Microwave-Assisted Extraction of Multiple Trace Levels of Intermediate Metabolites for Camptothecin Biosynthesis in <em>Camptotheca acuminata</em> and Their Simultaneous Determination by HPLC-LTQ-Orbitrap-MS/MS and HPLC-TSQ-MS

Microwave-Assisted Extraction of Multiple Trace Levels of Intermediate Metabolites for Camptothecin Biosynthesis in <em>Camptotheca acuminata</em> and Their Simultaneous Determination by HPLC-LTQ-Orbitrap-MS/MS and HPLC-TSQ-MS

Camptothecin (CPT) has strong antitumor activity and is used as an anticancer therapeutic agent. To better understand and decipher the pathway of CPT biosynthesis in <i>Camptotheca acuminata</i>, the main purpose here was focused on creating an effective extraction strategy for a rich in...

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Bibliographic Details
Main Authors:	Zhaoxia Jin, Ruyi Wan, Ruxue Yan, Yingying Su, Honglan Huang, Lihan Zi, Fang Yu
Format:	Article
Language:	English
Published:	MDPI AG 2019-02-01
Series:	Molecules
Subjects:	microwave assisted extraction Taguchi experimental design metabolites camptothecin HPLC-LTQ-MS/MS
Online Access:	https://www.mdpi.com/1420-3049/24/4/815

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