Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis
The mechanisms underlying the immunopathology of tuberculous meningitis (TBM), the most severe clinical form of extrapulmonary tuberculosis (TB), are not understood. It is currently believed that the spread of Mycobacterium tuberculosis (Mtb) from the lung is an early event that occurs before the es...
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doaj-d3ea20e016da4096875013e7b87ae3cc2021-05-10T00:26:44ZengHindawi LimitedJournal of Immunology Research2314-71562021-01-01202110.1155/2021/5517856Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal TuberculosisJosé Alberto Choreño-Parra0Luis Armando Jiménez-Álvarez1Ellis Daniela Maldonado-Díaz2Graciela Cárdenas3Luis Alejandro Fernández-Lopez4José Luis Soto-Hernandez5Marcela Muñoz-Torrico6Gustavo Ramírez-Martínez7Alfredo Cruz-Lagunas8Armando Vega-López9María Lilia Domínguez-López10Carlos Sánchez-Garibay11Parménides Guadarrama-Ortíz12Silvia Giono13Luis Antonio Jiménez-Zamudio14Shabaana A. Khader15Ethel A. García-Latorre16Citlaltepetl Salinas-Lara17Joaquín Zúñiga18Instituto Politécnico NacionalInstituto Politécnico NacionalNeuroinfectology DepartmentNeuroinfectology DepartmentInstituto Politécnico NacionalNeuroinfectology DepartmentTuberculosis ClinicLaboratory of Immunobiology and GeneticsLaboratory of Immunobiology and GeneticsInstituto Politécnico NacionalInstituto Politécnico NacionalDepartment of NeuropathologyCentro Especializado en Neurocirugía y Neurociencias México (CENNM)Instituto Politécnico NacionalInstituto Politécnico NacionalDepartment of Molecular MicrobiologyInstituto Politécnico NacionalDepartment of NeuropathologyEscuela de Medicina y Ciencias de la SaludThe mechanisms underlying the immunopathology of tuberculous meningitis (TBM), the most severe clinical form of extrapulmonary tuberculosis (TB), are not understood. It is currently believed that the spread of Mycobacterium tuberculosis (Mtb) from the lung is an early event that occurs before the establishment of adaptive immunity. Hence, several innate immune mechanisms may participate in the containment of Mtb infection and prevent extrapulmonary disease manifestations. Natural killer (NK) cells participate in defensive processes that distinguish latent TB infection (LTBI) from active pulmonary TB (PTB). However, their role in TBM is unknown. Here, we performed a cross-sectional analysis of circulating NK cellCID="C008" value="s" phenotype in a prospective cohort of TBM patients (n=10) using flow cytometry. Also, we addressed the responses of memory-like NK cell subpopulations to the contact with Mtb antigens in vitro. Finally, we determined plasma levels of soluble NKG2D receptor ligands in our cohort of TBM patients by enzyme-linked immunosorbent assay (ELISA). Our comparative groups consisted of individuals with LTBI (n=11) and PTB (n=27) patients. We found that NK cells from TBM patients showed lower absolute frequencies, higher CD69 expression, and poor expansion of the CD45RO+ memory-like subpopulation upon Mtb exposure in vitro compared to LTBI individuals. In addition, a reduction in the frequency of CD56brightCD16- NK cells characterized TBM patients but not LTBI or PTB subjects. Our study expands on earlier reports about the role of NK cells in TBM showing a reduced frequency of cytokine-producing cells compared to LTBI and PTB.http://dx.doi.org/10.1155/2021/5517856 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
José Alberto Choreño-Parra Luis Armando Jiménez-Álvarez Ellis Daniela Maldonado-Díaz Graciela Cárdenas Luis Alejandro Fernández-Lopez José Luis Soto-Hernandez Marcela Muñoz-Torrico Gustavo Ramírez-Martínez Alfredo Cruz-Lagunas Armando Vega-López María Lilia Domínguez-López Carlos Sánchez-Garibay Parménides Guadarrama-Ortíz Silvia Giono Luis Antonio Jiménez-Zamudio Shabaana A. Khader Ethel A. García-Latorre Citlaltepetl Salinas-Lara Joaquín Zúñiga |
spellingShingle |
José Alberto Choreño-Parra Luis Armando Jiménez-Álvarez Ellis Daniela Maldonado-Díaz Graciela Cárdenas Luis Alejandro Fernández-Lopez José Luis Soto-Hernandez Marcela Muñoz-Torrico Gustavo Ramírez-Martínez Alfredo Cruz-Lagunas Armando Vega-López María Lilia Domínguez-López Carlos Sánchez-Garibay Parménides Guadarrama-Ortíz Silvia Giono Luis Antonio Jiménez-Zamudio Shabaana A. Khader Ethel A. García-Latorre Citlaltepetl Salinas-Lara Joaquín Zúñiga Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis Journal of Immunology Research |
author_facet |
José Alberto Choreño-Parra Luis Armando Jiménez-Álvarez Ellis Daniela Maldonado-Díaz Graciela Cárdenas Luis Alejandro Fernández-Lopez José Luis Soto-Hernandez Marcela Muñoz-Torrico Gustavo Ramírez-Martínez Alfredo Cruz-Lagunas Armando Vega-López María Lilia Domínguez-López Carlos Sánchez-Garibay Parménides Guadarrama-Ortíz Silvia Giono Luis Antonio Jiménez-Zamudio Shabaana A. Khader Ethel A. García-Latorre Citlaltepetl Salinas-Lara Joaquín Zúñiga |
author_sort |
José Alberto Choreño-Parra |
title |
Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis |
title_short |
Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis |
title_full |
Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis |
title_fullStr |
Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis |
title_full_unstemmed |
Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis |
title_sort |
phenotype of peripheral nk cells in latent, active, and meningeal tuberculosis |
publisher |
Hindawi Limited |
series |
Journal of Immunology Research |
issn |
2314-7156 |
publishDate |
2021-01-01 |
description |
The mechanisms underlying the immunopathology of tuberculous meningitis (TBM), the most severe clinical form of extrapulmonary tuberculosis (TB), are not understood. It is currently believed that the spread of Mycobacterium tuberculosis (Mtb) from the lung is an early event that occurs before the establishment of adaptive immunity. Hence, several innate immune mechanisms may participate in the containment of Mtb infection and prevent extrapulmonary disease manifestations. Natural killer (NK) cells participate in defensive processes that distinguish latent TB infection (LTBI) from active pulmonary TB (PTB). However, their role in TBM is unknown. Here, we performed a cross-sectional analysis of circulating NK cellCID="C008" value="s" phenotype in a prospective cohort of TBM patients (n=10) using flow cytometry. Also, we addressed the responses of memory-like NK cell subpopulations to the contact with Mtb antigens in vitro. Finally, we determined plasma levels of soluble NKG2D receptor ligands in our cohort of TBM patients by enzyme-linked immunosorbent assay (ELISA). Our comparative groups consisted of individuals with LTBI (n=11) and PTB (n=27) patients. We found that NK cells from TBM patients showed lower absolute frequencies, higher CD69 expression, and poor expansion of the CD45RO+ memory-like subpopulation upon Mtb exposure in vitro compared to LTBI individuals. In addition, a reduction in the frequency of CD56brightCD16- NK cells characterized TBM patients but not LTBI or PTB subjects. Our study expands on earlier reports about the role of NK cells in TBM showing a reduced frequency of cytokine-producing cells compared to LTBI and PTB. |
url |
http://dx.doi.org/10.1155/2021/5517856 |
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