Anti-α-Glucosidase Activity by a Protease from <i>Bacillus licheniformis</i>
Anti-α-glucosidase (AAG) compounds have received great attention due to their potential use in treating diabetes. In this study, <i>Bacillus licheniformis</i> TKU004, an isolated bacterial strain from Taiwanese soil, produced AAG activity in the culture supernatant when squid pe...
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doaj-d30c1be57b0c48eba49919f818d628a42020-11-25T00:31:05ZengMDPI AGMolecules1420-30492019-02-0124469110.3390/molecules24040691molecules24040691Anti-α-Glucosidase Activity by a Protease from <i>Bacillus licheniformis</i>Chien Thang Doan0Thi Ngoc Tran1Minh Trung Nguyen2Van Bon Nguyen3Anh Dzung Nguyen4San-Lang Wang5Department of Chemistry, Tamkang University, New Taipei City 25137, TaiwanDepartment of Chemistry, Tamkang University, New Taipei City 25137, TaiwanDepartment of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, VietnamDepartment of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, VietnamInstitute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, VietnamDepartment of Chemistry, Tamkang University, New Taipei City 25137, TaiwanAnti-α-glucosidase (AAG) compounds have received great attention due to their potential use in treating diabetes. In this study, <i>Bacillus licheniformis</i> TKU004, an isolated bacterial strain from Taiwanese soil, produced AAG activity in the culture supernatant when squid pens were used as the sole carbon/nitrogen (C/N) source. The protein TKU004P, which was isolated from <i>B. licheniformis</i> TKU004, showed stronger AAG activity than acarbose, a commercial anti-diabetic drug (IC<sub>50</sub> = 0.1 mg/mL and 2.02 mg/mL, respectively). The molecular weight of TKU004P, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was 29 kDa. High-performance liquid chromatography (HPLC) analysis showed that TKU004P may be a protease that demonstrates AAG activity by degrading yeast α-glucosidase. Among the four chitinous sources of C/N, TKU004P produced the highest AAG activity in the culture supernatant when shrimp head powder was used as the sole source (470.66 U/mL). For comparison, 16 proteases, were investigated for AAG activity but TKU004P produced the highest levels. Overall, the findings suggest that TKU004P could have applications in the biochemical and medicinal fields thanks to its ability to control the activity of α-glucosidase.https://www.mdpi.com/1420-3049/24/4/691anti-α-glucosidaseproteasediabetesmicrobial conversion<i>Bacillus licheniformis</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chien Thang Doan Thi Ngoc Tran Minh Trung Nguyen Van Bon Nguyen Anh Dzung Nguyen San-Lang Wang |
spellingShingle |
Chien Thang Doan Thi Ngoc Tran Minh Trung Nguyen Van Bon Nguyen Anh Dzung Nguyen San-Lang Wang Anti-α-Glucosidase Activity by a Protease from <i>Bacillus licheniformis</i> Molecules anti-α-glucosidase protease diabetes microbial conversion <i>Bacillus licheniformis</i> |
author_facet |
Chien Thang Doan Thi Ngoc Tran Minh Trung Nguyen Van Bon Nguyen Anh Dzung Nguyen San-Lang Wang |
author_sort |
Chien Thang Doan |
title |
Anti-α-Glucosidase Activity by a Protease from <i>Bacillus licheniformis</i> |
title_short |
Anti-α-Glucosidase Activity by a Protease from <i>Bacillus licheniformis</i> |
title_full |
Anti-α-Glucosidase Activity by a Protease from <i>Bacillus licheniformis</i> |
title_fullStr |
Anti-α-Glucosidase Activity by a Protease from <i>Bacillus licheniformis</i> |
title_full_unstemmed |
Anti-α-Glucosidase Activity by a Protease from <i>Bacillus licheniformis</i> |
title_sort |
anti-α-glucosidase activity by a protease from <i>bacillus licheniformis</i> |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-02-01 |
description |
Anti-α-glucosidase (AAG) compounds have received great attention due to their potential use in treating diabetes. In this study, <i>Bacillus licheniformis</i> TKU004, an isolated bacterial strain from Taiwanese soil, produced AAG activity in the culture supernatant when squid pens were used as the sole carbon/nitrogen (C/N) source. The protein TKU004P, which was isolated from <i>B. licheniformis</i> TKU004, showed stronger AAG activity than acarbose, a commercial anti-diabetic drug (IC<sub>50</sub> = 0.1 mg/mL and 2.02 mg/mL, respectively). The molecular weight of TKU004P, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was 29 kDa. High-performance liquid chromatography (HPLC) analysis showed that TKU004P may be a protease that demonstrates AAG activity by degrading yeast α-glucosidase. Among the four chitinous sources of C/N, TKU004P produced the highest AAG activity in the culture supernatant when shrimp head powder was used as the sole source (470.66 U/mL). For comparison, 16 proteases, were investigated for AAG activity but TKU004P produced the highest levels. Overall, the findings suggest that TKU004P could have applications in the biochemical and medicinal fields thanks to its ability to control the activity of α-glucosidase. |
topic |
anti-α-glucosidase protease diabetes microbial conversion <i>Bacillus licheniformis</i> |
url |
https://www.mdpi.com/1420-3049/24/4/691 |
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