Coating-Dependent Effects of Silver Nanoparticles on Tobacco Seed Germination and Early Growth

• Main Authors: Renata Biba, Dajana Matić, Daniel Mark Lyons, Petra Peharec Štefanić, Petra Cvjetko, Mirta Tkalec, Dubravko Pavoković, Ilse Letofsky-Papst, Biljana Balen
• Format: Article
• Language: English
• Published: MDPI AG 2020-05-01
• Series: International Journal of Molecular Sciences
• Subjects: silver nanoparticles; AgNP stability; <i>Nicotiana tabacum</i> L.; germination; root length; fresh and dry weight
• Online Access: https://www.mdpi.com/1422-0067/21/10/3441

Silver nanoparticles (AgNPs) are used in a wide range of consumer products because of their excellent antimicrobial properties. AgNPs released into the environment are prone to transformations such as aggregation, oxidation, or dissolution so they are often stabilised by coatings that affect their physico-chemical properties and change their effect on living organisms. In this study we investigated the stability of polyvinylpyrrolidone (PVP) and cetyltrimethylammonium bromide (CTAB) coated AgNPs in an exposure medium, as well as their effect on tobacco germination and early growth. AgNP-CTAB was found to be more stable in the solid Murashige and Skoog (MS) medium compared to AgNP-PVP. The uptake and accumulation of silver in seedlings was equally efficient after exposure to both types of AgNPs. However, AgNP-PVP induced only mild toxicity on seedlings growth, while AgNP-CTAB caused severe negative effects on all parameters, even compared to AgNO₃. Moreover, CTAB coating itself exerted negative effects on growth. Cysteine addition generally alleviated AgNP-PVP-induced negative effects, while it failed to improve germination and growth parameters after exposure to AgNP-CTAB. These results suggest that the toxic effects of AgNP-PVP are mainly a consequence of release of Ag⁺ ions, while phytotoxicity of AgNP-CTAB can rather be ascribed to surface coating itself.