Expression and Clinical Significance of REGγ in Gastric Cancer Tissue and Variously Differentiated Gastric Cancer Cell Lines

• Main Authors: Jia LI, Tian TIAN, Xiaoyi WANG
• Format: Article
• Language: English
• Published: China Anti-Cancer Association 2009-06-01
• Series: Cancer Biology & Medicine
• Subjects: gastric tumor; REGγ; immunohistochemistry; gene; protein.
• Online Access: http://www.cancerbiomed.org/index.php/cocr/article/view/207

OBJECTIVE To evaluate the REGγ expression in gastric cancer tissue and gastric cancer cell lines of various differentiation levels and its clinical signi fi cance. METHODS Immunohistochemistry was used to detect the expression of REGγ protein in 70 specimens of gastric cancer and 30 specimens of normal gastric mucosa. The relationship between the expression of REG γ protein and the biological behaviors of gastric cancer was analyzed. RT-PCR and Western blot were used to detect the mRNA level and the protein expression of REG γ in normal gastric cell line GES-1, well differentiated gastric cancer cell line MKN-28, moderately differentiated gastric cancer cell line SGC-7901 and poorly differentiated gastric cancer cell line BGC-823. RESULTS The expression rate of REGγ protein in gastric cancer tissue (52/70, 74.29%) was significantly higher than that in normal gastric tissue (12/30, 40%) (P < 0.01). The expression rate of REG γ was correlated with tumor size (P < 0.01), lymph node metastasis ( P < 0.05), diff erentiation degree ( P < 0.01), infi ltration depth ( P < 0.01) and distant metastasis (P < 0.05). RT-PCR analysis showed that the expression of REGγ mRNA was 0.459 ± 0.079 in the normal gastric mucosa cell line, 0.588 ± 0.118 in the well differentiated gastric cancer cell line, 0.715 ± 0.066 in the moderately differentiated gastric cancer cell line, and 0.873 ± 0.099 in the poorly differentiated gastric cancer cell line, showing a negative correla-tion between REGγ mRNA expression and di ff erentiation level (P < 0.05). Western blot analysis showed that the expression of REG γ protein was 0.712 ± 0.065 in the normal gastric mucosa cell line, 1.176 ± 0.185 in the well di ff erentiated gastric cancer cell line, 1.533 ± 0.127 in the moderately diff erentiated gastric cancer cell line, and 2.061 ± 0.398 in the poorly differentiated gastric cancer cell line, showing a negative correlation between REGγ protein expression and differentiation level (P < 0.05) . CONCLUSION REGγ is expressed in gastric cancer tissue and normal gastric tissue. In gastric cancer tissues, REG γ expression is positively correlated with the tumor size, lymph node metastasis, differentiation degree, infiltration depth and distant metastasis. Detecting the expression of REG γ mRNA and protein is helpful for early diagnosis and predicting prognosis of gastric cancer.