Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae)
CRY-DASH, a new cryptochrome blue light receptor, can repair damaged DNA and regulate secondary metabolism and development of fungus. However, its role in regulation during the growth of <i>Saccharina japonica</i> is still unclear. After cloning the full-length of <i>CRY-DASH</i...
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doaj-d2e7043fd55849858f651e9b2db7e2af2020-11-25T02:13:31ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121130910.3390/ijms21010309ijms21010309Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae)Xiaoqi Yang0Lu Li1Xiuliang Wang2Jianting Yao3Delin Duan4Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCRY-DASH, a new cryptochrome blue light receptor, can repair damaged DNA and regulate secondary metabolism and development of fungus. However, its role in regulation during the growth of <i>Saccharina japonica</i> is still unclear. After cloning the full-length of <i>CRY-DASH</i> from <i>S. japonica</i> (<i>sjCRY-DASH</i>), we deduced that its open reading frame was 1779 bp long and encoded 592 amino acids. <i>sjCRY-DASH</i> transcription was rapidly upregulated within 30 min in response to blue light and exhibited 24 h periodicity with different photoperiods. Moreover, <i>sjCRY-DASH</i> maintained the same periodicity in suitable growth temperature, suggesting a close relationship between this periodicity and circadian rhythm regulation. <i>Novel-m3234-5p</i>, which was targeted to <i>sjCRY-DASH</i>, decreased with increasing <i>sjCRY-DASH</i> transcription, acting as a negative modulator of <i>sjCRY-DASH</i>. Six long non-coding RNAs classified as long intergenic non-coding RNAs (lincRNAs) exhibited co-expression with <i>sjCRY-DASH</i>. A miRNA <i>sjCRY DASH</i> lincRNA network was consequently identified. By predicting the endogenous competing mRNAs of <i>novel</i>-<i>m3234</i>-<i>5p</i>, we found that <i>sjCRY-DASH</i> indirectly participated in the regulation of DNA damage repair, protein synthesis and processing, and actin transport. In conclusion, our results revealed that non-coding RNAs participate in the regulation of <i>sjCRY-DASH,</i> which played vital roles in the growth and early development of <i>S. japonica</i>.https://www.mdpi.com/1422-0067/21/1/309blue lightcircadian rhythmcry-dashnon-coding rnagrowth<i>saccharina japonica</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoqi Yang Lu Li Xiuliang Wang Jianting Yao Delin Duan |
spellingShingle |
Xiaoqi Yang Lu Li Xiuliang Wang Jianting Yao Delin Duan Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae) International Journal of Molecular Sciences blue light circadian rhythm cry-dash non-coding rna growth <i>saccharina japonica</i> |
author_facet |
Xiaoqi Yang Lu Li Xiuliang Wang Jianting Yao Delin Duan |
author_sort |
Xiaoqi Yang |
title |
Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae) |
title_short |
Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae) |
title_full |
Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae) |
title_fullStr |
Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae) |
title_full_unstemmed |
Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae) |
title_sort |
non-coding rnas participate in the regulation of <i>cry-dash</i> in the growth and early development of <i>saccharina japonica</i> (laminariales, phaeophyceae) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-01-01 |
description |
CRY-DASH, a new cryptochrome blue light receptor, can repair damaged DNA and regulate secondary metabolism and development of fungus. However, its role in regulation during the growth of <i>Saccharina japonica</i> is still unclear. After cloning the full-length of <i>CRY-DASH</i> from <i>S. japonica</i> (<i>sjCRY-DASH</i>), we deduced that its open reading frame was 1779 bp long and encoded 592 amino acids. <i>sjCRY-DASH</i> transcription was rapidly upregulated within 30 min in response to blue light and exhibited 24 h periodicity with different photoperiods. Moreover, <i>sjCRY-DASH</i> maintained the same periodicity in suitable growth temperature, suggesting a close relationship between this periodicity and circadian rhythm regulation. <i>Novel-m3234-5p</i>, which was targeted to <i>sjCRY-DASH</i>, decreased with increasing <i>sjCRY-DASH</i> transcription, acting as a negative modulator of <i>sjCRY-DASH</i>. Six long non-coding RNAs classified as long intergenic non-coding RNAs (lincRNAs) exhibited co-expression with <i>sjCRY-DASH</i>. A miRNA <i>sjCRY DASH</i> lincRNA network was consequently identified. By predicting the endogenous competing mRNAs of <i>novel</i>-<i>m3234</i>-<i>5p</i>, we found that <i>sjCRY-DASH</i> indirectly participated in the regulation of DNA damage repair, protein synthesis and processing, and actin transport. In conclusion, our results revealed that non-coding RNAs participate in the regulation of <i>sjCRY-DASH,</i> which played vital roles in the growth and early development of <i>S. japonica</i>. |
topic |
blue light circadian rhythm cry-dash non-coding rna growth <i>saccharina japonica</i> |
url |
https://www.mdpi.com/1422-0067/21/1/309 |
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