Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or H<sub>2</sub>O<sub>2</sub>-Induced Acute Lung Injury
Jojoba (Simmondsia chinensis (Link) C.K. Schneid) is a dioecious plant in desert and semi-desert areas, e.g., the Ismailia Desert in Egypt. Jojoba oil (JJBO) is a natural slight yellow oil with the functions of skin barrier repairing and wound healing, which is dermally applied as a traditional medi...
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doaj-d360406f1c6141e3b71bf252144bc4552021-04-02T23:06:17ZengMDPI AGPharmaceutics1999-49232021-04-011348648610.3390/pharmaceutics13040486Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or H<sub>2</sub>O<sub>2</sub>-Induced Acute Lung InjuryGuoli Zhang0Fei Xie1Yunbo Sun2Xiang Yu3Zhimei Xiao4Rongzhen Fang5Jingfei Li6Qian Li7Lina Du8Yiguang Jin9Graduates Department, Anhui Medical University, Hefei 230032, ChinaDepartment of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing 100853, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, ChinaGraduates Department, Anhui Medical University, Hefei 230032, ChinaGraduates Department, Anhui Medical University, Hefei 230032, ChinaJojoba (Simmondsia chinensis (Link) C.K. Schneid) is a dioecious plant in desert and semi-desert areas, e.g., the Ismailia Desert in Egypt. Jojoba oil (JJBO) is a natural slight yellow oil with the functions of skin barrier repairing and wound healing, which is dermally applied as a traditional medication or cosmetic in the Middle East. The objective of this study was to prepare JJBO dry nanoemulsion powders (JNDs) and investigate their anti-acute lung injury effects. JJBO nanoemulsions (JNEs) were prepared and then lyophilized to JNDs and the properties and simulated lung deposition were measured. Rat acute lung injury (ALI) models were established after intratracheal (i.t.) administration of lipopolysaccharide (LPS) or hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). JNDs and dexamethasone (DXM) solutions were also i.t. administered to the rats. The pathological states of lung tissues were checked. Inflammatory and oxidative factors in the lung tissues were determined using ELISA methods. NF-κB p65 and caspase-3 were measured with a Western blotting method and an immunohistochemical method, respectively. JNDs had an appropriate mass median aerodynamic diameter (MMAD) of 4.17 µm and a fine particle fraction (FPF) of 39.11%. JNDs showed higher anti-inflammatory effect on LPS-induced ALI than DXM with a decrease in total protein content and down-regulation of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and NF-κB p65. JNDs also showed higher anti-inflammatory and anti-oxidation effect on H<sub>2</sub>O<sub>2</sub>-induced ALI than DXM with elimination of reactive oxygen species (ROS), increasing of superoxide dismutase (SOD), decrease in of lipid peroxide malondialdehyde (MDA) and glutathione (GSH), and inhibition of caspase-3 expression. Moreover, i.t. JNDs attenuated bleeding and infiltrations of the inflammatory cells in the two ALI models. JNDs are a promising natural oil-contained inhalable medication for the treatment of LPS- or H<sub>2</sub>O<sub>2</sub>-induced ALI.https://www.mdpi.com/1999-4923/13/4/486jojoba oilacute lung injurynanoemulsiondry powder inhalerhydrogen peroxidelipopolysaccharide (LPS) |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guoli Zhang Fei Xie Yunbo Sun Xiang Yu Zhimei Xiao Rongzhen Fang Jingfei Li Qian Li Lina Du Yiguang Jin |
spellingShingle |
Guoli Zhang Fei Xie Yunbo Sun Xiang Yu Zhimei Xiao Rongzhen Fang Jingfei Li Qian Li Lina Du Yiguang Jin Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or H<sub>2</sub>O<sub>2</sub>-Induced Acute Lung Injury Pharmaceutics jojoba oil acute lung injury nanoemulsion dry powder inhaler hydrogen peroxide lipopolysaccharide (LPS) |
author_facet |
Guoli Zhang Fei Xie Yunbo Sun Xiang Yu Zhimei Xiao Rongzhen Fang Jingfei Li Qian Li Lina Du Yiguang Jin |
author_sort |
Guoli Zhang |
title |
Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or H<sub>2</sub>O<sub>2</sub>-Induced Acute Lung Injury |
title_short |
Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or H<sub>2</sub>O<sub>2</sub>-Induced Acute Lung Injury |
title_full |
Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or H<sub>2</sub>O<sub>2</sub>-Induced Acute Lung Injury |
title_fullStr |
Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or H<sub>2</sub>O<sub>2</sub>-Induced Acute Lung Injury |
title_full_unstemmed |
Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or H<sub>2</sub>O<sub>2</sub>-Induced Acute Lung Injury |
title_sort |
inhalable jojoba oil dry nanoemulsion powders for the treatment of lipopolysaccharide- or h<sub>2</sub>o<sub>2</sub>-induced acute lung injury |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2021-04-01 |
description |
Jojoba (Simmondsia chinensis (Link) C.K. Schneid) is a dioecious plant in desert and semi-desert areas, e.g., the Ismailia Desert in Egypt. Jojoba oil (JJBO) is a natural slight yellow oil with the functions of skin barrier repairing and wound healing, which is dermally applied as a traditional medication or cosmetic in the Middle East. The objective of this study was to prepare JJBO dry nanoemulsion powders (JNDs) and investigate their anti-acute lung injury effects. JJBO nanoemulsions (JNEs) were prepared and then lyophilized to JNDs and the properties and simulated lung deposition were measured. Rat acute lung injury (ALI) models were established after intratracheal (i.t.) administration of lipopolysaccharide (LPS) or hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). JNDs and dexamethasone (DXM) solutions were also i.t. administered to the rats. The pathological states of lung tissues were checked. Inflammatory and oxidative factors in the lung tissues were determined using ELISA methods. NF-κB p65 and caspase-3 were measured with a Western blotting method and an immunohistochemical method, respectively. JNDs had an appropriate mass median aerodynamic diameter (MMAD) of 4.17 µm and a fine particle fraction (FPF) of 39.11%. JNDs showed higher anti-inflammatory effect on LPS-induced ALI than DXM with a decrease in total protein content and down-regulation of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and NF-κB p65. JNDs also showed higher anti-inflammatory and anti-oxidation effect on H<sub>2</sub>O<sub>2</sub>-induced ALI than DXM with elimination of reactive oxygen species (ROS), increasing of superoxide dismutase (SOD), decrease in of lipid peroxide malondialdehyde (MDA) and glutathione (GSH), and inhibition of caspase-3 expression. Moreover, i.t. JNDs attenuated bleeding and infiltrations of the inflammatory cells in the two ALI models. JNDs are a promising natural oil-contained inhalable medication for the treatment of LPS- or H<sub>2</sub>O<sub>2</sub>-induced ALI. |
topic |
jojoba oil acute lung injury nanoemulsion dry powder inhaler hydrogen peroxide lipopolysaccharide (LPS) |
url |
https://www.mdpi.com/1999-4923/13/4/486 |
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