acetate produced by intestinal propionibacteria. |
topic |
yeast apoptosis acetic acid MAPK CWI |
url |
http://microbialcell.com/researcharticles/cell-wall-dynamics-modulate-acetic-acid-induced-apoptotic-cell-death-of-saccharomyces-cerevisiae/ |
work_keys_str_mv |
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