Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts
Abstract Background Thermotolerance is a highly desirable trait of microbial cell factories and has been the focus of extensive research. Yeast usually tolerate only a narrow temperature range and just two species, Kluyveromyces marxianus and Ogataea polymorpha have been described to grow at reasona...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2019-05-01
|
Series: | BMC Microbiology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12866-019-1453-3 |
id |
doaj-d30c1a3671e0466b94edf0190525ee6d |
---|---|
record_format |
Article |
spelling |
doaj-d30c1a3671e0466b94edf0190525ee6d2020-11-25T03:27:20ZengBMCBMC Microbiology1471-21802019-05-0119111110.1186/s12866-019-1453-3Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeastsMathias Lehnen0Birgitta E. Ebert1Lars M. Blank2iAMB – Institute of Applied Microbiology, ABBt – Aachen Biology and Biotechnology, RWTH Aachen UniversityiAMB – Institute of Applied Microbiology, ABBt – Aachen Biology and Biotechnology, RWTH Aachen UniversityiAMB – Institute of Applied Microbiology, ABBt – Aachen Biology and Biotechnology, RWTH Aachen UniversityAbstract Background Thermotolerance is a highly desirable trait of microbial cell factories and has been the focus of extensive research. Yeast usually tolerate only a narrow temperature range and just two species, Kluyveromyces marxianus and Ogataea polymorpha have been described to grow at reasonable rates above 40 °C. However, the complex mechanisms of thermotolerance in yeast impede its full comprehension and the rare physiological data at elevated temperatures has so far not been matched with corresponding metabolic analyses. Results To elaborate on the metabolic network response to increased fermentation temperatures of up to 49 °C, comprehensive physiological datasets of several Kluyveromyces and Ogataea strains were generated and used for 13C-metabolic flux analyses. While the maximum growth temperature was very similar in all investigated strains, the metabolic network response to elevated temperatures was not conserved among the different species. In fact, metabolic flux distributions were remarkably irresponsive to increasing temperatures in O. polymorpha, while the K. marxianus strains exhibited extensive flux rerouting at elevated temperatures. Conclusions While a clear mechanism of thermotolerance is not deducible from the fluxome level alone, the generated data can be valued as a knowledge repository for using temperature to modulate the metabolic activity towards engineering goals.http://link.springer.com/article/10.1186/s12866-019-1453-3ThermotoleranceQuantitative physiology13C-metabolic flux analysisKluyveromyces marxianusOgataea (Hansenula) polymorphaMetabolism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mathias Lehnen Birgitta E. Ebert Lars M. Blank |
spellingShingle |
Mathias Lehnen Birgitta E. Ebert Lars M. Blank Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts BMC Microbiology Thermotolerance Quantitative physiology 13C-metabolic flux analysis Kluyveromyces marxianus Ogataea (Hansenula) polymorpha Metabolism |
author_facet |
Mathias Lehnen Birgitta E. Ebert Lars M. Blank |
author_sort |
Mathias Lehnen |
title |
Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts |
title_short |
Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts |
title_full |
Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts |
title_fullStr |
Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts |
title_full_unstemmed |
Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts |
title_sort |
elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2019-05-01 |
description |
Abstract Background Thermotolerance is a highly desirable trait of microbial cell factories and has been the focus of extensive research. Yeast usually tolerate only a narrow temperature range and just two species, Kluyveromyces marxianus and Ogataea polymorpha have been described to grow at reasonable rates above 40 °C. However, the complex mechanisms of thermotolerance in yeast impede its full comprehension and the rare physiological data at elevated temperatures has so far not been matched with corresponding metabolic analyses. Results To elaborate on the metabolic network response to increased fermentation temperatures of up to 49 °C, comprehensive physiological datasets of several Kluyveromyces and Ogataea strains were generated and used for 13C-metabolic flux analyses. While the maximum growth temperature was very similar in all investigated strains, the metabolic network response to elevated temperatures was not conserved among the different species. In fact, metabolic flux distributions were remarkably irresponsive to increasing temperatures in O. polymorpha, while the K. marxianus strains exhibited extensive flux rerouting at elevated temperatures. Conclusions While a clear mechanism of thermotolerance is not deducible from the fluxome level alone, the generated data can be valued as a knowledge repository for using temperature to modulate the metabolic activity towards engineering goals. |
topic |
Thermotolerance Quantitative physiology 13C-metabolic flux analysis Kluyveromyces marxianus Ogataea (Hansenula) polymorpha Metabolism |
url |
http://link.springer.com/article/10.1186/s12866-019-1453-3 |
work_keys_str_mv |
AT mathiaslehnen elevatedtemperaturesdonottriggeraconservedmetabolicnetworkresponseamongthermotolerantyeasts AT birgittaeebert elevatedtemperaturesdonottriggeraconservedmetabolicnetworkresponseamongthermotolerantyeasts AT larsmblank elevatedtemperaturesdonottriggeraconservedmetabolicnetworkresponseamongthermotolerantyeasts |
_version_ |
1724588186922909696 |