A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?
Chitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmi...
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doaj-d2c0763d679945869b7bb9a82e9657262020-11-24T21:07:10ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/789591789591A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?Ming-Hui Yang0Shyng-Shiou Yuan1Ying-Fong Huang2Po-Chiao Lin3Chi-Yu Lu4Tze-Wen Chung5Yu-Chang Tyan6Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 300, TaiwanTranslational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, TaiwanDepartment of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Chemistry, National Sun Yat-sen University, Kaohsiung 804, TaiwanNational Sun Yat-sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 807, TaiwanDepartment of Biomedical Engineering, National Yang-Ming University, Taipei 112, TaiwanTranslational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, TaiwanChitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmission electron microscopy and particle size analyzer. The average size of the chitosan nanoparticle was 224.6±11.2 nm, and the average zeta potential was +14.08±0.7 mV. Moreover, using proteomic approaches to analyze the differential protein expression patterns resulted from the chitosan nanoparticle uptaken by HepG2 and CCL-13 cells identified several proteins involved in the PI3K/AKT1/mTOR pathway. Our experimental results have demonstrated that the chitosan nanoparticle may involve in the liver cancer cell metastasis and proliferation.http://dx.doi.org/10.1155/2014/789591 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming-Hui Yang Shyng-Shiou Yuan Ying-Fong Huang Po-Chiao Lin Chi-Yu Lu Tze-Wen Chung Yu-Chang Tyan |
spellingShingle |
Ming-Hui Yang Shyng-Shiou Yuan Ying-Fong Huang Po-Chiao Lin Chi-Yu Lu Tze-Wen Chung Yu-Chang Tyan A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway? BioMed Research International |
author_facet |
Ming-Hui Yang Shyng-Shiou Yuan Ying-Fong Huang Po-Chiao Lin Chi-Yu Lu Tze-Wen Chung Yu-Chang Tyan |
author_sort |
Ming-Hui Yang |
title |
A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway? |
title_short |
A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway? |
title_full |
A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway? |
title_fullStr |
A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway? |
title_full_unstemmed |
A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway? |
title_sort |
proteomic view to characterize the effect of chitosan nanoparticle to hepatic cells: is chitosan nanoparticle an enhancer of pi3k/akt1/mtor pathway? |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
Chitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmission electron microscopy and particle size analyzer. The average size of the chitosan nanoparticle was 224.6±11.2 nm, and the average zeta potential was +14.08±0.7 mV. Moreover, using proteomic approaches to analyze the differential protein expression patterns resulted from the chitosan nanoparticle uptaken by HepG2 and CCL-13 cells identified several proteins involved in the PI3K/AKT1/mTOR pathway. Our experimental results have demonstrated that the chitosan nanoparticle may involve in the liver cancer cell metastasis and proliferation. |
url |
http://dx.doi.org/10.1155/2014/789591 |
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