The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method
Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women. Materials...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Ilam University of Medical Sciences
2018-03-01
|
Series: | Journal of Basic Research in Medical Sciences |
Subjects: | |
Online Access: | http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-5&slc_lang=en&sid=1 |
id |
doaj-d335cb3a395e4a0dbb864a70d77fcd75 |
---|---|
record_format |
Article |
spelling |
doaj-d335cb3a395e4a0dbb864a70d77fcd752020-11-24T21:46:39ZengIlam University of Medical SciencesJournal of Basic Research in Medical Sciences2383-05062383-09722018-03-01522732The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR methodAazam Khani Daramroodi0Fatemeh Keshavarzi1Farshid Raissi2 Department of Biology, Kurdistan Science and Research Branch, Islamic Azad University, Sanandaj, Iran Department of Genetics, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran Department of Pathology, Faculty of Medicine, Kermanshah University of Medical Science, Kermanshah, Iran Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women. Materials and methods: Hundred pregnant women, at the 35th and 37th weeks of pregnancy, were selected from the Obstetrics and Gynecology Unit of the Moatazedi and Shahid Chamran Hospitals in Kermanshah province. Specimens were collected from vaginal introitus and investigated by selective culture and colony PCR methods. Then, antibiotic resistance tests were performed according to the latest guidelines of Clinical and Laboratory Standards Institute (CLSI). Results: Prevalence of GBS colonization was shown to be 5% and 6% by the culture and colony PCR methods, respectively. Also, resistance rate to erythromycin, penicillin, vancomycin, and the clindamycin were determined to be 50%, 16.66%, 16.66% and 33.33 %, respectively. Moreover, the highest resistance was for erythromycin and the appropriate antibiotics were penicillin and then vancomycin. Conclusion: A higher prevalence of GBS colonization in pregnant women in the Kermanshah city of Iran was detected using colony PCR method compared to culture method.http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-5&slc_lang=en&sid=1Antibiotic ResistanceBCA geneColony-PCRGBS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aazam Khani Daramroodi Fatemeh Keshavarzi Farshid Raissi |
spellingShingle |
Aazam Khani Daramroodi Fatemeh Keshavarzi Farshid Raissi The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method Journal of Basic Research in Medical Sciences Antibiotic Resistance BCA gene Colony-PCR GBS |
author_facet |
Aazam Khani Daramroodi Fatemeh Keshavarzi Farshid Raissi |
author_sort |
Aazam Khani Daramroodi |
title |
The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method |
title_short |
The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method |
title_full |
The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method |
title_fullStr |
The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method |
title_full_unstemmed |
The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method |
title_sort |
investigation of antibiotic resistance and rapid detection of group b streptococcus (bca) from vaginal specimens of pregnant women by colony pcr method |
publisher |
Ilam University of Medical Sciences |
series |
Journal of Basic Research in Medical Sciences |
issn |
2383-0506 2383-0972 |
publishDate |
2018-03-01 |
description |
Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women.
Materials and methods: Hundred pregnant women, at the 35th and 37th weeks of pregnancy, were selected from the Obstetrics and Gynecology Unit of the Moatazedi and Shahid Chamran Hospitals in Kermanshah province. Specimens were collected from vaginal introitus and investigated by selective culture and colony PCR methods. Then, antibiotic resistance tests were performed according to the latest guidelines of Clinical and Laboratory Standards Institute (CLSI).
Results: Prevalence of GBS colonization was shown to be 5% and 6% by the culture and colony PCR methods, respectively. Also, resistance rate to erythromycin, penicillin, vancomycin, and the clindamycin were determined to be 50%, 16.66%, 16.66% and 33.33 %, respectively. Moreover, the highest resistance was for erythromycin and the appropriate antibiotics were penicillin and then vancomycin.
Conclusion: A higher prevalence of GBS colonization in pregnant women in the Kermanshah city of Iran was detected using colony PCR method compared to culture method. |
topic |
Antibiotic Resistance BCA gene Colony-PCR GBS |
url |
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-5&slc_lang=en&sid=1 |
work_keys_str_mv |
AT aazamkhanidaramroodi theinvestigationofantibioticresistanceandrapiddetectionofgroupbstreptococcusbcafromvaginalspecimensofpregnantwomenbycolonypcrmethod AT fatemehkeshavarzi theinvestigationofantibioticresistanceandrapiddetectionofgroupbstreptococcusbcafromvaginalspecimensofpregnantwomenbycolonypcrmethod AT farshidraissi theinvestigationofantibioticresistanceandrapiddetectionofgroupbstreptococcusbcafromvaginalspecimensofpregnantwomenbycolonypcrmethod AT aazamkhanidaramroodi investigationofantibioticresistanceandrapiddetectionofgroupbstreptococcusbcafromvaginalspecimensofpregnantwomenbycolonypcrmethod AT fatemehkeshavarzi investigationofantibioticresistanceandrapiddetectionofgroupbstreptococcusbcafromvaginalspecimensofpregnantwomenbycolonypcrmethod AT farshidraissi investigationofantibioticresistanceandrapiddetectionofgroupbstreptococcusbcafromvaginalspecimensofpregnantwomenbycolonypcrmethod |
_version_ |
1725900892483354624 |