Non-Coding RNAs Participate in the Regulation of <i>CRY-DASH</i> in the Growth and Early Development of <i>Saccharina japonica</i> (Laminariales, Phaeophyceae)

• Main Authors: Xiaoqi Yang, Lu Li, Xiuliang Wang, Jianting Yao, Delin Duan
• Format: Article
• Language: English
• Published: MDPI AG 2020-01-01
• Series: International Journal of Molecular Sciences
• Subjects: blue light; circadian rhythm; cry-dash; non-coding rna; growth; <i>saccharina japonica</i>
• Online Access: https://www.mdpi.com/1422-0067/21/1/309

CRY-DASH, a new cryptochrome blue light receptor, can repair damaged DNA and regulate secondary metabolism and development of fungus. However, its role in regulation during the growth of <i>Saccharina japonica</i> is still unclear. After cloning the full-length of <i>CRY-DASH</i> from <i>S. japonica</i> (<i>sjCRY-DASH</i>), we deduced that its open reading frame was 1779 bp long and encoded 592 amino acids. <i>sjCRY-DASH</i> transcription was rapidly upregulated within 30 min in response to blue light and exhibited 24 h periodicity with different photoperiods. Moreover, <i>sjCRY-DASH</i> maintained the same periodicity in suitable growth temperature, suggesting a close relationship between this periodicity and circadian rhythm regulation. <i>Novel-m3234-5p</i>, which was targeted to <i>sjCRY-DASH</i>, decreased with increasing <i>sjCRY-DASH</i> transcription, acting as a negative modulator of <i>sjCRY-DASH</i>. Six long non-coding RNAs classified as long intergenic non-coding RNAs (lincRNAs) exhibited co-expression with <i>sjCRY-DASH</i>. A miRNA <i>sjCRY DASH</i> lincRNA network was consequently identified. By predicting the endogenous competing mRNAs of <i>novel</i>-<i>m3234</i>-<i>5p</i>, we found that <i>sjCRY-DASH</i> indirectly participated in the regulation of DNA damage repair, protein synthesis and processing, and actin transport. In conclusion, our results revealed that non-coding RNAs participate in the regulation of <i>sjCRY-DASH,</i> which played vital roles in the growth and early development of <i>S. japonica</i>.