A Next-Generation Sequencing Data Analysis Pipeline for Detecting Unknown Pathogens from Mixed Clinical Samples and Revealing Their Genetic Diversity.
Forty-two cytopathic effect (CPE)-positive isolates were collected from 2008 to 2012. All isolates could not be identified for known viral pathogens by routine diagnostic assays. They were pooled into 8 groups of 5-6 isolates to reduce the sequencing cost. Next-generation sequencing (NGS) was conduc...
Main Authors: | Yu-Nong Gong, Guang-Wu Chen, Shu-Li Yang, Ching-Ju Lee, Shin-Ru Shih, Kuo-Chien Tsao |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4795770?pdf=render |
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