Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal

Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal

Lipase was immobilized onto bacterial magnetosomes using glutaraldehyde cross-linking and confirmed by Fourier transform infrared spectrometry (FT-IR) and Scanning electron microscopy (SEM). Enzyme activity of immobilised lipase as well as free lipase was estimated by the release of p-nitro phenol d...

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Main Authors: Jobin John Jacob, K Suthindhiran
Format: Article
Language:English
Published: Elsevier 2020-03-01
Series:Biotechnology Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X1930414X
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spelling doaj-d3c5b952cdf04878b0c8891f6da967c02020-11-25T00:45:06ZengElsevierBiotechnology Reports2215-017X2020-03-0125Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removalJobin John Jacob0K Suthindhiran1Senior Research Fellow-DST, Marine Biotechnology and Bioproducts Laboratory, School of Bio Sciences and Technology, VIT, Vellore, 632014, Tamil Nadu, IndiaMarine Biotechnology and Bioproducts Laboratory, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India; Corresponding author.Lipase was immobilized onto bacterial magnetosomes using glutaraldehyde cross-linking and confirmed by Fourier transform infrared spectrometry (FT-IR) and Scanning electron microscopy (SEM). Enzyme activity of immobilised lipase as well as free lipase was estimated by the release of p-nitro phenol due to the hydrolysis of p-nitro phenyl acetate (pNPA). The immobilisation yield of lipase onto magnetosome was found to be 88 %. The optimal pH (7) and temperature (40 °C) for activity was standardised and found to be similar to free lipase. The stored immobilized lipase maintained higher activity even after 30 days at a temperature of 4 °C whereas compared to free lipase. Immobilized lipase found to have removed vegetable oil stain and showed higher cleaning efficiency when compared to free lipase. The results suggest that bacterial magnetosome displays great potential as a support material for the immobilization of industrial enzymes such as lipase. Keywords: Enzyme activity, Lipase, Glutaraldehyde, Immobilization, Magnetosome, Stain removalhttp://www.sciencedirect.com/science/article/pii/S2215017X1930414X
collection DOAJ
language English
format Article
sources DOAJ
author Jobin John Jacob
K Suthindhiran
spellingShingle Jobin John Jacob
K Suthindhiran
Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal
Biotechnology Reports
author_facet Jobin John Jacob
K Suthindhiran
author_sort Jobin John Jacob
title Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal
title_short Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal
title_full Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal
title_fullStr Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal
title_full_unstemmed Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal
title_sort immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal
publisher Elsevier
series Biotechnology Reports
issn 2215-017X
publishDate 2020-03-01
description Lipase was immobilized onto bacterial magnetosomes using glutaraldehyde cross-linking and confirmed by Fourier transform infrared spectrometry (FT-IR) and Scanning electron microscopy (SEM). Enzyme activity of immobilised lipase as well as free lipase was estimated by the release of p-nitro phenol due to the hydrolysis of p-nitro phenyl acetate (pNPA). The immobilisation yield of lipase onto magnetosome was found to be 88 %. The optimal pH (7) and temperature (40 °C) for activity was standardised and found to be similar to free lipase. The stored immobilized lipase maintained higher activity even after 30 days at a temperature of 4 °C whereas compared to free lipase. Immobilized lipase found to have removed vegetable oil stain and showed higher cleaning efficiency when compared to free lipase. The results suggest that bacterial magnetosome displays great potential as a support material for the immobilization of industrial enzymes such as lipase. Keywords: Enzyme activity, Lipase, Glutaraldehyde, Immobilization, Magnetosome, Stain removal
url http://www.sciencedirect.com/science/article/pii/S2215017X1930414X
work_keys_str_mv AT jobinjohnjacob immobilisationoflipaseenzymeontobacterialmagnetosomesforstainremoval
AT ksuthindhiran immobilisationoflipaseenzymeontobacterialmagnetosomesforstainremoval
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