Ekstrak Etanol Propolis Isolat Menurunkan Derajat Inflamasi dan Kadar Malondialdehid pada Serum Tikus Model Sepsis

• Main Authors: Diding Heri Prasetyo, Endang Listyaningsih Suparyanti, A. Guntur H.
• Format: Article
• Language: English
• Published: Universitas Padjajaran 2013-09-01
• Series: Majalah Kedokteran Bandung
• Subjects: Inflammation; malondialdehyde; propolis; sepsis
• Online Access: http://journal.fk.unpad.ac.id/index.php/mkb/article/view/146
• ISSN: 0126-074X; 2338-6223

The role of bee propolis as a adjuvant therapy in the management of sepsis was evaluated. The aim of the study was to investigate the protective effect of propolis against cecal inoculum induced sepsis, the level of intestinal inflammation, and serum malondialdehyde (MDA) concentrations. The study was conducted at School of Medicine, Sebelas Maret University, Surakarta, in April to September 2011. Forty male Rattus norvegicus L rats were divided into five groups: control, sepsis, sepsis+antibiotic, sepsis+propolis and propolis+antibiotic+sepsis groups. On the eighth day, all experimental animals were sacrificed. Serum concentrations of MDA were evaluated. In addition, the histopathological changes in intestinal were assessed. Kruskall-Wallis test with Mann-Whitney analysis were used to determine significant differences. Results were expressed as mean±standard error of the mean, and value of p<0.05 was considered statistically significant. Malondialdehyde serum means levels were control group 0.27±0.07 μmol/L, sepsis 0.39±0.05 μmol/L, sepsis+antibiotic 0.15±0.03 μmol/L, sepsis+ propolis 0.09±0.05 μmol/L, and sepsis+antibiotic+propolis 0.21±0.11 μmol/L respectively. The levels of intestinal inflammation were control groups 0 to 3, sepsis 3 to 4, sepsis+antibiotic 1 to 3, sepsis+propolis 1 to 3, and sepsis+antibiotic+propolis 0 to 2, respectively. In conclusions, ethanol extract of propolis reduces the levels of intestinal inflammation and serum MDA in sepsis animal models.