Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8)

• Main Authors: Johanna Siehler, Anna Karolina Blöchinger, Melis Akgün, Xianming Wang, Alireza Shahryari, Arie Geerlof, Heiko Lickert, Ingo Burtscher
• Format: Article
• Language: English
• Published: Elsevier 2021-01-01
• Series: Stem Cell Research
• Online Access: http://www.sciencedirect.com/science/article/pii/S187350612030427X

The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time.