Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts
This study shows the seasonal effect on the antioxidant, antiproliferative, and antimicrobial activities of L. glaucescens Kunth (LG) leaves extracts. Their antioxidant activity was evaluated through the DPPH, FRAP, and ORAC assays. Their phenolic content (PC) was determined by means of the Folin-Ci...
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doaj-d32974f67cca4ed29125af277b4496922020-11-25T00:21:48ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882018-01-01201810.1155/2018/27384892738489Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth ExtractsJulio César López-Romero0Humberto González-Ríos1Aida Peña-Ramos2Carlos Velazquez3Moises Navarro4Ramón Robles-Zepeda5Evelin Martínez-Benavidez6Inocencio Higuera-Ciapara7Claudia Virués8José Luis Olivares9Zaira Domínguez10Javier Hernández11Centro de Investigación en Alimentación y Desarrollo (CIAD), 83000 Hermosillo, SON, MexicoCentro de Investigación en Alimentación y Desarrollo (CIAD), 83000 Hermosillo, SON, MexicoCentro de Investigación en Alimentación y Desarrollo (CIAD), 83000 Hermosillo, SON, MexicoDepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Rosales y Luis Encinas, 83000 Hermosillo, SON, MexicoDepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Rosales y Luis Encinas, 83000 Hermosillo, SON, MexicoDepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Rosales y Luis Encinas, 83000 Hermosillo, SON, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., CONSORCIO-ADESUR, Av. Normalistas 800, Colinas de la Normal, 44270 Guadalajara, JAL, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., CONSORCIO-ADESUR, Av. Normalistas 800, Colinas de la Normal, 44270 Guadalajara, JAL, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Clúster Científico y Tecnológico Biomimic®, Carretera Antigua a Coatepec No. 351, Colonia El Haya, 91070 Xalapa, VER, MexicoRed de Estudios Moleculares Avanzados, Clúster Biomimic®, Instituto de Ecología, A. C., Carretera Antigua a Coatepec No. 351, Colonia El Haya, 91070 Xalapa, VER, MexicoUnidad de Servicios de Apoyo en Resolución Analítica, Universidad Veracruzana, 575 Xalapa, VER, MexicoUnidad de Servicios de Apoyo en Resolución Analítica, Universidad Veracruzana, 575 Xalapa, VER, MexicoThis study shows the seasonal effect on the antioxidant, antiproliferative, and antimicrobial activities of L. glaucescens Kunth (LG) leaves extracts. Their antioxidant activity was evaluated through the DPPH, FRAP, and ORAC assays. Their phenolic content (PC) was determined by means of the Folin-Ciocalteu method, and the main phenolic compounds were identified through a HPLC-DAD analysis. Antiproliferative activity was determined by MTT assay against HeLa, LS 180, M12.C3.F6, and ARPE cell lines. Antimicrobial potential was evaluated against Staphylococcus aureus and Escherichia coli using a microdilution method. All the LG extracts presented high antioxidant activity and PC, with quercitrin and epicatechin being the most abundant. Antioxidant activity and PC were affected by the season; particularly autumn (ALGE) and summer (SULGE) extracts exhibited the highest values (p<0.05). All extracts presented moderate antiproliferative activity against the cell lines evaluated, HeLa being the most susceptible of them. However, ALGE and SULGE were the most active too. About antimicrobial activity, SULGE (MIC90<800 μg/mL; MIC50 < 400 μg/mL), and SLGE (MIC50 < 1000 μg/mL) showed a moderate inhibitory effect against S. aureus. These findings provide new information about the seasonal effect on the PC and biological properties of LG extracts. Clearly, antioxidant activity was the most important with respect to the other two.http://dx.doi.org/10.1155/2018/2738489 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julio César López-Romero Humberto González-Ríos Aida Peña-Ramos Carlos Velazquez Moises Navarro Ramón Robles-Zepeda Evelin Martínez-Benavidez Inocencio Higuera-Ciapara Claudia Virués José Luis Olivares Zaira Domínguez Javier Hernández |
spellingShingle |
Julio César López-Romero Humberto González-Ríos Aida Peña-Ramos Carlos Velazquez Moises Navarro Ramón Robles-Zepeda Evelin Martínez-Benavidez Inocencio Higuera-Ciapara Claudia Virués José Luis Olivares Zaira Domínguez Javier Hernández Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts Evidence-Based Complementary and Alternative Medicine |
author_facet |
Julio César López-Romero Humberto González-Ríos Aida Peña-Ramos Carlos Velazquez Moises Navarro Ramón Robles-Zepeda Evelin Martínez-Benavidez Inocencio Higuera-Ciapara Claudia Virués José Luis Olivares Zaira Domínguez Javier Hernández |
author_sort |
Julio César López-Romero |
title |
Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts |
title_short |
Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts |
title_full |
Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts |
title_fullStr |
Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts |
title_full_unstemmed |
Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts |
title_sort |
seasonal effect on the biological activities of litsea glaucescens kunth extracts |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2018-01-01 |
description |
This study shows the seasonal effect on the antioxidant, antiproliferative, and antimicrobial activities of L. glaucescens Kunth (LG) leaves extracts. Their antioxidant activity was evaluated through the DPPH, FRAP, and ORAC assays. Their phenolic content (PC) was determined by means of the Folin-Ciocalteu method, and the main phenolic compounds were identified through a HPLC-DAD analysis. Antiproliferative activity was determined by MTT assay against HeLa, LS 180, M12.C3.F6, and ARPE cell lines. Antimicrobial potential was evaluated against Staphylococcus aureus and Escherichia coli using a microdilution method. All the LG extracts presented high antioxidant activity and PC, with quercitrin and epicatechin being the most abundant. Antioxidant activity and PC were affected by the season; particularly autumn (ALGE) and summer (SULGE) extracts exhibited the highest values (p<0.05). All extracts presented moderate antiproliferative activity against the cell lines evaluated, HeLa being the most susceptible of them. However, ALGE and SULGE were the most active too. About antimicrobial activity, SULGE (MIC90<800 μg/mL; MIC50 < 400 μg/mL), and SLGE (MIC50 < 1000 μg/mL) showed a moderate inhibitory effect against S. aureus. These findings provide new information about the seasonal effect on the PC and biological properties of LG extracts. Clearly, antioxidant activity was the most important with respect to the other two. |
url |
http://dx.doi.org/10.1155/2018/2738489 |
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