Effect of salvianolate therapy on peripheral blood Bcl-2, BAX and Caspase-3 expression in patients with cerebral ischemic stroke and their correlation with neural function

• Main Author: Gui-Bin Wen
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2017-05-01
• Series: Journal of Hainan Medical University
• Subjects: Cerebral ischemic stroke Salvianolate Apoptosis Neural function
• Online Access: http://www.hnykdxxb.com/PDF/201709/34.pdf

Objective: To study the peripheral blood Bcl-2, BAX and Caspase-3 expression in patients with cerebral ischemic stroke before and after salvianolate therapy and their correlation with neural function. Methods: A total of 89 patients with cerebral ischemic stroke who received salvianolate therapy in Neurology Department of Zigong Fourth People’s Hospital between May 2014 and April 2016 were studied. Before treatment as well as 2 weeks and 4 weeks after treatment, the peripheral blood apoptotic molecules Bcl-2, BAX and Caspase-3 expression as well as serum neural cytokine and oxidative stress product levels were determined respectively. Results: 2 weeks and 4 weeks after treatment, peripheral blood Bcl-2 mRNA expression as well as serum BDNF, NGF, VEGF and IGF-1 levels were significantly higher than those before treatment while BAX and Caspase-3 mRNA expression as well as serum MDA, 8-OHdG and 8-iso-PGF2α levels were significantly lower than those before treatment; 4 weeks after treatment, peripheral blood Bcl-2 mRNA expression as well as serum BDNF, NGF, VEGF and IGF-1 levels was significantly higher than those 2 weeks after treatment while BAX and Caspase-3 mRNA expression as well as serum MDA, 8-OHdG and 8-iso-PGF2α levels were significantly lower than those 2 weeks after treatment. Peripheral blood Bcl-2 mRNA expression was positively correlated with serum BDNF, NGF, VEGF and IGF-1 levels, and negatively correlated with serum MDA, 8-OHdG and 8-iso-PGF2α levels; peripheral blood BAX and Caspase-3 mRNA expression were negatively correlated with serum BDNF, NGF, VEGF and IGF-1 levels, and positively correlated with serum MDA, 8-OHdG and 8-iso-PGF2α levels. Conclusion: Salvianolate treatment of cerebral ischemic stroke can increase the antiapoptotic molecule expression and inhibit the pro-apoptotic molecule expression to improve the neural function.