Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms
High quality attenuated intracellular action potentials from large cell networks can be recorded on multi-electrode arrays by means of 3D vertical nanopillars using electrical pulses. However, most of the techniques require complex 3D nanostructures that prevent the straightforward translation into...
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2020-02-01
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doaj-d2bcfb9cdcbb40d3bdac171ccf2e57112020-11-25T02:21:35ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-02-01810.3389/fbioe.2020.00066508133Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array PlatformsGiovanni Melle0Giovanni Melle1Giulia Bruno2Giulia Bruno3Nicolò Maccaferri4Giuseppina Iachetta5Nicolò Colistra6Andrea Barbaglia7Andrea Barbaglia8Michele Dipalo9Francesco De Angelis10DIBRIS, Università degli Studi di Genova, Genova, ItalyIstituto Italiano di Tecnologia, Genova, ItalyDIBRIS, Università degli Studi di Genova, Genova, ItalyIstituto Italiano di Tecnologia, Genova, ItalyDepartment of Physics and Materials Science, University of Luxembourg, Luxembourg, LuxembourgIstituto Italiano di Tecnologia, Genova, ItalyIstituto Italiano di Tecnologia, Genova, ItalyIstituto Italiano di Tecnologia, Genova, ItalyDipartimento di Fisica, Università degli Studi di Genova, Genova, ItalyIstituto Italiano di Tecnologia, Genova, ItalyIstituto Italiano di Tecnologia, Genova, ItalyHigh quality attenuated intracellular action potentials from large cell networks can be recorded on multi-electrode arrays by means of 3D vertical nanopillars using electrical pulses. However, most of the techniques require complex 3D nanostructures that prevent the straightforward translation into marketable products and the wide adoption in the scientific community. Moreover, 3D nanostructures are often delicate objects that cannot sustain several harsh use/cleaning cycles. On the contrary, laser optoacoustic poration allows the recording of action potentials on planar nanoporous electrodes made of noble metals. However, these constraints of the electrode material and morphology may also hinder the full exploitation of this methodology. Here, we show that optoacoustic poration is also very effective for porating cells on a large family of MEA electrode configurations, including robust electrodes made of nanoporous titanium nitride or disordered fractal-like gold nanostructures. This enables the recording of high quality cardiac action potentials in combination with optoacoustic poration, providing thus attenuated intracellular recordings on various already commercial devices used by a significant part of the research and industrial communities.https://www.frontiersin.org/article/10.3389/fbioe.2020.00066/fullbioelectronicsmeta-electrodesintracellular recordingsmulti-electrode arraysoptoacoustic porationpharmacology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giovanni Melle Giovanni Melle Giulia Bruno Giulia Bruno Nicolò Maccaferri Giuseppina Iachetta Nicolò Colistra Andrea Barbaglia Andrea Barbaglia Michele Dipalo Francesco De Angelis |
spellingShingle |
Giovanni Melle Giovanni Melle Giulia Bruno Giulia Bruno Nicolò Maccaferri Giuseppina Iachetta Nicolò Colistra Andrea Barbaglia Andrea Barbaglia Michele Dipalo Francesco De Angelis Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms Frontiers in Bioengineering and Biotechnology bioelectronics meta-electrodes intracellular recordings multi-electrode arrays optoacoustic poration pharmacology |
author_facet |
Giovanni Melle Giovanni Melle Giulia Bruno Giulia Bruno Nicolò Maccaferri Giuseppina Iachetta Nicolò Colistra Andrea Barbaglia Andrea Barbaglia Michele Dipalo Francesco De Angelis |
author_sort |
Giovanni Melle |
title |
Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms |
title_short |
Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms |
title_full |
Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms |
title_fullStr |
Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms |
title_full_unstemmed |
Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms |
title_sort |
intracellular recording of human cardiac action potentials on market-available multielectrode array platforms |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2020-02-01 |
description |
High quality attenuated intracellular action potentials from large cell networks can be recorded on multi-electrode arrays by means of 3D vertical nanopillars using electrical pulses. However, most of the techniques require complex 3D nanostructures that prevent the straightforward translation into marketable products and the wide adoption in the scientific community. Moreover, 3D nanostructures are often delicate objects that cannot sustain several harsh use/cleaning cycles. On the contrary, laser optoacoustic poration allows the recording of action potentials on planar nanoporous electrodes made of noble metals. However, these constraints of the electrode material and morphology may also hinder the full exploitation of this methodology. Here, we show that optoacoustic poration is also very effective for porating cells on a large family of MEA electrode configurations, including robust electrodes made of nanoporous titanium nitride or disordered fractal-like gold nanostructures. This enables the recording of high quality cardiac action potentials in combination with optoacoustic poration, providing thus attenuated intracellular recordings on various already commercial devices used by a significant part of the research and industrial communities. |
topic |
bioelectronics meta-electrodes intracellular recordings multi-electrode arrays optoacoustic poration pharmacology |
url |
https://www.frontiersin.org/article/10.3389/fbioe.2020.00066/full |
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