Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas Immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectives
<abstract language="eng">Immunoassay techniques provide simple, powerful and inexpensive methods for analysis of environmental contaminants. However, the acceptance of immunoassays is dependent on the clear demonstration of quality and validity compared to more traditional techniques...
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Sociedade Brasileira de Química
2005-06-01
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doaj-d399cf5263f24df694c610db85b7eddf2020-11-24T21:51:12ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642005-06-0128346247110.1590/S0100-40422005000300018Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas Immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectivesGilvanda Silva Nunes<abstract language="eng">Immunoassay techniques provide simple, powerful and inexpensive methods for analysis of environmental contaminants. However, the acceptance of immunoassays is dependent on the clear demonstration of quality and validity compared to more traditional techniques. In this review, primarily, the understanding and the fundamentals of immunoassay methods are given in order to make good use of immunoassays, especially of EIA tests. Special attention is given to the concepts related to the enzyme-linked immunosorbent assay (ELISA) formats, such as inhibition concentration at 50% (IC50), detection limit (LOD), cross-reactivity (CR %). It is also explained why some immunoassays are quantitative methods whereas others can only be used as screening methods. A list of main commercial kits for detection of priority pollutants is given in order to help analysts. Others formats, such as flow-injection immunoassay analysis (FIIA), immunoassay chromatography and immunosensors are also cited.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000300018immunoassaysenvironmental contaminantsmonitoring |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gilvanda Silva Nunes |
spellingShingle |
Gilvanda Silva Nunes Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas Immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectives Química Nova immunoassays environmental contaminants monitoring |
author_facet |
Gilvanda Silva Nunes |
author_sort |
Gilvanda Silva Nunes |
title |
Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas Immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectives |
title_short |
Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas Immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectives |
title_full |
Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas Immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectives |
title_fullStr |
Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas Immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectives |
title_full_unstemmed |
Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas Immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectives |
title_sort |
métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas immunochemical methods for analysis of environmental contaminants: concepts, state of the art and perpectives |
publisher |
Sociedade Brasileira de Química |
series |
Química Nova |
issn |
0100-4042 1678-7064 |
publishDate |
2005-06-01 |
description |
<abstract language="eng">Immunoassay techniques provide simple, powerful and inexpensive methods for analysis of environmental contaminants. However, the acceptance of immunoassays is dependent on the clear demonstration of quality and validity compared to more traditional techniques. In this review, primarily, the understanding and the fundamentals of immunoassay methods are given in order to make good use of immunoassays, especially of EIA tests. Special attention is given to the concepts related to the enzyme-linked immunosorbent assay (ELISA) formats, such as inhibition concentration at 50% (IC50), detection limit (LOD), cross-reactivity (CR %). It is also explained why some immunoassays are quantitative methods whereas others can only be used as screening methods. A list of main commercial kits for detection of priority pollutants is given in order to help analysts. Others formats, such as flow-injection immunoassay analysis (FIIA), immunoassay chromatography and immunosensors are also cited. |
topic |
immunoassays environmental contaminants monitoring |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000300018 |
work_keys_str_mv |
AT gilvandasilvanunes metodosimunoquimicosparaanalisedecontaminantesambientaisconceitosestadodaarteeperspectivasimmunochemicalmethodsforanalysisofenvironmentalcontaminantsconceptsstateoftheartandperpectives |
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