Protective effect of hydroalcohlic olive leaf extract on inhibition of Gentamicin-induced nephrotoxicity in rat
Background : Administration of Gentamicin is restricted due to its nephrotoxicity. Many studies have demonstrated that free radicals induced Gentamicin- nephrotoxicity. Olive leaf contains some antioxidant biophenols such as Oleuropin. In this study for the first time the effect of hydroalcohlic oli...
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Lorestan University of Medical Science
2011-12-01
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doaj-d3aa9393f6464385ad976f2dc76ea96d2020-11-24T21:48:21ZfasLorestan University of Medical ScienceYafteh1563-07732011-12-011336474Protective effect of hydroalcohlic olive leaf extract on inhibition of Gentamicin-induced nephrotoxicity in ratPoran Toolabi0Majid Tavafi1Soheila Ebrahimi2Sima Nasri3Hasan Ahmadvand4 Faculty of Basic Sciences and Agriculture, Payame Noor University of Tehran, Tehran, Iran, Pooran.toolabi@yahoo.com Faculty of Medicine, Lorestan University of Medical Sciences,Khorramabad,Iran Faculty of Basie Sciences, Payame Noor University of Babol,Babol, Iran Faculty of Basic Sciences and Agriculture, Payame Noor University of Tehran, Tehran, Iran, Pooran.toolabi@yahoo.com Faculty of medicine,Lorestan University of Medical Sciences,Khorramabad,Iran Background : Administration of Gentamicin is restricted due to its nephrotoxicity. Many studies have demonstrated that free radicals induced Gentamicin- nephrotoxicity. Olive leaf contains some antioxidant biophenols such as Oleuropin. In this study for the first time the effect of hydroalcohlic olive leaf extract on Gentamicin-induced nephrotoxicity was investigated. Materials and Methods: 35 male Sprague-Dowley rates were randomly divided into five groups. The first group served as a control and injected with the normal saline. Nephrotoxicity induced by Gentamicin (100 mg/kg) in experimental groups for 12 days. Three groups were treated with hydroalcohlic olive leaf extract (25, 50 and 100 mg/kg respectively). The rats were killed on the twelfth day.Plasma Malondialdehyde (MDA) and creatinine concentrations were measured. Kidney sections were prepared and stained with PAS method for histopathological studies. Results: hydroalcohlic olive leaf extract at 25, 50 and 100 mg/kg/d significantly reduced gentamicin-induced tubular necrosis (p<0.05) and inhibited decreases of proximal tubule volume density (p<0.05). hydroalcohlic olive leaf extract at 50 and 100 mg/kg/d significantly reduced lymphocyte infiltration in comparison with Gentamicin administrated rats (p<0.05). Gentamicin increased level of MDA. Treatment with extract (100 mg/kg/d), significantly reduced level of MDA compared with other groups (p<0.05).Use of extract at 25, 50 and 100 mg/kg/d significantly inhibited increase of serum creatinine (p<0.001). Treatment with extract (50 mg/kg/d), significantly inhibited increase of serum urea (p<0.05). Conclusion: Hydroaleohlic olive leaf extract can reduce Gentamicin-induced nephrotoxicity and this effect is probably depends on antioxidant properties of this extract.http://yafte.lums.ac.ir/browse.php?a_code=A-10-1-21&slc_lang=en&sid=1Olive leaf Gentamicin Nephrotoxicity Antioxidant |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Poran Toolabi Majid Tavafi Soheila Ebrahimi Sima Nasri Hasan Ahmadvand |
spellingShingle |
Poran Toolabi Majid Tavafi Soheila Ebrahimi Sima Nasri Hasan Ahmadvand Protective effect of hydroalcohlic olive leaf extract on inhibition of Gentamicin-induced nephrotoxicity in rat Yafteh Olive leaf Gentamicin Nephrotoxicity Antioxidant |
author_facet |
Poran Toolabi Majid Tavafi Soheila Ebrahimi Sima Nasri Hasan Ahmadvand |
author_sort |
Poran Toolabi |
title |
Protective effect of hydroalcohlic olive leaf extract on inhibition of Gentamicin-induced nephrotoxicity in rat |
title_short |
Protective effect of hydroalcohlic olive leaf extract on inhibition of Gentamicin-induced nephrotoxicity in rat |
title_full |
Protective effect of hydroalcohlic olive leaf extract on inhibition of Gentamicin-induced nephrotoxicity in rat |
title_fullStr |
Protective effect of hydroalcohlic olive leaf extract on inhibition of Gentamicin-induced nephrotoxicity in rat |
title_full_unstemmed |
Protective effect of hydroalcohlic olive leaf extract on inhibition of Gentamicin-induced nephrotoxicity in rat |
title_sort |
protective effect of hydroalcohlic olive leaf extract on inhibition of gentamicin-induced nephrotoxicity in rat |
publisher |
Lorestan University of Medical Science |
series |
Yafteh |
issn |
1563-0773 |
publishDate |
2011-12-01 |
description |
Background : Administration of Gentamicin is restricted due to its nephrotoxicity. Many studies have demonstrated that free radicals induced Gentamicin- nephrotoxicity. Olive leaf contains some antioxidant biophenols such as Oleuropin. In this study for the first time the effect of hydroalcohlic olive leaf extract on Gentamicin-induced nephrotoxicity was investigated.
Materials and Methods: 35 male Sprague-Dowley rates were randomly divided into five groups. The first group served as a control and injected with the normal saline. Nephrotoxicity induced by Gentamicin (100 mg/kg) in experimental groups for 12 days. Three groups were treated with hydroalcohlic olive leaf extract (25, 50 and 100 mg/kg respectively). The rats were killed on the twelfth day.Plasma Malondialdehyde (MDA) and creatinine concentrations were measured. Kidney sections were prepared and stained with PAS method for histopathological studies.
Results: hydroalcohlic olive leaf extract at 25, 50 and 100 mg/kg/d significantly reduced gentamicin-induced tubular necrosis (p<0.05) and inhibited decreases of proximal tubule volume density (p<0.05). hydroalcohlic olive leaf extract at 50 and 100 mg/kg/d significantly reduced lymphocyte infiltration in comparison with Gentamicin administrated rats (p<0.05). Gentamicin increased level of MDA. Treatment with extract (100 mg/kg/d), significantly reduced level of MDA compared with other groups (p<0.05).Use of extract at 25, 50 and 100 mg/kg/d significantly inhibited increase of serum creatinine (p<0.001). Treatment with extract (50 mg/kg/d), significantly inhibited increase of serum urea (p<0.05).
Conclusion: Hydroaleohlic olive leaf extract can reduce Gentamicin-induced nephrotoxicity and this effect is probably depends on antioxidant properties of this extract. |
topic |
Olive leaf Gentamicin Nephrotoxicity Antioxidant |
url |
http://yafte.lums.ac.ir/browse.php?a_code=A-10-1-21&slc_lang=en&sid=1 |
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