The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability
The whole plant of Anisomeles ovata has been widely used in Taiwan for treating inflammation-related skin and liver diseases, however, the detailed pharmacology mechanisms have yet to be elucidated. In the present study, one of the major components, 5,6,4′-trihydroxy-7,3′-dimethoxyflavone (5-TDMF),...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2016-01-01
|
Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/21/2/136 |
id |
doaj-d2e21351650640169d42ec66d73aea23 |
---|---|
record_format |
Article |
spelling |
doaj-d2e21351650640169d42ec66d73aea232020-11-25T00:19:22ZengMDPI AGMolecules1420-30492016-01-0121213610.3390/molecules21020136molecules21020136The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant AbilityShih-Hao Wang0Chia-Hua Liang1Fong-Pin Liang2Hsiou-Yu Ding3Shiuan-Pey Lin4Guan-Jhong Huang5Wen-Chuan Lin6Shin-Hun Juang7School of Pharmacy, China Medical University, Taichung 404, TaiwanDepartment of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, TaiwanDepartment of Pharmacy, Tajen University, Pingtung 907, TaiwanInstitute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, TaiwanSchool of Pharmacy, China Medical University, Taichung 404, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, TaiwanSchool of Pharmacy, China Medical University, Taichung 404, TaiwanDepartment of Pharmacy, Tajen University, Pingtung 907, TaiwanThe whole plant of Anisomeles ovata has been widely used in Taiwan for treating inflammation-related skin and liver diseases, however, the detailed pharmacology mechanisms have yet to be elucidated. In the present study, one of the major components, 5,6,4′-trihydroxy-7,3′-dimethoxyflavone (5-TDMF), was purified from a methanol extract of Anisomeles ovata. A pharmacological study of this compound suggests that 5-TDMF possesses potent free radical scavenging activity both in vitro and ex vivo. Furthermore, 5-TDMF reduces nitric oxide and pro-inflammatory cytokine production in LPC-treated RAW 264.7 cells through the attenuation of nitric oxide synthase and cyclooxygenase-2. Additional experiments suggest that of 5-TDMF interferes with nuclear factor-κB translocation and mitogen-activated protein kinase pathways. These results identify 5-TDMF as an anti-oxidant and anti-inflammatory compound, explain the pharmacologic function of Anisomeles ovata and suggest its great potential as a new anti-inflammatory remedy.http://www.mdpi.com/1420-3049/21/2/136Anisomeles ovata5,6,4′-trihydroxy-7,3′-dimethoxyflavoneanti-inflammatoryanti-oxidantreactive oxygen species |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shih-Hao Wang Chia-Hua Liang Fong-Pin Liang Hsiou-Yu Ding Shiuan-Pey Lin Guan-Jhong Huang Wen-Chuan Lin Shin-Hun Juang |
spellingShingle |
Shih-Hao Wang Chia-Hua Liang Fong-Pin Liang Hsiou-Yu Ding Shiuan-Pey Lin Guan-Jhong Huang Wen-Chuan Lin Shin-Hun Juang The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability Molecules Anisomeles ovata 5,6,4′-trihydroxy-7,3′-dimethoxyflavone anti-inflammatory anti-oxidant reactive oxygen species |
author_facet |
Shih-Hao Wang Chia-Hua Liang Fong-Pin Liang Hsiou-Yu Ding Shiuan-Pey Lin Guan-Jhong Huang Wen-Chuan Lin Shin-Hun Juang |
author_sort |
Shih-Hao Wang |
title |
The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability |
title_short |
The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability |
title_full |
The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability |
title_fullStr |
The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability |
title_full_unstemmed |
The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability |
title_sort |
inhibitory mechanisms study of 5,6,4′-trihydroxy-7,3′-dimethoxyflavone against the lps-induced macrophage inflammatory responses through the antioxidant ability |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2016-01-01 |
description |
The whole plant of Anisomeles ovata has been widely used in Taiwan for treating inflammation-related skin and liver diseases, however, the detailed pharmacology mechanisms have yet to be elucidated. In the present study, one of the major components, 5,6,4′-trihydroxy-7,3′-dimethoxyflavone (5-TDMF), was purified from a methanol extract of Anisomeles ovata. A pharmacological study of this compound suggests that 5-TDMF possesses potent free radical scavenging activity both in vitro and ex vivo. Furthermore, 5-TDMF reduces nitric oxide and pro-inflammatory cytokine production in LPC-treated RAW 264.7 cells through the attenuation of nitric oxide synthase and cyclooxygenase-2. Additional experiments suggest that of 5-TDMF interferes with nuclear factor-κB translocation and mitogen-activated protein kinase pathways. These results identify 5-TDMF as an anti-oxidant and anti-inflammatory compound, explain the pharmacologic function of Anisomeles ovata and suggest its great potential as a new anti-inflammatory remedy. |
topic |
Anisomeles ovata 5,6,4′-trihydroxy-7,3′-dimethoxyflavone anti-inflammatory anti-oxidant reactive oxygen species |
url |
http://www.mdpi.com/1420-3049/21/2/136 |
work_keys_str_mv |
AT shihhaowang theinhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT chiahualiang theinhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT fongpinliang theinhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT hsiouyuding theinhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT shiuanpeylin theinhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT guanjhonghuang theinhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT wenchuanlin theinhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT shinhunjuang theinhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT shihhaowang inhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT chiahualiang inhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT fongpinliang inhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT hsiouyuding inhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT shiuanpeylin inhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT guanjhonghuang inhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT wenchuanlin inhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability AT shinhunjuang inhibitorymechanismsstudyof564trihydroxy73dimethoxyflavoneagainstthelpsinducedmacrophageinflammatoryresponsesthroughtheantioxidantability |
_version_ |
1725371850988453888 |