Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy
A novel, simple, and highly sensitive method was developed to detect the concentration of tyrosine-derived azo dye indirectly using silver nanocubes (AgNCs) as a substrate on a super-hydrophobic silver film by surface-enhanced Raman spectroscopy (SERS). Diazotization-coupling reaction occurred betwe...
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doaj-d33682c2b42f4a35a9064aa0f5e29dc72020-11-25T00:26:35ZengMDPI AGNanomaterials2079-49912018-06-018640010.3390/nano8060400nano8060400Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman SpectroscopyYudong Lu0Dechan Lu1Ruiyun You2Jialing Liu3Luqiang Huang4Jingqian Su5Shangyuan Feng6Fujian Key Laboratory of Polymer Materials, College of Chemistry and Materials Science, Fuzhou 350007, Fujian, ChinaFujian Key Laboratory of Polymer Materials, College of Chemistry and Materials Science, Fuzhou 350007, Fujian, ChinaFujian Key Laboratory of Polymer Materials, College of Chemistry and Materials Science, Fuzhou 350007, Fujian, ChinaCenter of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, Fujian Normal University, Fuzhou 350117, Fujian, ChinaCenter of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, Fujian Normal University, Fuzhou 350117, Fujian, ChinaCenter of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, Fujian Normal University, Fuzhou 350117, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou 350117, Fujian, ChinaA novel, simple, and highly sensitive method was developed to detect the concentration of tyrosine-derived azo dye indirectly using silver nanocubes (AgNCs) as a substrate on a super-hydrophobic silver film by surface-enhanced Raman spectroscopy (SERS). Diazotization-coupling reaction occurred between diazonium ions and the phenolic tyrosine, resulting in three new typical peaks in the SERS spectrum of the azo dye that was formed on the AgNCs, indicating strong SERS activity. Subsequently, the limit of detection of this approach was as low as 10−12 M for tyrosine. Moreover, the SERS intensities of the three typical SERS signals of the analyte were linearly correlated with the logarithm of concentration of the Tyrosine. The proposed method shows great potential for tyrosine detection in the urine samples of normal humans.http://www.mdpi.com/2079-4991/8/6/400silver nanocubesdiazotization-coupling reactionTyrosineSurface enhanced Raman scattering |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yudong Lu Dechan Lu Ruiyun You Jialing Liu Luqiang Huang Jingqian Su Shangyuan Feng |
spellingShingle |
Yudong Lu Dechan Lu Ruiyun You Jialing Liu Luqiang Huang Jingqian Su Shangyuan Feng Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy Nanomaterials silver nanocubes diazotization-coupling reaction Tyrosine Surface enhanced Raman scattering |
author_facet |
Yudong Lu Dechan Lu Ruiyun You Jialing Liu Luqiang Huang Jingqian Su Shangyuan Feng |
author_sort |
Yudong Lu |
title |
Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy |
title_short |
Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy |
title_full |
Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy |
title_fullStr |
Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy |
title_full_unstemmed |
Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy |
title_sort |
diazotization-coupling reaction-based determination of tyrosine in urine using ag nanocubes by surface-enhanced raman spectroscopy |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2018-06-01 |
description |
A novel, simple, and highly sensitive method was developed to detect the concentration of tyrosine-derived azo dye indirectly using silver nanocubes (AgNCs) as a substrate on a super-hydrophobic silver film by surface-enhanced Raman spectroscopy (SERS). Diazotization-coupling reaction occurred between diazonium ions and the phenolic tyrosine, resulting in three new typical peaks in the SERS spectrum of the azo dye that was formed on the AgNCs, indicating strong SERS activity. Subsequently, the limit of detection of this approach was as low as 10−12 M for tyrosine. Moreover, the SERS intensities of the three typical SERS signals of the analyte were linearly correlated with the logarithm of concentration of the Tyrosine. The proposed method shows great potential for tyrosine detection in the urine samples of normal humans. |
topic |
silver nanocubes diazotization-coupling reaction Tyrosine Surface enhanced Raman scattering |
url |
http://www.mdpi.com/2079-4991/8/6/400 |
work_keys_str_mv |
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