PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana
Parasitic root-knot nematodes transform the host’s vascular cells into permanent feeding giant cells (GCs) to withdraw nutrients from the host plants. GCs are multinucleated metabolically active cells with distinctive cell wall structures; however, the genetic regulation of GC formation is largely u...
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doaj-d3868854f6d64765a41945c624113bb02021-10-06T07:26:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-10-011210.3389/fpls.2021.755610755610PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thalianaReira Suzuki0Mizuki Yamada1Takumi Higaki2Takumi Higaki3Mitsuhiro Aida4Mitsuhiro Aida5Minoru Kubo6Minoru Kubo7Allen Yi-Lun Tsai8Allen Yi-Lun Tsai9Shinichiro Sawa10Shinichiro Sawa11Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, JapanInternational Research Organization for Advanced Science and Technology, Kumamoto University, Kumamoto, JapanInternational Research Organization for Advanced Science and Technology, Kumamoto University, Kumamoto, JapanInternational Research Center for Agricultural and Environmental Biology, Kumamoto University, Kumamoto, JapanInternational Research Organization for Advanced Science and Technology, Kumamoto University, Kumamoto, JapanInternational Research Center for Agricultural and Environmental Biology, Kumamoto University, Kumamoto, JapanDepartment of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, JapanCenter for Digital Green-innovation, Nara Institute of Science and Technology, Nara, JapanDepartment of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, JapanInternational Research Center for Agricultural and Environmental Biology, Kumamoto University, Kumamoto, JapanDepartment of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, JapanInternational Research Center for Agricultural and Environmental Biology, Kumamoto University, Kumamoto, JapanParasitic root-knot nematodes transform the host’s vascular cells into permanent feeding giant cells (GCs) to withdraw nutrients from the host plants. GCs are multinucleated metabolically active cells with distinctive cell wall structures; however, the genetic regulation of GC formation is largely unknown. In this study, the functions of the Arabidopsis thaliana transcription factor PUCHI during GC development were investigated. PUCHI expression was shown to be induced in early developing galls, suggesting the importance of the PUCHI gene in gall formation. Despite the puchi mutant not differing significantly from the wild type in nematode invasion and reproduction rates, puchi GC cell walls appeared to be thicker and lobate when compared to the wild type, while the cell membrane sometimes formed invaginations. In three-dimensional (3D) reconstructions of puchi GCs, they appeared to be more irregularly shaped than those in the wild type, with noticeable cell-surface protrusions and folds. Interestingly, the loss-of-function mutant of 3-KETOACYL-COA SYNTHASE 1 showed GC morphology and cell wall defects similar to those of the puchi mutant, suggesting that PUCHI may regulate GC development via very long chain fatty acid synthesis.https://www.frontiersin.org/articles/10.3389/fpls.2021.755610/fullplant-parasitic nematodecell wallcell morphologyVLCFAplant-pathogen interaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Reira Suzuki Mizuki Yamada Takumi Higaki Takumi Higaki Mitsuhiro Aida Mitsuhiro Aida Minoru Kubo Minoru Kubo Allen Yi-Lun Tsai Allen Yi-Lun Tsai Shinichiro Sawa Shinichiro Sawa |
spellingShingle |
Reira Suzuki Mizuki Yamada Takumi Higaki Takumi Higaki Mitsuhiro Aida Mitsuhiro Aida Minoru Kubo Minoru Kubo Allen Yi-Lun Tsai Allen Yi-Lun Tsai Shinichiro Sawa Shinichiro Sawa PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana Frontiers in Plant Science plant-parasitic nematode cell wall cell morphology VLCFA plant-pathogen interaction |
author_facet |
Reira Suzuki Mizuki Yamada Takumi Higaki Takumi Higaki Mitsuhiro Aida Mitsuhiro Aida Minoru Kubo Minoru Kubo Allen Yi-Lun Tsai Allen Yi-Lun Tsai Shinichiro Sawa Shinichiro Sawa |
author_sort |
Reira Suzuki |
title |
PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana |
title_short |
PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana |
title_full |
PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana |
title_fullStr |
PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana |
title_full_unstemmed |
PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana |
title_sort |
puchi regulates giant cell morphology during root-knot nematode infection in arabidopsis thaliana |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-10-01 |
description |
Parasitic root-knot nematodes transform the host’s vascular cells into permanent feeding giant cells (GCs) to withdraw nutrients from the host plants. GCs are multinucleated metabolically active cells with distinctive cell wall structures; however, the genetic regulation of GC formation is largely unknown. In this study, the functions of the Arabidopsis thaliana transcription factor PUCHI during GC development were investigated. PUCHI expression was shown to be induced in early developing galls, suggesting the importance of the PUCHI gene in gall formation. Despite the puchi mutant not differing significantly from the wild type in nematode invasion and reproduction rates, puchi GC cell walls appeared to be thicker and lobate when compared to the wild type, while the cell membrane sometimes formed invaginations. In three-dimensional (3D) reconstructions of puchi GCs, they appeared to be more irregularly shaped than those in the wild type, with noticeable cell-surface protrusions and folds. Interestingly, the loss-of-function mutant of 3-KETOACYL-COA SYNTHASE 1 showed GC morphology and cell wall defects similar to those of the puchi mutant, suggesting that PUCHI may regulate GC development via very long chain fatty acid synthesis. |
topic |
plant-parasitic nematode cell wall cell morphology VLCFA plant-pathogen interaction |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.755610/full |
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