A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells
We show that a cage-shaped F-actin network is essential for maintaining a tight spatial organization of Cav1.3 Ca2+ channels at the synaptic ribbons of auditory inner hair cells. This F-actin network is also found to provide mechanosensitivity to the Cav1.3 channels when varying intracellular hydros...
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doaj-d2dc8032c0814d8796d5539d1a3a64aa2021-05-05T00:06:30ZengeLife Sciences Publications LtdeLife2050-084X2015-11-01410.7554/eLife.10988A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cellsPhilippe FY Vincent0Yohan Bouleau1Christine Petit2Didier Dulon3Bordeaux Neurocampus, Equipe Neurophysiologie de la Synapse Auditive, Université de Bordeaux, Bordeaux, FranceBordeaux Neurocampus, Equipe Neurophysiologie de la Synapse Auditive, Université de Bordeaux, Bordeaux, FranceUnité de Génétique et Physiologie de l’Audition, Institut Pasteur, Paris, France; UMRS 1120, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France; Sorbonne Universités, UPMC Université Paris, Paris, France; Syndrome de Usher et Autres Atteintes Rétino-Cochléaires, Institut de la Vision, Paris, France; Collège de France, Paris, FranceBordeaux Neurocampus, Equipe Neurophysiologie de la Synapse Auditive, Université de Bordeaux, Bordeaux, France; UMRS 1120, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, FranceWe show that a cage-shaped F-actin network is essential for maintaining a tight spatial organization of Cav1.3 Ca2+ channels at the synaptic ribbons of auditory inner hair cells. This F-actin network is also found to provide mechanosensitivity to the Cav1.3 channels when varying intracellular hydrostatic pressure. Furthermore, this F-actin mesh network attached to the synaptic ribbons directly influences the efficiency of otoferlin-dependent exocytosis and its sensitivity to intracellular hydrostatic pressure, independently of its action on the Cav1.3 channels. We propose a new mechanistic model for vesicle exocytosis in auditory hair cells where the rate of vesicle recruitment to the ribbons is directly controlled by a synaptic F-actin network and changes in intracellular hydrostatic pressure.https://elifesciences.org/articles/10988exocytosishair cellactin networkosmotic pressure |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Philippe FY Vincent Yohan Bouleau Christine Petit Didier Dulon |
spellingShingle |
Philippe FY Vincent Yohan Bouleau Christine Petit Didier Dulon A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells eLife exocytosis hair cell actin network osmotic pressure |
author_facet |
Philippe FY Vincent Yohan Bouleau Christine Petit Didier Dulon |
author_sort |
Philippe FY Vincent |
title |
A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells |
title_short |
A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells |
title_full |
A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells |
title_fullStr |
A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells |
title_full_unstemmed |
A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells |
title_sort |
synaptic f-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2015-11-01 |
description |
We show that a cage-shaped F-actin network is essential for maintaining a tight spatial organization of Cav1.3 Ca2+ channels at the synaptic ribbons of auditory inner hair cells. This F-actin network is also found to provide mechanosensitivity to the Cav1.3 channels when varying intracellular hydrostatic pressure. Furthermore, this F-actin mesh network attached to the synaptic ribbons directly influences the efficiency of otoferlin-dependent exocytosis and its sensitivity to intracellular hydrostatic pressure, independently of its action on the Cav1.3 channels. We propose a new mechanistic model for vesicle exocytosis in auditory hair cells where the rate of vesicle recruitment to the ribbons is directly controlled by a synaptic F-actin network and changes in intracellular hydrostatic pressure. |
topic |
exocytosis hair cell actin network osmotic pressure |
url |
https://elifesciences.org/articles/10988 |
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