Expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: An observational study
Background: Matrix metalloproteinases (MMPs) are a group of host-derived zinc-dependent enzymes which mediates the destruction of the extracellular matrix. In periodontitis, there is excess production of MMPs associated with periodontal tissue destruction. The aim of this study was to estimate the l...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2021-01-01
|
Series: | Journal of Indian Society of Periodontology |
Subjects: | |
Online Access: | http://www.jisponline.com/article.asp?issn=0972-124X;year=2021;volume=25;issue=5;spage=386;epage=392;aulast=Ghosh |
id |
doaj-d40d6bdacce34346a3f13e0e319973b4 |
---|---|
record_format |
Article |
spelling |
doaj-d40d6bdacce34346a3f13e0e319973b42021-09-07T14:48:33ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2021-01-0125538639210.4103/jisp.jisp_811_20Expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: An observational studyPapita GhoshThamil Selvan MuthurajPrasanta BandyopadhyaySnehasikta SwarnakarPuja SarkarAbinaya VaratharajanBackground: Matrix metalloproteinases (MMPs) are a group of host-derived zinc-dependent enzymes which mediates the destruction of the extracellular matrix. In periodontitis, there is excess production of MMPs associated with periodontal tissue destruction. The aim of this study was to estimate the level MMP-9 in both active and latent form in gingival tissue (GT) samples collected from periodontitis patients with different rates of progression and compare it with healthy individuals. Materials and Methods: Sixty patients were selected and divided into three groups, 20 each: Group A (slowly/moderately progressing periodontitis), Group B (rapidly progressing periodontitis), and Group C (clinical periodontal health). Plaque index, gingival index, periodontal probing depth (PPD), and clinical attachment level were recorded. GT samples were collected from all 60 patients and MMP-9 expressions were measured using gelatin zymography and western blotting. Results: Levels of active MMP-9 (aMMP-9) and latent MMP-9 (lMMP-9) were significantly high in both Group A (GA) (aMMP-9: 2.05 arbitrary unit [AU]/lMMP-9: 2.54 AU) and Group B (GB) (aMMP-9: 1.32 AU/lMMP-9: 1.74 AU) when compared to that of Group C (GC) (aMMP-9: 0.93/lMMP-9: 1.08 AU). In GA, levels of aMMP-9 showed a significant correlation with PPD values. No other correlations were found. Conclusion: The levels of aMMP-9 and lMMP-9 were increased in both the types of periodontitis when compared with periodontally healthy individuals. A significant correlation was found between PPD and activities of aMMP-9 in slowly/moderately progressing periodontitis patients. However, further studies are required to confirm these findings.http://www.jisponline.com/article.asp?issn=0972-124X;year=2021;volume=25;issue=5;spage=386;epage=392;aulast=Ghosh92-kda gelatinase92-kda type iv collagenasegelatinase bmatrix metalloproteinase-9mmp-9periodontitis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Papita Ghosh Thamil Selvan Muthuraj Prasanta Bandyopadhyay Snehasikta Swarnakar Puja Sarkar Abinaya Varatharajan |
spellingShingle |
Papita Ghosh Thamil Selvan Muthuraj Prasanta Bandyopadhyay Snehasikta Swarnakar Puja Sarkar Abinaya Varatharajan Expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: An observational study Journal of Indian Society of Periodontology 92-kda gelatinase 92-kda type iv collagenase gelatinase b matrix metalloproteinase-9 mmp-9 periodontitis |
author_facet |
Papita Ghosh Thamil Selvan Muthuraj Prasanta Bandyopadhyay Snehasikta Swarnakar Puja Sarkar Abinaya Varatharajan |
author_sort |
Papita Ghosh |
title |
Expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: An observational study |
title_short |
Expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: An observational study |
title_full |
Expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: An observational study |
title_fullStr |
Expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: An observational study |
title_full_unstemmed |
Expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: An observational study |
title_sort |
expression of matrix metalloproteinase-9 in gingival tissue biopsy in patients with slowly/moderately and rapidly progressing periodontitis: an observational study |
publisher |
Wolters Kluwer Medknow Publications |
series |
Journal of Indian Society of Periodontology |
issn |
0972-124X |
publishDate |
2021-01-01 |
description |
Background: Matrix metalloproteinases (MMPs) are a group of host-derived zinc-dependent enzymes which mediates the destruction of the extracellular matrix. In periodontitis, there is excess production of MMPs associated with periodontal tissue destruction. The aim of this study was to estimate the level MMP-9 in both active and latent form in gingival tissue (GT) samples collected from periodontitis patients with different rates of progression and compare it with healthy individuals. Materials and Methods: Sixty patients were selected and divided into three groups, 20 each: Group A (slowly/moderately progressing periodontitis), Group B (rapidly progressing periodontitis), and Group C (clinical periodontal health). Plaque index, gingival index, periodontal probing depth (PPD), and clinical attachment level were recorded. GT samples were collected from all 60 patients and MMP-9 expressions were measured using gelatin zymography and western blotting. Results: Levels of active MMP-9 (aMMP-9) and latent MMP-9 (lMMP-9) were significantly high in both Group A (GA) (aMMP-9: 2.05 arbitrary unit [AU]/lMMP-9: 2.54 AU) and Group B (GB) (aMMP-9: 1.32 AU/lMMP-9: 1.74 AU) when compared to that of Group C (GC) (aMMP-9: 0.93/lMMP-9: 1.08 AU). In GA, levels of aMMP-9 showed a significant correlation with PPD values. No other correlations were found. Conclusion: The levels of aMMP-9 and lMMP-9 were increased in both the types of periodontitis when compared with periodontally healthy individuals. A significant correlation was found between PPD and activities of aMMP-9 in slowly/moderately progressing periodontitis patients. However, further studies are required to confirm these findings. |
topic |
92-kda gelatinase 92-kda type iv collagenase gelatinase b matrix metalloproteinase-9 mmp-9 periodontitis |
url |
http://www.jisponline.com/article.asp?issn=0972-124X;year=2021;volume=25;issue=5;spage=386;epage=392;aulast=Ghosh |
work_keys_str_mv |
AT papitaghosh expressionofmatrixmetalloproteinase9ingingivaltissuebiopsyinpatientswithslowlymoderatelyandrapidlyprogressingperiodontitisanobservationalstudy AT thamilselvanmuthuraj expressionofmatrixmetalloproteinase9ingingivaltissuebiopsyinpatientswithslowlymoderatelyandrapidlyprogressingperiodontitisanobservationalstudy AT prasantabandyopadhyay expressionofmatrixmetalloproteinase9ingingivaltissuebiopsyinpatientswithslowlymoderatelyandrapidlyprogressingperiodontitisanobservationalstudy AT snehasiktaswarnakar expressionofmatrixmetalloproteinase9ingingivaltissuebiopsyinpatientswithslowlymoderatelyandrapidlyprogressingperiodontitisanobservationalstudy AT pujasarkar expressionofmatrixmetalloproteinase9ingingivaltissuebiopsyinpatientswithslowlymoderatelyandrapidlyprogressingperiodontitisanobservationalstudy AT abinayavaratharajan expressionofmatrixmetalloproteinase9ingingivaltissuebiopsyinpatientswithslowlymoderatelyandrapidlyprogressingperiodontitisanobservationalstudy |
_version_ |
1717763286591602688 |