Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo)
Domestic turkey production generally utilises only a few genetically improved lines, and local breeds are severely endangered as a result. Furthermore, the genetic resources of domestic turkeys have not been properly investigated, which could, ultimately, lead to the extinction of local breeds and n...
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doaj-d35e77b4524840cc89c44e800b4696072021-06-25T10:30:43ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2020-12-0119139239810.1080/1828051X.2020.17456951745695Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo)Amado Manuel Canales Vergara0Vincenzo Landi1Juan Vicente Delgado Bermejo2Amparo Martínez Martínez3Patricia Cervantes Acosta4Agueda Pons Barros5Daniele Bigi6Phillip Sponenberg7Mostafa Helal8Mohammad Hossein Banabazi9Maria Esperanza Camacho Vallejo10Department of Genetics, Faculty of Veterinary Sciences, University of CórdobaAnimal Breeding Consulting S.L. Parque Científico Tecnológico de Córdoba c/Astrónoma Cecilia PayneDepartment of Genetics, Faculty of Veterinary Sciences, University of CórdobaDepartment of Genetics, Faculty of Veterinary Sciences, University of CórdobaFacultad de Medicina Veterinaria y Zootecnia, Universidad VeracruzanaServei de Millora Agrària (SEMILLA)Department of Agricultural and Food Sciences, Università di BolognaDepartment of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary MedicineDepartment of Animal Production, Cairo UniversityDepartment of Biotechnology, Animal Science Research Institute of IRAN (ASRI), Agricultural Research, Education & Extension Organization (AREEO)Instituto de Investigación y Formación Agraria y Pesquera (IFAPA)Domestic turkey production generally utilises only a few genetically improved lines, and local breeds are severely endangered as a result. Furthermore, the genetic resources of domestic turkeys have not been properly investigated, which could, ultimately, lead to the extinction of local breeds and negatively affect their corresponding genetic diversity and environmental adaptation. Although, several microsatellite markers have been designed for mapping and quantitative trait locus analysis, there is no standard panel of markers for genetic characterisation or genetic diversity assessment. Accordingly, the present study aimed to develop a set of polymorphic microsatellite markers that could be used for international turkey population studies. Thirty-nine microsatellites were selected based on polymorphism, DNA sequence and chromosome position, as well as on amplification efficiency, success rate and the absence of nonspecific amplification. The markers were screened using 105 DNA samples from local turkey breeds from Mexico, the United States, Italy, Brazil, Egypt and Spain. A total of 401 alleles were identified, with a mean number of alleles per marker of 10.28 ± 4.25. All microsatellites were polymorphic, with at least four alleles and no more than 19 alleles. Furthermore, allelic richness ranged from 3.810 to 17.985, mean heterozygosity ranged from 0.452 ± 0.229 to 0.667 ± 0.265, polymorphic information content values ranged from 0.213 (MNT264) to 0.850 (RHT0024) and the mean Fis value was 0.322. Overall, the panel was highly polymorphic and exhibited moderate Hardy–Weinberg disequilibrium, thereby indicating its value as a tool for biodiversity and population structure studies that could play an important role in promoting the conservation of local turkey breeds.Highlights Important genetic resources reside within indigenous turkey populations. These are linked to historic heritage production values and breeds. It is important to preserve this heritage and genetic diversity, which threatens to be lost as production systems focus on production characteristics. Microsatellite markers, even though, they are now replaced by single nucleotide polymorphism automatic genotyping platforms in many fields of genetics, remain a viable alternative thanks to their cheapness and simplicity of study which makes them particularly useful when the population to be studied lacks information of the prior genetic structure.http://dx.doi.org/10.1080/1828051X.2020.1745695meleagris gallopavo; microsatellitesbiodiversitypower of exclusion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amado Manuel Canales Vergara Vincenzo Landi Juan Vicente Delgado Bermejo Amparo Martínez Martínez Patricia Cervantes Acosta Agueda Pons Barros Daniele Bigi Phillip Sponenberg Mostafa Helal Mohammad Hossein Banabazi Maria Esperanza Camacho Vallejo |
spellingShingle |
Amado Manuel Canales Vergara Vincenzo Landi Juan Vicente Delgado Bermejo Amparo Martínez Martínez Patricia Cervantes Acosta Agueda Pons Barros Daniele Bigi Phillip Sponenberg Mostafa Helal Mohammad Hossein Banabazi Maria Esperanza Camacho Vallejo Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo) Italian Journal of Animal Science meleagris gallopavo; microsatellites biodiversity power of exclusion |
author_facet |
Amado Manuel Canales Vergara Vincenzo Landi Juan Vicente Delgado Bermejo Amparo Martínez Martínez Patricia Cervantes Acosta Agueda Pons Barros Daniele Bigi Phillip Sponenberg Mostafa Helal Mohammad Hossein Banabazi Maria Esperanza Camacho Vallejo |
author_sort |
Amado Manuel Canales Vergara |
title |
Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo) |
title_short |
Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo) |
title_full |
Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo) |
title_fullStr |
Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo) |
title_full_unstemmed |
Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo) |
title_sort |
design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (meleagris gallopavo gallopavo) |
publisher |
Taylor & Francis Group |
series |
Italian Journal of Animal Science |
issn |
1594-4077 1828-051X |
publishDate |
2020-12-01 |
description |
Domestic turkey production generally utilises only a few genetically improved lines, and local breeds are severely endangered as a result. Furthermore, the genetic resources of domestic turkeys have not been properly investigated, which could, ultimately, lead to the extinction of local breeds and negatively affect their corresponding genetic diversity and environmental adaptation. Although, several microsatellite markers have been designed for mapping and quantitative trait locus analysis, there is no standard panel of markers for genetic characterisation or genetic diversity assessment. Accordingly, the present study aimed to develop a set of polymorphic microsatellite markers that could be used for international turkey population studies. Thirty-nine microsatellites were selected based on polymorphism, DNA sequence and chromosome position, as well as on amplification efficiency, success rate and the absence of nonspecific amplification. The markers were screened using 105 DNA samples from local turkey breeds from Mexico, the United States, Italy, Brazil, Egypt and Spain. A total of 401 alleles were identified, with a mean number of alleles per marker of 10.28 ± 4.25. All microsatellites were polymorphic, with at least four alleles and no more than 19 alleles. Furthermore, allelic richness ranged from 3.810 to 17.985, mean heterozygosity ranged from 0.452 ± 0.229 to 0.667 ± 0.265, polymorphic information content values ranged from 0.213 (MNT264) to 0.850 (RHT0024) and the mean Fis value was 0.322. Overall, the panel was highly polymorphic and exhibited moderate Hardy–Weinberg disequilibrium, thereby indicating its value as a tool for biodiversity and population structure studies that could play an important role in promoting the conservation of local turkey breeds.Highlights Important genetic resources reside within indigenous turkey populations. These are linked to historic heritage production values and breeds. It is important to preserve this heritage and genetic diversity, which threatens to be lost as production systems focus on production characteristics. Microsatellite markers, even though, they are now replaced by single nucleotide polymorphism automatic genotyping platforms in many fields of genetics, remain a viable alternative thanks to their cheapness and simplicity of study which makes them particularly useful when the population to be studied lacks information of the prior genetic structure. |
topic |
meleagris gallopavo; microsatellites biodiversity power of exclusion |
url |
http://dx.doi.org/10.1080/1828051X.2020.1745695 |
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