Dosage effect on uropathogenic <it>Escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study
<p>Abstract</p> <p>Background</p> <p>Ingestion of cranberry (<it>Vaccinium macrocarpon </it>Ait.) has traditionally been utilized for prevention of urinary tract infections. The proanthocyanidins (PACs) in cranberry, in particular the A-type linkages have be...
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doaj-d2c2e46974574276bb559b64cc943ab22020-11-25T02:58:05ZengBMCBMC Infectious Diseases1471-23342010-04-011019410.1186/1471-2334-10-94Dosage effect on uropathogenic <it>Escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind studyTenke PeterGausa LluisMatsumoto TetsuroBlanc-Potard Anne-BéatriceCombescure ChristopheBotto HenryHowell Amy BSotto AlbertLavigne Jean-Philippe<p>Abstract</p> <p>Background</p> <p>Ingestion of cranberry (<it>Vaccinium macrocarpon </it>Ait.) has traditionally been utilized for prevention of urinary tract infections. The proanthocyanidins (PACs) in cranberry, in particular the A-type linkages have been implicated as important inhibitors of primarily P-fimbriated <it>E. coli </it>adhesion to uroepithelial cells. Additional experiments were required to investigate the persistence in urine samples over a broader time period, to determine the most effective dose per day and to determine if the urinary anti-adhesion effect following cranberry is detected within volunteers of different origins.</p> <p>Methods</p> <p>Two separate bioassays (a mannose-resistant hemagglutination assay and an original new human T24 epithelial cell-line assay) have assessed the ex-vivo urinary bacterial anti-adhesion activity on urines samples collected from 32 volunteers from Japan, Hungary, Spain and France in a randomized, double-blind versus placebo study. An <it>in vivo Caenorhabditis elegans </it>model was used to evaluate the influence of cranberry regimen on the virulence of <it>E. coli </it>strain.</p> <p>Results</p> <p>The results indicated a significant bacterial anti-adhesion activity in urine samples collected from volunteers that consumed cranberry powder compared to placebo (p < 0.001). This inhibition was clearly dose-dependent, prolonged (until 24 h with 72 mg of PAC) and increasing with the amount of PAC equivalents consumed in each cranberry powder regimen. An <it>in vivo Caenorhabditis elegans </it>model showed that cranberry acted against bacterial virulence: <it>E. coli </it>strain presented a reduced ability to kill worms after a growth in urines samples of patients who took cranberry capsules. This effect is particularly important with the regimen of 72 mg of PAC.</p> <p>Conclusions</p> <p>Administration of PAC-standardized cranberry powder at dosages containing 72 mg of PAC per day may offer some protection against bacterial adhesion and virulence in the urinary tract. This effect may offer a nyctohemeral protection.</p> http://www.biomedcentral.com/1471-2334/10/94 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tenke Peter Gausa Lluis Matsumoto Tetsuro Blanc-Potard Anne-Béatrice Combescure Christophe Botto Henry Howell Amy B Sotto Albert Lavigne Jean-Philippe |
spellingShingle |
Tenke Peter Gausa Lluis Matsumoto Tetsuro Blanc-Potard Anne-Béatrice Combescure Christophe Botto Henry Howell Amy B Sotto Albert Lavigne Jean-Philippe Dosage effect on uropathogenic <it>Escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study BMC Infectious Diseases |
author_facet |
Tenke Peter Gausa Lluis Matsumoto Tetsuro Blanc-Potard Anne-Béatrice Combescure Christophe Botto Henry Howell Amy B Sotto Albert Lavigne Jean-Philippe |
author_sort |
Tenke Peter |
title |
Dosage effect on uropathogenic <it>Escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study |
title_short |
Dosage effect on uropathogenic <it>Escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study |
title_full |
Dosage effect on uropathogenic <it>Escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study |
title_fullStr |
Dosage effect on uropathogenic <it>Escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study |
title_full_unstemmed |
Dosage effect on uropathogenic <it>Escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study |
title_sort |
dosage effect on uropathogenic <it>escherichia coli </it>anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2010-04-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Ingestion of cranberry (<it>Vaccinium macrocarpon </it>Ait.) has traditionally been utilized for prevention of urinary tract infections. The proanthocyanidins (PACs) in cranberry, in particular the A-type linkages have been implicated as important inhibitors of primarily P-fimbriated <it>E. coli </it>adhesion to uroepithelial cells. Additional experiments were required to investigate the persistence in urine samples over a broader time period, to determine the most effective dose per day and to determine if the urinary anti-adhesion effect following cranberry is detected within volunteers of different origins.</p> <p>Methods</p> <p>Two separate bioassays (a mannose-resistant hemagglutination assay and an original new human T24 epithelial cell-line assay) have assessed the ex-vivo urinary bacterial anti-adhesion activity on urines samples collected from 32 volunteers from Japan, Hungary, Spain and France in a randomized, double-blind versus placebo study. An <it>in vivo Caenorhabditis elegans </it>model was used to evaluate the influence of cranberry regimen on the virulence of <it>E. coli </it>strain.</p> <p>Results</p> <p>The results indicated a significant bacterial anti-adhesion activity in urine samples collected from volunteers that consumed cranberry powder compared to placebo (p < 0.001). This inhibition was clearly dose-dependent, prolonged (until 24 h with 72 mg of PAC) and increasing with the amount of PAC equivalents consumed in each cranberry powder regimen. An <it>in vivo Caenorhabditis elegans </it>model showed that cranberry acted against bacterial virulence: <it>E. coli </it>strain presented a reduced ability to kill worms after a growth in urines samples of patients who took cranberry capsules. This effect is particularly important with the regimen of 72 mg of PAC.</p> <p>Conclusions</p> <p>Administration of PAC-standardized cranberry powder at dosages containing 72 mg of PAC per day may offer some protection against bacterial adhesion and virulence in the urinary tract. This effect may offer a nyctohemeral protection.</p> |
url |
http://www.biomedcentral.com/1471-2334/10/94 |
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